AB230851

Anti-Werner's syndrome helicase WRN antibody

Name: Anti-Werner's syndrome helicase WRN antibody (ab230851) | Abcam
Brand: Abcam Limited
SKU: AB230851
Price: 605 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Werner's syndrome helicase WRN antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WRN aa 750-850 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RECQ3, RECQL2, WRN, Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, RecQ protein-like 2, Werner syndrome protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (AB230851)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (AB230851)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Werner's syndrome helicase WRN using ab230851 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Werner's syndrome helicase WRN antibody (AB230851)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Werner's syndrome helicase WRN antibody (AB230851)

Intracellular flow cytometric analysis ofK562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Werner's syndrome helicase WRN with ab230851 at 1/10 dilution (green) compared with anegative control cell line (blue). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

Supplier Data

Western blot - Anti-Werner's syndrome helicase WRN antibody (AB230851)

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody (ab230851) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 35 µg

Predicted band size: 162 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human WRN aa 750-850 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q14191

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Werner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.

Biological function summary

WRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.

Pathways

WRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.

WRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed : 11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed : 11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed : 9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed : 10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed : 10212265). Depletion leads to chromosomal breaks and genome instability (PubMed : 33199508).

See full target information WRN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Protein and peptide letters 31:559-568 PubMed39076089

2024

MicroRNA-605-3p Inhibited the Growth and Chemoresistance of Osteosarcoma Cells via Negatively Modulating RAF1.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Wang,Weina Li,Guohui Han,Xiangdong Bai,Jun Xie

View all publications

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Product promise

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Anti-Werner's syndrome helicase WRN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (AB230851)

Flow Cytometry (Intracellular) - Anti-Werner's syndrome helicase WRN antibody (AB230851)

Western blot - Anti-Werner's syndrome helicase WRN antibody (AB230851)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise