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AB121943

Anti-WEE2 antibody

3

(1 Review)

|

(3 Publications)

Rabbit Polyclonal WEE2 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WEE2 aa 300-400.

View Alternative Names

WEE1B, WEE2, Wee1-like protein kinase 2, Wee1-like protein kinase 1B, Wee1B kinase

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labeling WEE2 with ab121943 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling WEE2 with ab121943 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling WEE2 with ab121943 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry - Anti-WEE2 antibody (AB121943)
  • ICC

Supplier Data

Immunocytochemistry - Anti-WEE2 antibody (AB121943)

Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labelling WEE2 with ab121943 at 2 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labeling WEE2 with ab121943 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human WEE2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P0C1S8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WEE2 also known as WEE kinase homolog 2 or oocyte-specific serine/threonine-protein kinase is a protein expressed mainly in oocytes. It has a mass of approximately 58 kDa. WEE2 functions as a cell cycle regulator that has a significant role in controlling the progression of cells through mitosis. The protein achieves this by phosphorylating and inactivating cyclin-dependent kinases (CDKs) specifically CDK1 which is necessary for cell cycle progression. This regulatory mechanism ensures proper timing and coordination during the cell cycle.
Biological function summary

The regulation of oocyte meiotic arrest and maturation involves WEE2. This protein is a part of regulatory complexes known to maintain oocytes in meiotic prophase arrest until hormonal cues signal resumption of the cell cycle. Proper function of WEE2 is vital for the maintenance of genomic integrity and viability of the oocyte. By controlling entry into M-phase WEE2 provides a safeguard against premature oocyte maturation ensuring that only developmentally ready oocytes proceed through meiosis.

Pathways

WEE2 plays significant roles in the mitosis and cell cycle control pathways. It is closely related to CDK1 and through its regulatory function influences the Cyclin B1/CDK1 complex that is critical for mitotic entry. WEE2 interacts with other pathways that involve cell cycle checkpoints ensuring that any DNA replication issues are addressed before mitosis proceeds. This interaction maintains genomic stability and prevents aneuploidy.

WEE2 has been linked to reproductive disorders such as female infertility specifically related to oocyte maturation defects. Mutations or dysregulation in WEE2 can impair its kinase activity affecting the proper progression of meiosis. Additionally improper WEE2 function may contribute to cancer development where cell cycle control is lost. WEE2 interacts with TP53 a protein important in cancer pathways further highlighting its significance in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II (PubMed : 29606300). Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).
See full target information WEE2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:129-168 PubMed39609633

2024

Bi-allelic variants in WDR47 cause a complex neurodevelopmental syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Efil Bayam,Peggy Tilly,Stephan C Collins,José Rivera Alvarez,Meghna Kannan,Lucile Tonneau,Elena Brivio,Bruno Rinaldi,Romain Lecat,Noémie Schwaller,Ludovica Cotellessa,Sateesh Maddirevula,Fabiola Monteiro,Carlos M Guardia,João Paulo Kitajima,Fernando Kok,Mitsuhiro Kato,Ahlam A A Hamed,Mustafa A Salih,Saeed Al Tala,Mais O Hashem,Hiroko Tada,Hirotomo Saitsu,Mariano Stabile,Paolo Giacobini,Sylvie Friant,Zafer Yüksel,Mitsuko Nakashima,Fowzan S Alkuraya,Binnaz Yalcin,Juliette D Godin

Clinical genetics 104:700-704 PubMed37772619

2023

Novel WEE2 homozygous mutations c.1346C>T and c.949A>T identified in primary infertile women due to unexplained fertilization failure.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Guo,Haibo Wu,Zheng Yan,Mingru Yin,Ling Wu,Bin Li

Journal of assisted reproduction and genetics 38:2861-2869 PubMed34476630

2021

Novel WEE2 compound heterozygous mutations identified in patients with fertilization failure or poor fertilization.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Jin,Xiaomei Tong,Yin-Li Zhang,Weijie Yang,Yerong Ma,Peipei Ren,Feng Zhou,Songying Zhang
View all publications
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Product promise

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