AB155101

Anti-WIF1 antibody [EPR9385]

Name: Anti-WIF1 antibody [EPR9385] (ab155101) | Abcam
Brand: Abcam Limited
SKU: AB155101
Price: 365 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(24 Publications)

Rabbit Recombinant Monoclonal WIF1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 24 publications.

View Alternative Names

UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1

6 Images

Unknown

Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (AB155101)

Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.

Lane (-) : Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (ab155101)

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution

Lane 1:

HT29 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal lung tissue lysate at 10 µg

Predicted band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (ab155101) at 1/2000 dilution

All lanes:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (ab155101) at 1/5000 dilution

All lanes:

Human fetal lung tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

AbReview40527****

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution

Lanes 1 - 2:

pmHep3B whole cell lysate at 30 µg

Lanes 3 - 4:

Hep3B whole cell lysate at 30 µg

Secondary

All lanes:

Alkaline Phosphatase conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Carrier free

Anti-WIF1 antibody [EPR9385] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9385

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "For unpurified use at 1/10 - 1/100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.

Biological function summary

WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.

Pathways

WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.

WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

See full target information WIF1

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 26:183 PubMed39444000

2024

WIF-1 contributes to lupus-induced neuropsychological deficits via the CRYAB/STAT4-SHH axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Tan,Yu Fan,Xinyi Xu,Tianshu Zhang,Xiangyu Cao,Chenghao Zhang,Jun Liang,Yayi Hou,Huan Dou

Nature communications 15:363 PubMed38191471

2024

Distinct mesenchymal cell states mediate prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hubert Pakula,Mohamed Omar,Ryan Carelli,Filippo Pederzoli,Giuseppe Nicolò Fanelli,Tania Pannellini,Fabio Socciarelli,Lucie Van Emmenis,Silvia Rodrigues,Caroline Fidalgo-Ribeiro,Pier Vitale Nuzzo,Nicholas J Brady,Wikum Dinalankara,Madhavi Jere,Itzel Valencia,Christopher Saladino,Jason Stone,Caitlin Unkenholz,Richard Garner,Mohammad K Alexanderani,Francesca Khani,Francisca Nunes de Almeida,Cory Abate-Shen,Matthew B Greenblatt,David S Rickman,Christopher E Barbieri,Brian D Robinson,Luigi Marchionni,Massimo Loda

Iranian journal of basic medical sciences 26:1460-1467 PubMed37970446

2023

MiR181-5p promotes pathogenic angiogenesis of hepatopulmonary syndrome by negatively regulating Wnt inhibitor Wif1.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Guihua Li,Caiyi Li,Congwen Yang,Kaizhi Lu

Open medicine (Warsaw, Poland) 18:20230788 PubMed37786775

2023

LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tieqiu Wu,Nannan Li,Xinghui Wu,Yongchao Du,Zhiwang Tang

Journal of ginseng research 47:515-523 PubMed37397420

2023

20(S)- Protopanaxadiol suppresses hepatic stellate cell activation via WIF1 demethylation-mediated inactivation of the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxue Li,Yating Zhan,Rongrong Zhang,Qiqi Tao,Zhichao Lang,Jianjian Zheng

Critical reviews in eukaryotic gene expression 33:1-12 PubMed37183942

2023

MicroRNA-485-3p Promotes the Inflammatory Response and Extracellular Matrix Deposition by Activating Wnt/β-Catenin Signaling in Human Airway Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiyun Liu,Sen Shi,Ying Gao,Qian Leng,Rui Gong,Lan Zhang,Jinhai Ma

Heliyon 9:e16004 PubMed37206019

2023

Epigenetic regulation of human WIF1 and DNA methylation situation of WIF1 and GSTM5 in urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Huang Shen,Pei-Yu Li,Shou-Chieh Wang,Sin-Rong Wu,Chih-Yu Hsieh,Yuan-Chang Dai,Yi-Wen Liu

Journal of molecular histology 52:1067-1080 PubMed34398360

2021

Globular adiponectin inhibits osteoblastic differentiation of vascular smooth muscle cells through the PI3K/AKT and Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhou,Li-Long Wei,Rui-Ping Zhang,Cheng-Wu Han,Yongtong Cao

Aging 13:17190-17201 PubMed34229300

2021

miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongkun Wei,Huiling Ma,Haiqing Zhou,Hanrong Yin,Jie Yang,Yongcai Song,Binhui Yang

OncoTargets and therapy 14:3635-3648 PubMed34113129

2021

miR-340 Promotes Retinoblastoma Cell Proliferation, Migration and Invasion Through Targeting WIF1.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Fengmei Han,Yanping Wu,Xue Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WIF1 antibody [EPR9385]

Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (AB155101)

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

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