AB249191

Anti-WIF1 antibody [EPR9385] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal WIF1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1

5 Images

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Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

This data was developed using ab155101, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (<a href='/en-us/products/primary-antibodies/wif1-antibody-epr9385-ab155101'>ab155101</a>) at 1/2000 dilution

All lanes:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

This data was developed using ab155101, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (<a href='/en-us/products/primary-antibodies/wif1-antibody-epr9385-ab155101'>ab155101</a>) at 1/5000 dilution

All lanes:

Human fetal lung tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Immunoprecipitation - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

This data was developed using ab155101, the same antibody clone in a different buffer formulation.

Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.

Lane (-) : Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (<a href='/en-us/products/primary-antibodies/wif1-antibody-epr9385-ab155101'>ab155101</a>)

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

This data was developed using ab155101, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (<a href='/en-us/products/primary-antibodies/wif1-antibody-epr9385-ab155101'>ab155101</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

This data was developed using ab155101, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WIF1 antibody [EPR9385] (<a href='/en-us/products/primary-antibodies/wif1-antibody-epr9385-ab155101'>ab155101</a>) at 1/1000 dilution

Lane 1:

HT29 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal lung tissue lysate at 10 µg

Predicted band size: 41 kDa

false

Unconjugated

Anti-WIF1 antibody [EPR9385]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9385

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249191 is the carrier-free version of ab155101.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.

Biological function summary

WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.

Pathways

WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.

WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

See full target information WIF1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WIF1 antibody [EPR9385] - BSA and Azide free

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

Immunoprecipitation - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (AB249191)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise