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AB89935

Anti-WIF1 antibody [MM0602-6D27]

3

(1 Review)

|

(1 Publication)

Mouse Monoclonal WIF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WIF1.

View Alternative Names

UNQ191/PRO217, WIF1, Wnt inhibitory factor 1, WIF-1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0602-6D27

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human WIF1.

Q9Y5W5

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Alternative Products

Primary Antibodies

AB186845

Anti-WIF1 antibody

Western blot - Anti-WIF1 antibody (AB186845)

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Primary Antibodies

AB155101

Anti-WIF1 antibody [EPR9385]

RabMAb|Recombinant

Western blot - Anti-WIF1 antibody [EPR9385] (AB155101)

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Complementary Products

Assay Kits

AB267596

Human WIF-1 ELISA Kit

Sandwich ELISA - Human WIF-1 ELISA Kit (AB267596)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB85799

Anti-Sclerostin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Sclerostin antibody (AB85799)

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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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  • 92
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.
Biological function summary

WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.

Pathways

WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.

WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
See full target information WIF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 16:7497-7503 PubMed28944908

2017

Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinchao Deng,Congzhe Hou,Huali Wang,Tingting Liang,Lin Zhu
View all publications
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Product promise

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