AB167675

Anti-WHSC2/NELF-A antibody

Name: Anti-WHSC2/NELF-A antibody (ab167675) | Abcam
Brand: Abcam Limited
SKU: AB167675
Price: 545 USD
Availability: InStock

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(2 Publications)

Mouse Polyclonal WHSC2/NELF-A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NELFA.

View Alternative Names

WHSC2, P/OKcl.15, NELFA, Negative elongation factor A, NELF-A, Wolf-Hirschhorn syndrome candidate 2 protein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-WHSC2/NELF-A antibody (AB167675)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WHSC2/NELF-A antibody (AB167675)

Immunofluorescence analysis of HeLa cells, labeling WHSC2/NELF-A with ab167675 at 10 μg/ml.

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Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

All lanes:

Western blot - Anti-WHSC2/NELF-A antibody (ab167675) at 1/500 dilution

All lanes:

NIH/3T3 cell lysate at 50 µg

Predicted band size: 57 kDa

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Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

All lanes:

Western blot - Anti-WHSC2/NELF-A antibody (ab167675) at 1 µg/mL

Lane 1:

WHSC2/NELF-A transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 57 kDa

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Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

All lanes:

Western blot - Anti-WHSC2/NELF-A antibody (ab167675) at 1/500 dilution

All lanes:

Raw 264.7 cell lysate at 50 µg

Predicted band size: 57 kDa

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Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

All lanes:

Western blot - Anti-WHSC2/NELF-A antibody (ab167675) at 1/500 dilution

All lanes:

HeLa cell nuclear extract at 50 µg

Predicted band size: 57 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NELFA.

Q9H3P2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: 99% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WHSC2 also known as NELF-A mechanically operates as a transcription elongation factor. It is a 66 kDa protein expressed in various tissues throughout the human body with higher levels in the brain and testis. WHSC2 is part of the negative elongation factor (NELF) complex. This complex interacts with RNA polymerase II (RNAPII) to pause transcription elongation an important step in regulating gene expression.

Biological function summary

WHSC2 plays a significant role in maintaining transcriptional pausing which affects gene expression. It is a component of the NELF complex which includes NELF-B NELF-C/D and NELF-E. By pausing RNAPII WHSC2 regulates the timing of transcription and impacts processes like differentiation and cell cycle control. This function is important for responding to environmental signals and cellular requirements.

Pathways

WHSC2 participates in the transcription regulation pathway and the cell cycle control pathway. It is related to proteins such as DSIF which also influences RNA polymerase II pausing. By being part of these pathways WHSC2 exerts control over the transition from transcription initiation to elongation impacting the expression timing of many genes. This positioning allows it to coordinate with other regulatory proteins and respond to cellular changes.

WHSC2 links to Wolf-Hirschhorn syndrome and various cancers. In Wolf-Hirschhorn syndrome deletions affecting WHSC2 contribute to developmental delays and growth retardation. In cancer altered WHSC2 expression is associated with abnormal cell growth particularly through interactions with p53 a tumor suppressor protein. The relationship highlights the importance of WHSC2 in both genetic disorders and oncogenic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

See full target information NELFA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111865 PubMed36577375

2022

Distinct patterns of RNA polymerase II and transcriptional elongation characterize mammalian genome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichiro Abe,Tamas Schauer,Maria-Elena Torres-Padilla

The Journal of biological chemistry 292:9063-9074 PubMed28364042

2017

Kinetic-based trapping by intervening sequence variants of the active sites of protein-disulfide isomerase identifies platelet protein substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Jack D Stopa,Katherine M Baker,Steven P Grover,Robert Flaumenhaft,Bruce Furie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WHSC2/NELF-A antibody

Immunocytochemistry/ Immunofluorescence - Anti-WHSC2/NELF-A antibody (AB167675)

Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

Western blot - Anti-WHSC2/NELF-A antibody (AB167675)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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