AB186845

Anti-WIF1 antibody

Name: Anti-WIF1 antibody (ab186845) | Abcam
Brand: Abcam Limited
SKU: AB186845
Price: 535 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal WIF1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WIF1.

View Alternative Names

UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1

1 Images

Supplier Data

Western blot - Anti-WIF1 antibody (AB186845)

All lanes:

Western blot - Anti-WIF1 antibody (ab186845) at 1/500 dilution

Lane 1:

Extract of mouse trachea cell line

Lane 2:

Extract of mouse heart cell line

Lane 3:

Extract of mouse skeletal muscle cell line

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human WIF1. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5W5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.

Biological function summary

WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.

Pathways

WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.

WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

See full target information WIF1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

BioFactors (Oxford, England) 48:683-698 PubMed35080047

2022

Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen-induced retinopathy mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Tan,Huizhuo Xu,Bolin Chen,Tianqi Duan,Kangcheng Liu,Jing Zou

Nature communications 11:991 PubMed32080185

2020

Tracing tumorigenesis in a solid tumor model at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha D Praktiknjo,Benedikt Obermayer,Qionghua Zhu,Liang Fang,Haiyue Liu,Hazel Quinn,Marlon Stoeckius,Christine Kocks,Walter Birchmeier,Nikolaus Rajewsky

Cancer medicine 9:1842-1854 PubMed31953926

2020

Effects of propofol on colon cancer metastasis through STAT3/HOTAIR axis by activating WIF-1 and suppressing Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Fei Zhang,Chang-Sheng Li,Yi Zhou,Xi-Hua Lu

eLife 8: PubMed30912746

2019

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nona Farbehi,Ralph Patrick,Aude Dorison,Munira Xaymardan,Vaibhao Janbandhu,Katharina Wystub-Lis,Joshua Wk Ho,Robert E Nordon,Richard P Harvey

Molecular medicine reports 17:8180-8188 PubMed29693707

2018

c‑Jun suppresses the expression of WNT inhibitory factor 1 through transcriptional regulation and interaction with DNA methyltransferase 1 in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Lin,Haijie Hong,Xiaojie Jiang,Chengzong Li,Siyuan Zhu,Nanhong Tang,Xiaoqian Wang,Feifei She,Yanling Chen

Molecular medicine reports 17:7265-7273 PubMed29568950

2018

Genistein inhibits invasion and migration of colon cancer cells by recovering WIF1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhu,Jun Ren,Liming Tang

PeerJ 6:e4445 PubMed29507836

2018

Expression pattern of Wif1 and -catenin during development of anorectum in fetal rats with anorectal malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Bing Tang,Huan Li,Jin Zhang,Wei Lin Wang,Zheng Wei Yuan,Yu Zuo Bai

Respiratory research 18:152 PubMed28793908

2017

Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function.

Applications

IHC-P

Species

Mouse

Leema George,Ankita Mitra,Tania A Thimraj,Martin Irmler,Sangeetha Vishweswaraiah,Lars Lunding,Dorothea Hühn,Alicia Madurga,Johannes Beckers,Heinz Fehrenbach,Swapna Upadhyay,Holger Schulz,George D Leikauf,Koustav Ganguly

Nature communications 8:15870 PubMed28627514

2017

Simultaneous overactivation of Wnt/β-catenin and TGFβ signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Junchao Cai,Lishan Fang,Yongbo Huang,Rong Li,Xiaonan Xu,Zhihuang Hu,Le Zhang,Yi Yang,Xun Zhu,Heng Zhang,Jueheng Wu,Yan Huang,Jun Li,Musheng Zeng,Erwei Song,Yukai He,Li Zhang,Mengfeng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WIF1 antibody

Western blot - Anti-WIF1 antibody (AB186845)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise