Anti-wide spectrum cytokeratin rabbit polyclonal antibody that is used to detect wide spectrum cytokeratin in Western Blot, IHC, ICC/IF and Flow Cytometry. Suitable for Human, Mouse, Rat samples and more.
- This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa
- Cited in over 170 publications
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Publications
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- In vivo (Athens, Greece) 38:226-2342024Decreased SMP30 Expression Is Related With EMT in the Kidneys of Two Siberian Tigers With CKD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Rang Jung,Jae-Hyuk Yim,Young-Jin Lee,Sae-Bom Lee,Sung-Yong Heo,Seul-Gi Bae,Kyoo-Tae Kim,Young-Sam Kwon,Sang-Joon Park,Jin-Kyu Park,Tae-Hwan KimPubMed 38148068
- Nature 623:803-8132023Autoantibodies against type I IFNs in humans with alternative NF-κB pathway deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTom Le Voyer,Audrey V Parent,Xian Liu,Axel Cederholm,Adrian Gervais,Jérémie Rosain,Tina Nguyen,Malena Perez Lorenzo,Elze Rackaityte,Darawan Rinchai,Peng Zhang,Lucy Bizien,Gonca Hancioglu,Pascale Ghillani-Dalbin,Jean-Luc Charuel,Quentin Philippot,Mame Sokhna Gueye,Majistor Raj Luxman Maglorius Renkilaraj,Masato Ogishi,Camille Soudée,Mélanie Migaud,Flore Rozenberg,Mana Momenilandi,Quentin Riller,Luisa Imberti,Ottavia M Delmonte,Gabriele Müller,Baerbel Keller,Julio Orrego,William Alexander Franco Gallego,Tamar Rubin,Melike Emiroglu,Nima Parvaneh,Daniel Eriksson,Maribel Aranda-Guillen,David I Berrios,Linda Vong,Constance H Katelaris,Peter Mustillo,Johannes Raedler,Jonathan Bohlen,Jale Bengi Celik,Camila Astudillo,Sarah Winter,Catriona McLean,Aurélien Guffroy,Joseph L DeRisi,David Yu,Corey Miller,Yi Feng,Audrey Guichard,Vivien Béziat,Jacinta Bustamante,Qiang Pan-Hammarström,Yu Zhang,Lindsey B Rosen,Steve M Holland,Marita Bosticardo,Heather Kenney,Riccardo Castagnoli,Charlotte A Slade,Kaan Boztuğ,Nizar Mahlaoui,Sylvain Latour,Roshini S Abraham,Vassilios Lougaris,Fabian Hauck,Anna Sediva,Faranaz Atschekzei,Georgios Sogkas,M Cecilia Poli,Mary A Slatter,Boaz Palterer,Michael D Keller,Alberto Pinzon-Charry,Anna Sullivan,Luke Droney,Daniel Suan,Melanie Wong,Alisa Kane,Hannah Hu,Cindy Ma,Hana Grombiříková,Peter Ciznar,Ilan Dalal,Nathalie Aladjidi,Miguel Hie,Estibaliz Lazaro,Jose Franco,Sevgi Keles,Marion Malphettes,Marlene Pasquet,Maria Elena Maccari,Andrea Meinhardt,Aydan Ikinciogullari,Mohammad Shahrooei,Fatih Celmeli,Patrick Frosk,Christopher C Goodnow,Paul E Gray,Alexandre Belot,Hye Sun Kuehn,Sergio D Rosenzweig,Makoto Miyara,Francesco Licciardi,Amélie Servettaz,Vincent Barlogis,Guillaume Le Guenno,Vera-Maria Herrmann,Taco Kuijpers,Grégoire Ducoux,Françoise Sarrot-Reynauld,Catharina Schuetz,Charlotte Cunningham-Rundles,Frédéric Rieux-Laucat,Stuart G Tangye,Cristina Sobacchi,Rainer Doffinger,Klaus Warnatz,Bodo Grimbacher,Claire Fieschi,Laureline Berteloot,Vanessa L Bryant,Sophie Trouillet Assant,Helen Su,Benedicte Neven,Laurent Abel,Qian Zhang,Bertrand Boisson,Aurélie Cobat,Emmanuelle Jouanguy,Olle Kampe,Paul Bastard,Chaim M Roifman,Nils Landegren,Luigi D Notarangelo,Mark S Anderson,Jean-Laurent Casanova,Anne PuelPubMed 37938781
- Cancers 15:2023Glucocorticoid Receptor Activation in Lobular Breast Cancer Is Associated with Reduced Cell Proliferation and Promotion of Metastases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaylee A Porter,Candace Frerich,Muriel Lainé,Abigail B Clark,Ishrat Durdana,Jeon Lee,Manisha Taya,Sunati Sahoo,Geoffrey L Greene,Lynda Bennett,Suzanne D ConzenPubMed 37835373
- JCI insight 8:2023CCL24 regulates biliary inflammation and fibrosis in primary sclerosing cholangitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaanan Greenman,Michal Segal-Salto,Neta Barashi,Ophir Hay,Avi Katav,Omer Levi,Ilan Vaknin,Revital Aricha,Sarit Aharoni,Tom Snir,Inbal Mishalian,Devorah Olam,Johnny Amer,Ahmad Salhab,Rifaat Safadi,Yaakov Maor,Palak Trivedi,Christopher J Weston,Francesca Saffioti,Andrew Hall,Massimo Pinzani,Douglas Thorburn,Amnon Peled,Adi MorPubMed 37345655
- BMC cancer 23:4362023Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Dietsch,Céline Weller,Carsten Sticht,Carolina de la Torre,Martin Kramer,Sergij Goerdt,Cyrill Géraud,Sebastian A WohlfeilPubMed 37179302
- STAR protocols 4:1022432023Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnxo Martinez-Ordoñez,Tania Cid-Diaz,Angeles Duran,Qixiu Han,Jorge Moscat,Maria T Diaz-MecoPubMed 37083323
- Biomolecules 13:2022First Evidence of the Protective Effects of 2-Pentadecyl-2-Oxazoline (PEA-OXA) in In Vitro Models of Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAniello Schiano Moriello,Fiorentina Roviezzo,Fabio Arturo Iannotti,Giuseppina Rea,Marco Allarà,Rosa Camerlingo,Roberta Verde,Vincenzo Di Marzo,Stefania PetrosinoPubMed 36671418
- Life science alliance 6:2022Loss of cell-cell adhesion triggers cell migration through Rac1-dependent ROS generation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Hsuan Chen,Jinn-Yuan Hsu,Ching-Tung Chu,Yao-Wen Chang,Jia-Rong Fan,Muh-Hwa Yang,Hong-Chen ChenPubMed 36446524
- Cell death & disease 13:8412022GSDME deficiency leads to the aggravation of UVB-induced skin inflammation through enhancing recruitment and activation of neutrophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujie Chen,Ni Lian,Sihan Chen,Ta Xiao,Yangying Ke,Yiqun Zhang,Changjun Song,Yong Yang,Song Xu,Heng Gu,Xu ChenPubMed 36182937
