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AB223694

Anti-WHSC1/NSD2 antibody

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(2 Publications)

Rabbit Polyclonal WHSC1/NSD2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSD2 aa 1-150.

View Alternative Names

KIAA1090, MMSET, TRX5, WHSC1, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHSC1/NSD2 antibody (AB223694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHSC1/NSD2 antibody (AB223694)

Paraffin-embedded human oral mucosa tissue stained for WHSC1/NSD2 using ab223694 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-WHSC1/NSD2 antibody (AB223694)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WHSC1/NSD2 antibody (AB223694)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for WHSC1/NSD2 (green) using ab223694 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NSD2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O96028

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.
Biological function summary

WHSC1/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1/NSD2 plays a significant role in development and cellular responses to environmental signals.

Pathways

WHSC1/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.

Alterations in WHSC1/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 19808676, PubMed : 22099308, PubMed : 27571355, PubMed : 29728617, PubMed : 33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed : 22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed : 22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed : 16115125, PubMed : 22099308, PubMed : 29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed : 29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity).. Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2).. Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed : 18172012, PubMed : 22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed : 18172012). Does not methylate histone H3 at 'Lys-27' (PubMed : 22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed : 11152655, PubMed : 18172012).
See full target information NSD2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Innate immunity 27:437-447 PubMed34428935

2021

Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Liu,Benlong Cai,Dan Li,Yuan Yao

OncoTargets and therapy 13:7033-7044 PubMed32801739

2020

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Dai,Longfeng Jiang,Lei Qiu,Yuyun Shao,Ping Shi,Jun Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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