AB9377

Anti-wide spectrum Cytokeratin antibody

Name: Wide spectrum cytokeratin antibody - rabbit polyclonal (ab9377) | Abcam
Brand: Abcam Limited
SKU: AB9377
Price: 400 USD
Availability: InStock
Rating: 5 (43 reviews)

Lab Essentials
What is this?

(43 Reviews)

(215 Publications)

Anti-wide spectrum Cytokeratin antibody (ab9377) is a rabbit polyclonal antibody detecting wide spectrum Cytokeratin in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Common marmoset, Cow, Monkey, Dog, Human, Mouse, Pig, Rat.

- Over 170 publications
- Trusted since 2002

View Alternative Names

Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5, CK5, Cytokeratin VIB, Cytokeratin-10, Cytokeratin-6B, Keratin-10, CK-10, K10, KRT10, Cytokeratin VII, Cytokeratin-14, Cytokeratin-7, Keratin-14, CK-14, K14, KRT14, Cytokeratin-17, Keratin-17, CK-17, K17, KRT17

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

IHC-P

AbReview48363****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

ab9377 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor^® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

Image is courtesy of an anonymous AbReview

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.

IHC-P

AbReview79886****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 10% formalin-fixed Human transitional cell carcinoma of the kidney. Stained with ab9377 at 1/100 dilution. Secondary antibody used was goat anti-rabbit Alexa-568^® at 1/500 dilution. Blocking was done with 5% serum for 1 hour at 25°C. The sample was incubated with the primary antibody for 1 hour at 25°C with 5% normal goat serum. Antigen retrieval method was heat mediated with EDTA pH 8.

This image is courtesy of an Abreview submitted by Cameron Johnstone.

IHC-P

AbReview56113****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

This image is courtesy of an Abreview by Carl Hobbs.

IHC-P

AbReview3439****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.

IHC-P

AbReview56111****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

This image is courtesy of an Abreview by Carl Hobbs.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human, Mouse, Cow, Pig, Dog, Cynomolgus monkey, Rat, Common marmoset

Applications

Flow Cyt, ICC, WB, IHC-Fr, ICC/IF, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Cow KRT5.

Q5XQN5

Specificity

This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "This antibody may be diluted to a titer of 1:75-1:200 in an ABC method." }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Product Specifications
Anti-wide spectrum Cytokeratin antibody (ab9377) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB in common marmoset, cow, cynomolgus monkey, dog, human, mouse, pig, rat samples.
Anti-wide spectrum Cytokeratin antibody (ab9377) specifically detects wide spectrum Cytokeratin (UniProt ID: ; Molecular weight: kDa) and is sold in 500 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-wide spectrum Cytokeratin antibody (ab9377) has high sensitivity and specificity.
Anti-wide spectrum Cytokeratin antibody (ab9377) has been cited over 174 times in peer reviewed journals and is trusted by the scientific community.
Anti-wide spectrum Cytokeratin antibody (ab9377) has 43 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

See full target information KRT5

Additional targets

Keratin, type I cytoskeletal 14,KRT17,

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Publications (215)

Recent publications for all applications. Explore the full list and refine your search

Bone research 13:83 PubMed41038809

2025

Injured bone-triggered osteokines secretion promotes diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Shen,Kai Dai,Shuang Zhang,Jing Wang,Changsheng Liu

Scientific reports 15:33996 PubMed41028058

2025

DUSP6 is upregulated in metastasis and influences migration and metabolism in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana T Ruckert,R McKinnon Walsh,Bailey A Bye,Austin E Eades,Wei Yan,Filip Bednar,Jiaqi Shi,Emily L Lasse Opsahl,Marina Pasca di Magliano,Costas A Lyssiotis,Michael N VanSaun,Vanessa S Silveira

Journal of experimental & clinical cancer research : CR 44:238 PubMed40813707

2025

HAS1 cancer associated fibroblasts located at the tumor invasion front zone promote oral squamous cell carcinoma invasion via ECM remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Wanyong Jin,Qiuya Yu,Liyuan Yu,Ting Zhou,Xiren Wang,Wanqiu Lu,Xiaoxin Zhang,Liang Ding,Qingang Hu,Yanhong Ni

Scientific reports 15:29306 PubMed40790149

2025

Target sequence of single cells captured by a polymeric microfluidic device.

Applications

Unspecified application

Species

Unspecified reactive species

Rintaro Oyama,Masataka Mori,Hiroki Matsumiya,Yukiko Nemoto,Natsumasa Nishizawa,Yohei Honda,Masatoshi Kanayama,Akihiro Taira,Taiji Kuwata,Masaru Takenaka,Koji Kuroda,Kazue Yoneda,Takashi Ohnaga,Motoyoshi Endo,Fumihiro Tanaka

Cancers 17: PubMed40723173

2025

Establishing a Highly Accurate Circulating Tumor Cell Image Recognition System for Human Lung Cancer by Pre-Training on Lung Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Matsumiya,Kenji Terabayashi,Yusuke Kishi,Yuki Yoshino,Masataka Mori,Masatoshi Kanayama,Rintaro Oyama,Yukiko Nemoto,Natsumasa Nishizawa,Yohei Honda,Taiji Kuwata,Masaru Takenaka,Yasuhiro Chikaishi,Kazue Yoneda,Koji Kuroda,Takashi Ohnaga,Tohru Sasaki,Fumihiro Tanaka

Nature communications 16:6578 PubMed40675962

2025

The tumor-sentinel lymph node immuno-migratome reveals CCR7⁺ dendritic cells drive response to sequenced immunoradiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Saddawi-Konefka,Riyam Al Msari,Shiqi Tang,Chad Philips,Sayed Sadat,Lauren M Clubb,Sarah Luna,Santiago Fassardi,Riley Jones,Ida Franiak Pietryga,Farhoud Faraji,Shiruyeh Schokrpur,Bryan S Yung,Michael M Allevato,Kelsey E Decker,Chanond A Nasamran,Daisy Chilin-Fuentes,Sara Brin Rosenthal,Shawn M Jensen,Bernard A Fox,R Bryan Bell,J Silvio Gutkind,Andrew Sharabi,Joseph A Califano

Biological procedures online 27:21 PubMed40524168

2025

Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening.

Applications

Unspecified application

Species

Unspecified reactive species

Yajing Liu,Jingyuan Ning,Donglai Wang

PloS one 20:e0320134 PubMed40245075

2025

Temporal effect of docetaxel on bone quality in a rodent model of vertebral metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Margarete K Akens,Mohammedayaz Rangrez,Allison Tolgyesi,Thomas L Willett,Cari M Whyne

Journal of tissue engineering and regenerative medicine 2024:2515383 PubMed40225757

2025

Polycaprolactone Fiber and Laminin and Collagen IV Protein Incorporation in Implants Enhances Wound Healing in a Novel Mouse Skin Splint Model.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Gadalla,Maeve Kennedy,Jamie Ganem,Mustafa Suppah,Alessandra Schmitt,David G Lott

Nature communications 16:1751 PubMed39966435

2025

A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Rana El Masri,Alberto Iannuzzo,Paul Kuentz,Rachida Tacine,Marie Vincent,Sébastien Barbarot,Fanny Morice-Picard,Franck Boralevi,Naia Oillarburu,Juliette Mazereeuw-Hautier,Yannis Duffourd,Laurence Faivre,Arthur Sorlin,Pierre Vabres,Jérôme Delon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-wide spectrum Cytokeratin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (AB9377)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (215)

Product promise