AB230512

Anti-WFS1 antibody

Name: Anti-WFS1 antibody (ab230512) | Abcam
Brand: Abcam Limited
SKU: AB230512
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal WFS1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human WFS1 aa 150-250.

View Alternative Names

Wolframin, WFS1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WFS1 antibody (AB230512)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WFS1 antibody (AB230512)

Formalin-fixed, paraffin-embedded human testis tissue stained for WFS1 using ab230512 at 1 : 200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-WFS1 antibody (AB230512)

All lanes:

Western blot - Anti-WFS1 antibody (ab230512) at 1/500 dilution

All lanes:

Mouse brain extract

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human WFS1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O76024

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WFS1 also known as Wolfram Syndrome 1 protein is involved in localizing to the endoplasmic reticulum (ER) membrane. It consists of approximately 890 amino acids and has a molecular mass of about 100 kDa. It is mainly expressed in the brain pancreas and heart reflecting its structural role in cellular organelles. The protein has ER-luminal and cytoplasmic domains which implicate it in maintaining ER function and homeostasis.

Biological function summary

This protein plays a role in modulating ER stress responses and calcium homeostasis. It is a part of a signaling complex that influences apoptotic pathways by regulating cellular stress responses. WFS1 affects the stability and function of the ER having an influence over insulin production and secretion. This regulation is important for cells especially those in the pancreatic islets as it affects the secretion of insulin in response to varying glucose levels.

Pathways

WFS1 integrates into the unfolded protein response (UPR) and calcium signaling pathways. These pathways connect to other proteins like ATF6 and PERK which mediate stress-induced responses and adaptations in cells. In the context of insulin regulation the protein contributes to vesicular fusion mechanisms and secretory pathways that facilitate insulin release. WFS1 influences glucose metabolism through direct interactions in pathways regulating cellular stress and protein folding.

Mutations in this gene relate to Wolfram syndrome a condition characterized by diabetes insipidus diabetes mellitus optic atrophy and deafness. These mutations also connect to disorders like ataxia due to their involvement in neurodegenerative processes. WFS1 dysfunction impacts insulin regulation linking it with proteins like MANF that also mitigate ER stress and express protective roles against cellular stress conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store (PubMed : 16989814). Negatively regulates the ER stress response and positively regulates the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation through an unknown proteasome-independent mechanism (PubMed : 23035048).

See full target information WFS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:62 PubMed36890518

2023

Cannabinoids modulate the microbiota-gut-brain axis in HIV/SIV infection by reducing neuroinflammation and dysbiosis while concurrently elevating endocannabinoid and indole-3-propionate levels.

Applications

Unspecified application

Species

Unspecified reactive species

Marina McDew-White,Eunhee Lee,Lakmini S Premadasa,Xavier Alvarez,Chioma M Okeoma,Mahesh Mohan

Cellular signalling 48:69-80 PubMed29753111

2018

IRAK2 counterbalances oncogenic Smurf1 in colon cancer cells by dictating ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Liu,Yuhan Chen,Qingyang Huang,Wen Liu,Xiqing Ji,Fan Hu,Ying Zhu,Lingqiang Zhang,Guanglong Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WFS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WFS1 antibody (AB230512)

Western blot - Anti-WFS1 antibody (AB230512)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise