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AB124673

Anti-Werner's syndrome helicase WRN antibody [EPR6392]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

4

(1 Review)

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(3 Publications)

Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) is a rabbit monoclonal antibody detecting Werner's syndrome helicase WRN in Western Blot. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

RECQ3, RECQL2, WRN, Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, RecQ protein-like 2, Werner syndrome protein

3 Images
Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)
  • WB

Lab

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 μg)
Lane 3 : MOLT4 cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 to 4 : Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)

Predicted band size: 162 kDa

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Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)
  • WB

Lab

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)

Lanes 1 - 4 : Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab124673 was shown to react with Werner's syndrome helicase WRN in wild-type HAP1 cells in western blot. Loss of signal was observed when WRN knockout sample was used. Wild-type and WRN knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124673 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

WRN knockout HAP1 cell lysate at 20 µg

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Predicted band size: 162 kDa

Observed band size: 170 kDa

false

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)
  • WB

Unknown

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

Lane 1:

MOLT4 cell lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

A431 cell lysates at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 162 kDa

false

  • Carrier free

    Anti-Werner's syndrome helicase WRN antibody [EPR6392] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6392

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Werner's syndrome helicase WRN?
Anti-Werner's syndrome helicase WRN [EPR6392] (ab124673) specifically detects a band for Werner's syndrome helicase WRN (UniProt: Q14191) at a molecular weight of 162kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) has been confirmed by Western blot testing in WRN Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR6392] also available for your convenience: ab124673, Carrier free - ab247955

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Werner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.
Biological function summary

WRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.

Pathways

WRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.

WRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed : 11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed : 11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed : 9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed : 10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed : 10212265). Depletion leads to chromosomal breaks and genome instability (PubMed : 33199508).
See full target information WRN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6059 PubMed39025847

2024

WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Rodríguez Pérez,Dean Natwick,Lauren Schiff,David McSwiggen,Alec Heckert,Melina Huey,Huntly Morrison,Mandy Loo,Rafael G Miranda,John Filbin,Jose Ortega,Kayla Van Buren,Danny Murnock,Arnold Tao,Renee Butler,Kylie Cheng,William Tarvestad,Zhengjian Zhang,Eric Gonzalez,Rand M Miller,Marcus Kelly,Yangzhong Tang,Jaclyn Ho,Daniel Anderson,Charlene Bashore,Stephen Basham

Scientific reports 13:645 PubMed36635307

2023

A non-catalytic N-terminus domain of WRN prevents mitotic telomere deprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Romero-Zamora,Makoto T Hayashi

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen
View all publications
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