- Evolution, medicine, and public health 10:447-4622022Evolution of higher mesenchymal CD44 expression in the human lineage: A gene linked to cancer malignancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinghong Ma,Anasuya Dighe,Jamie Maziarz,Edwin Neumann,Eric Erkenbrack,Yuan-Yuan Hei,Yansheng Liu,Yasir Suhail,Irene Pak,Andre Levchenko,Günter P WagnerPubMed 36148042
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.1% Sodium azide
Constituents: 10% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Cow KRT5. Database link Q5XQN5
Consider this alternative
Reactivity data
Select an application| ICC | Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Expected | Tested |
| Mouse | Expected | Expected | Expected | Expected | Expected | Predicted |
| Rat | Expected | Expected | Expected | Expected | Expected | Predicted |
| Common marmoset | Expected | Expected | Expected | Expected | Expected | Predicted |
| Cow | Expected | Expected | Expected | Expected | Expected | Predicted |
| Cynomolgus monkey | Expected | Expected | Expected | Expected | Expected | Predicted |
| Dog | Expected | Expected | Expected | Expected | Expected | Predicted |
| Pig | Expected | Expected | Expected | Expected | Expected | Predicted |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Common marmoset | Dilution info - | Notes - |
Species Cynomolgus monkey | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Pig | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Common marmoset | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cynomolgus monkey | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Dog | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Rat | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Pig | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Common marmoset | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Cynomolgus monkey | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Human | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Dog | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
Species Cow | Dilution info - | Notes Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Common marmoset, Cynomolgus monkey, Human, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Rat | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Pig | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Common marmoset | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Cynomolgus monkey | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Human | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Dog | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Species Cow | Dilution info - | Notes This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig, Dog, Cynomolgus monkey, Rat, Common marmoset | Dilution info - | Notes - |
Target data
Function
Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Additional Targets
Keratin, type I cytoskeletal 14, KRT17, Keratin, type I cytoskeletal 10
Alternative names
Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5
Recommended products
Anti-wide spectrum cytokeratin rabbit polyclonal antibody that is used to detect wide spectrum cytokeratin in Western Blot, IHC, ICC/IF and Flow Cytometry. Suitable for Human, Mouse, Rat samples and more.
- This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa
- Cited in over 170 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.1% Sodium azide
Constituents: 10% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Cow KRT5. Database link Q5XQN5
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa.
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-wide spectrum Cytokeratin antibody (ab9377) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB in common marmoset, cow, cynomolgus monkey, dog, human, mouse, pig, rat samples.
Anti-wide spectrum Cytokeratin antibody (ab9377) specifically detects wide spectrum Cytokeratin (UniProt ID: ; Molecular weight: kDa) and is sold in 500 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-wide spectrum Cytokeratin antibody (ab9377) has high sensitivity and specificity.
Anti-wide spectrum Cytokeratin antibody (ab9377) has been cited over 174 times in peer reviewed journals and is trusted by the scientific community.
Anti-wide spectrum Cytokeratin antibody (ab9377) has 43 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
ab9377 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.
This image is courtesy of a customer review by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
This image is courtesy of a customer review by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
This image is courtesy of a customer review submitted by Cameron Johnstone.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 10% formalin-fixed Human transitional cell carcinoma of the kidney. Stained with ab9377 at 1/100 dilution. Secondary antibody used was goat anti-rabbit Alexa-568® at 1/500 dilution. Blocking was done with 5% serum for 1 hour at 25°C. The sample was incubated with the primary antibody for 1 hour at 25°C with 5% normal goat serum. Antigen retrieval method was heat mediated with EDTA pH 8.
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