Mouse Monoclonal WHSC1/NSD2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 39 publications.
Images
![Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--western-blot-img151989.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)")
![Immunoprecipitation - Anti-WHSC1/NSD2 antibody [29D1] (AB75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--immunoprecipitation-img146547.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)")
![Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--western-blot-img16955.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)")
![Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--western-blot-img96324.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)")
Publications
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- Haematologica 109:1893-19082024Epigenetic dysregulation of eukaryotic initiation factor 3 subunit E (eIF3E) by lysine methyltransferase REIIBP confers a pro-inflammatory phenotype in t(4;14) myeloma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhyllis S Y Chong,Jing Yuan Chooi,Sze Lynn Julia Lim,Tae-Hoon Chung,Reinhard Brunmeir,Aaron Chung Yong Leow,Sabrina Hui Min Toh,Kalpnaa Balan,Muhamad Irfan Bin Azaman,Zhengwei Wu,Nagavidya Subramaniam,Leah A Vardy,Wee-Joo ChngPubMed 38124661
- Scientific reports 13:216292023Association of WHSC1/NSD2 and T-cell infiltration with prostate cancer metastasis and prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiheng Li,Jiang Zhu,Yang Zhang,Yun Pan,Zhengjin Li,Min Wang,Yixuan Gao,Dongmei Feng,Xiaoyong He,Chunmei ZhangPubMed 38062230
- The Tohoku journal of experimental medicine 260:193-2042023Tumor Promoting Role of NRIP1 in Oral Squamous Cell Carcinoma: The Involvement of NSD2-Mediated Histone Methylation of DGCR8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Zhang,Guangyao HuPubMed 37045786
- Journal of medicinal chemistry 65:10611-106252022Discovery of a First-in-Class Degrader for Nuclear Receptor Binding SET Domain Protein 2 (NSD2) and Ikaros/Aiolos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanye Meng,Chenxi Xu,Kwang-Su Park,H Ümit Kaniskan,Gang Greg Wang,Jian JinPubMed 35895319
- Acta pharmaceutica Sinica. B 12:1871-18842022Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop NRF2 in cancer therapeutic resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Wang,Qianqian Wang,Guodi Cai,Zhijian Duan,Zoann Nugent,Jie Huang,Jianwei Zheng,Alexander D Borowsky,Jian Jian Li,Peiqing Liu,Hsing-Jien Kung,Leigh Murphy,Hong-Wu Chen,Junjian WangPubMed 35847493
- Molecular cell 82:2472-2489.e82022NSD1 mediates antagonism between SWI/SNF and polycomb complexes and is required for transcriptional activation upon EZH2 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiannis Drosos,Jacquelyn A Myers,Beisi Xu,Kaeli M Mathias,Emma C Beane,Sandi Radko-Juettner,Robert J Mobley,Margaret E Larsen,Federica Piccioni,Xiaotu Ma,Jonathan Low,Baranda S Hansen,Samuel T Peters,Natarajan V Bhanu,Sandeep K Dhanda,Taosheng Chen,Santhosh A Upadhyaya,Shondra M Pruett-Miller,David E Root,Benjamin A Garcia,Janet F Partridge,Charles W M RobertsPubMed 35537449
- The Journal of clinical investigation 132:2022Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiale Ren,Ni Li,Siyu Pei,Yannan Lian,Li Li,Yuchong Peng,Qiuli Liu,Jiacheng Guo,Xuege Wang,Ying Han,Guoying Zhang,Hanling Wang,Yaqi Li,Jun Jiang,Qintong Li,Minjia Tan,Junjie Peng,Guohong Hu,Yichuan Xiao,Xiong Li,Moubin Lin,Jun QinPubMed 35230972
- Frontiers in immunology 12:7514092022PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNianyin Lv,Sufeng Jin,Zihao Liang,Xiaohui Wu,Yanhua Kang,Lan Su,Yeping Dong,Bingwei Wang,Tonghui Ma,Liyun ShiPubMed 35069527
- Nature chemical biology 18:56-632021A chemical probe targeting the PWWP domain alters NSD2 nucleolar localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Dilworth,Ronan P Hanley,Renato Ferreira de Freitas,Abdellah Allali-Hassani,Mengqi Zhou,Naimee Mehta,Matthew R Marunde,Suzanne Ackloo,Raquel Arminda Carvalho Machado,Aliakbar Khalili Yazdi,Dominic D G Owens,Victoria Vu,David Y Nie,Mona Alqazzaz,Edyta Marcon,Fengling Li,Irene Chau,Albina Bolotokova,Su Qin,Ming Lei,Yanli Liu,Magdalena M Szewczyk,Aiping Dong,Sina Kazemzadeh,Tigran Abramyan,Irina K Popova,Nathan W Hall,Matthew J Meiners,Marcus A Cheek,Elisa Gibson,Dmitri Kireev,Jack F Greenblatt,Michael-C Keogh,Jinrong Min,Peter J Brown,Masoud Vedadi,Cheryl H Arrowsmith,Dalia Barsyte-Lovejoy,Lindsey I James,Matthieu SchapiraPubMed 34782742
- Frontiers in oncology 10:6005142021Overexpression of EZH2/NSD2 Histone Methyltransferase Axis Predicts Poor Prognosis and Accelerates Tumor Progression in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Gao,Xiumin Liu,Zhengjin Li,Lixian Zhao,Yun PanPubMed 33665162
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
1 product for Alternative Version
Target data
Function
Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed:19808676, PubMed:22099308, PubMed:27571355, PubMed:29728617, PubMed:33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed:22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed:22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed:16115125, PubMed:22099308, PubMed:29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed:29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity). Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2). Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed:22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed:18172012, PubMed:22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed:18172012). Does not methylate histone H3 at 'Lys-27' (PubMed:22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed:11152655, PubMed:18172012).
Alternative names
KIAA1090, MMSET, TRX5, WHSC1, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein
Recommended products
Mouse Monoclonal WHSC1/NSD2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 39 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 29D1
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.
- Biological function summary
WHSC1/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1/NSD2 plays a significant role in development and cellular responses to environmental signals.
- Pathways
WHSC1/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.
- Associated diseases and disorders
Alterations in WHSC1/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
![Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--western-blot-img151989.jpg "Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)")
Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
Lanes 1- 2: Merged signal (red and green). Green - ab75359 observed at 80 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) observed at 37 kDa.ab75359 was shown to react with WHSC1/NSD2 in wild-type HeLa cells in western blot. The band observed in knockout cell line Human WHSC1 (NSD2) knockout HeLa cell line ab264936 (knockout cell lysate Human WHSC1 (NSD2) knockout HeLa cell lysate ab257798) lane below 80kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and WHSC1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75359 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at a 5 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: WHSC1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 80 kDa
![Immunoprecipitation - Anti-WHSC1/NSD2 antibody [29D1] (ab75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--immunoprecipitation-img146547.jpg "Immunoprecipitation - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)")
Immunoprecipitation - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
WHSC1/NSD2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 10µg of Mouse monoclonal to WHSC1/NSD2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75359.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 71kDa: WHSC1/NSD2.All lanes: Immunoprecipitation - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
Predicted band size: 152 kDa
![Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--western-blot-img16955.jpg "Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)")
Image courtesy of an anonymous customer review.Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
Blocked with 5% milk for 1 hour at 23°C.
All lanes: Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359) at 1/5000 dilution
Lane 1: Whole cell lysate prepared from HeLa cells at 50 µg
Lane 2: Whole cell lysate prepared from HCT116 cells at 50 µg
SecondaryAll lanes: HRP conjugated goat anti-mouse polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 152 kDa
Exposure time: 3min
![Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359), expandable thumbnail](https://content.abcam.com/products/images/whsc1-nsd2-antibody-29d1-ab75359--western-blot-img96324.jpg "Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)")
Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
The full-length MMSET-II protein has an apparent molecular weight of approximately 180 kDa, whereas the 645 amino acid long MMSET-I exhibits a mobility of approximately 80 kDa.
Abcam recommends blocking with 1% milk.
All lanes: Western blot - Anti-WHSC1/NSD2 antibody [29D1] (ab75359) at 5 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 180 kDa, 80 kDa
Exposure time: 20min
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