Rabbit Polyclonal WIF1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WIF1.
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Publications
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- BioFactors (Oxford, England) 48:683-6982022Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen-induced retinopathy mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Tan,Huizhuo Xu,Bolin Chen,Tianqi Duan,Kangcheng Liu,Jing ZouPubMed 35080047
- Nature communications 11:9912020Tracing tumorigenesis in a solid tumor model at single-cell resolution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha D Praktiknjo et. al.PubMed 32080185
- Cancer medicine 9:1842-18542020Effects of propofol on colon cancer metastasis through STAT3/HOTAIR axis by activating WIF-1 and suppressing Wnt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Fei Zhang et. al.PubMed 31953926
- eLife 8:2019Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNona Farbehi et. al.PubMed 30912746
- Molecular medicine reports 17:8180-81882018c‑Jun suppresses the expression of WNT inhibitory factor 1 through transcriptional regulation and interaction with DNA methyltransferase 1 in gallbladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Lin et. al.PubMed 29693707
- Molecular medicine reports 17:7265-72732018Genistein inhibits invasion and migration of colon cancer cells by recovering WIF1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhu et. al.PubMed 29568950
- PeerJ 6:e44452018Expression pattern of Wif1 and -catenin during development of anorectum in fetal rats with anorectal malformations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Bing Tang et. al.PubMed 29507836
- Respiratory research 18:1522017Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function.
- Nature communications 8:158702017Simultaneous overactivation of Wnt/β-catenin and TGFβ signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunchao Cai et. al.PubMed 28627514
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WIF1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5W5
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Expected |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
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Target data
Function
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Alternative names
UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1
Recommended products
Rabbit Polyclonal WIF1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WIF1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WIF1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5W5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.
- Biological function summary
WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.
- Pathways
WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.
- Associated diseases and disorders
WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-WIF1 antibody (ab186845)
All lanes: Western blot - Anti-WIF1 antibody (ab186845) at 1/500 dilution
Lane 1: Extract of mouse trachea cell line
Lane 2: Extract of mouse heart cell line
Lane 3: Extract of mouse skeletal muscle cell line
Predicted band size: 41 kDa
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