Rabbit Polyclonal Werner's syndrome helicase WRN antibody. Suitable for IHC-P and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human WRN aa 1-550.
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- Developmental cell 57:1347-1368.e122022Large-scale chromatin reorganization reactivates placenta-specific genes that drive cellular aging.Applications:WBReactive species:HumanZunpeng Liu,Qianzhao Ji,Jie Ren,Pengze Yan,Zeming Wu,Si Wang,Liang Sun,Zehua Wang,Jiaming Li,Guoqiang Sun,Chuqian Liang,Run Sun,Xiaoyu Jiang,Jianli Hu,Yingjie Ding,Qiaoran Wang,Shijia Bi,Gang Wei,Gang Cao,Guoguang Zhao,Hongmei Wang,Qi Zhou,Juan Carlos Izpisua Belmonte,Jing Qu,Weiqi Zhang,Guang-Hui LiuPubMed 35613614
- Aging 13:25717-257282021Calcification in Werner syndrome associated with lymphatic vessels aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHideyuki Ogata,Shinsuke Akita,Sanae Ikehara,Kazuhiko Azuma,Takashi Yamaguchi,Maihulan Maimaiti,Yoshiro Maezawa,Yoshitaka Kubota,Koutaro Yokote,Nobuyuki Mitsukawa,Yuzuru IkeharaPubMed 34958633
- Allergy, asthma & immunology research 12:507-5222020Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGwanghui Ryu,Jun Sang Bae,Ji Hye Kim,Eun Hee Kim,Lele Lyu,Young Jun Chung,Ji Hun MoPubMed 32141263
- Journal of diabetes research 2019:76024272019Metformin Reduces Lipotoxicity-Induced Meta-Inflammation in -Cells through the Activation of GPR40-PLC-IP3 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXimei Shen et. al.PubMed 31950065
- Cancer management and research 11:10011-100282019Simvastatin Evokes An Unpredicted Antagonism For Tamoxifen In MCF-7 Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmel B Ibrahim et. al.PubMed 31819634
- Scientific reports 9:100752019PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastian Veith et. al.PubMed 31296950
- DNA repair 79:22-312019Acetylation of Werner protein at K1127 and K1117 is important for nuclear trafficking and DNA repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeblina Ghosh et. al.PubMed 31085421
- The Journal of biological chemistry 294:1984-19962018The mitochondrial chaperone Prohibitin 1 negatively regulates interleukin-8 in human liver cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Won Yang,Ben Murray,Lucia Barbier-Torres,Ting Liu,Zhenqiu Liu,Heping Yang,Wei Fan,Jiaohong Wang,Yuan Li,Ekihiro Seki,José M Mato,Shelly C LuPubMed 30523154
- Cancer management and research 10:6649-66682018Distinct prognosis of mRNA expression of the five RecQ DNA-helicase family members - , , , , and - in patients with breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Zhu et. al.PubMed 30584360
- Scientific reports 8:148272018Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiaki Fujiwara et. al.PubMed 30287851
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WRN aa 1-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q14191
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
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Target data
Function
Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed:9224595, PubMed:9288107, PubMed:9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed:11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed:11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed:9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed:9224595, PubMed:9288107, PubMed:9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed:10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed:10212265). Depletion leads to chromosomal breaks and genome instability (PubMed:33199508).
Alternative names
RECQ3, RECQL2, WRN, Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, RecQ protein-like 2, Werner syndrome protein
Recommended products
Rabbit Polyclonal Werner's syndrome helicase WRN antibody. Suitable for IHC-P and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human WRN aa 1-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human WRN aa 1-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q14191
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Werner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.
- Biological function summary
WRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.
- Pathways
WRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.
- Associated diseases and disorders
WRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (ab200)
ab200 (4µg/ml) staining Werner's syndrome helicase WRN in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and some cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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