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AB317010

Anti-WHSC1/NSD2 antibody [EPR23777-95]

  • Advanced Validation
  • 20ul selling size
  • Recombinant
  • RabMAb
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Rabbit Recombinant Monoclonal WHSC1/NSD2 antibody. Suitable for WB, IP, ChIP-seq and reacts with Human samples.

View Alternative Names

KIAA1090, MMSET, TRX5, WHSC1, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein

7 Images
Immunoprecipitation - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • IP

Supplier Data

Immunoprecipitation - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

WHSC1/NSD2 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab317010 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317010 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab317010 IP in K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317010 in K562 whole cell lysate

All lanes:

Immunoprecipitation - Anti-WHSC1/NSD2 antibody [EPR23777-95] (ab317010) at 1/30 dilution

All lanes:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 93s

ChIP-sequencing - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

Chromatin was prepared from K-562 cells. Cells were fixed with 1.5 mM EGS for 30 min combined with 1% formaldehyde for 10 min. ChIP was performed with 107 cells and 8 μg of ab317010 [EPR23777-95]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

The Input control is also shown.

ChIP-sequencing - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

Chromatin was prepared from K-562 cells. Cells were fixed with 1.5 mM EGS for 30 min combined with 1% formaldehyde for 10 min. ChIP was performed with 107 cells and 8 μg of ab317010 [EPR23777-95]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

The Input control is also shown.

Immunoprecipitation - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • IP

Supplier Data

Immunoprecipitation - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

WHSC1/NSD2 was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate with ab317010 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317010 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 2 : ab317010 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317010 in MOLT-4 whole cell lysate

All lanes:

Immunoprecipitation - Anti-WHSC1/NSD2 antibody [EPR23777-95] (ab317010) at 1/30 dilution

All lanes:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

ChIP-sequencing - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

Chromatin was prepared from K-562 cells. Cells were fixed with 1.5 mM EGS for 30 min combined with 1% formaldehyde for 10 min. ChIP was performed with 107 cells and 8 μg of ab317010 [EPR23777-95]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

The Input control is also shown.

Western blot - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • WB

Supplier Data

Western blot - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

Lane1 was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-WHSC1/NSD2 antibody [EPR23777-95] (ab317010) at 1/1000 dilution

All lanes:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 152 kDa

false

Exposure time: 37s

Western blot - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)
  • WB

Supplier Data

Western blot - Anti-WHSC1/NSD2 antibody [EPR23777-95] (AB317010)

Lane 1and 2 were freshly made and used for Western blotting immediately to minimize protein degradation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-WHSC1/NSD2 antibody [EPR23777-95] (ab317010) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 4:

HeLa transfected with siRNA specifically targeting NDS2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 152 kDa,124 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-WHSC1/NSD2 antibody [EPR23777-95] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23777-95

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ChIP-seq

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "8 µg", "ChIPseq-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.
Biological function summary

WHSC1/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1/NSD2 plays a significant role in development and cellular responses to environmental signals.

Pathways

WHSC1/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.

Alterations in WHSC1/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 19808676, PubMed : 22099308, PubMed : 27571355, PubMed : 29728617, PubMed : 33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed : 22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed : 22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed : 16115125, PubMed : 22099308, PubMed : 29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed : 29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity).. Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2).. Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed : 18172012, PubMed : 22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed : 18172012). Does not methylate histone H3 at 'Lys-27' (PubMed : 22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed : 11152655, PubMed : 18172012).
See full target information NSD2
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Product promise

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