AB176909

Anti-WFS1 antibody

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(2 Publications)

Rabbit Polyclonal WFS1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WFS1 aa 50-300.

View Alternative Names

Wolframin, WFS1

1 Images

Supplier Data

Western blot - Anti-WFS1 antibody (AB176909)

All lanes:

Western blot - Anti-WFS1 antibody (ab176909) at 1/500 dilution

Lane 1:

Human fetal heart lysate

Lane 2:

Human fetal muscle lysate

Predicted band size: 100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human WFS1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

O76024

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WFS1 also known as Wolfram Syndrome 1 protein is involved in localizing to the endoplasmic reticulum (ER) membrane. It consists of approximately 890 amino acids and has a molecular mass of about 100 kDa. It is mainly expressed in the brain pancreas and heart reflecting its structural role in cellular organelles. The protein has ER-luminal and cytoplasmic domains which implicate it in maintaining ER function and homeostasis.

Biological function summary

This protein plays a role in modulating ER stress responses and calcium homeostasis. It is a part of a signaling complex that influences apoptotic pathways by regulating cellular stress responses. WFS1 affects the stability and function of the ER having an influence over insulin production and secretion. This regulation is important for cells especially those in the pancreatic islets as it affects the secretion of insulin in response to varying glucose levels.

Pathways

WFS1 integrates into the unfolded protein response (UPR) and calcium signaling pathways. These pathways connect to other proteins like ATF6 and PERK which mediate stress-induced responses and adaptations in cells. In the context of insulin regulation the protein contributes to vesicular fusion mechanisms and secretory pathways that facilitate insulin release. WFS1 influences glucose metabolism through direct interactions in pathways regulating cellular stress and protein folding.

Mutations in this gene relate to Wolfram syndrome a condition characterized by diabetes insipidus diabetes mellitus optic atrophy and deafness. These mutations also connect to disorders like ataxia due to their involvement in neurodegenerative processes. WFS1 dysfunction impacts insulin regulation linking it with proteins like MANF that also mitigate ER stress and express protective roles against cellular stress conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store (PubMed : 16989814). Negatively regulates the ER stress response and positively regulates the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation through an unknown proteasome-independent mechanism (PubMed : 23035048).

See full target information WFS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5326 PubMed40527886

2025

Nuclear deformability increases PARPi sensitivity in BRCA1-deficient cells by increasing microtubule-dependent DNA break mobility.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Faustini,Angela Dello Stritto,Andrea Panza,Ylli Doksani,Francisca Lottersberger

Frontiers in neuroanatomy 16:779390 PubMed36003850

2022

Neurogliaform cells mediate feedback inhibition in the medial entorhinal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Szilard Szocs,Nora Henn-Mike,Agnes Agocs-Laboda,Edina Szabo-Meleg,Csaba Varga

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WFS1 antibody

Western blot - Anti-WFS1 antibody (AB176909)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise