AB201948

Anti-Wilms Tumor Protein antibody [5G11A5]

Name: Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) | Abcam
Brand: Abcam Limited
SKU: AB201948
Price: 500 USD
Availability: InStock
Rating: 1 (2 reviews)

(2 Reviews)

(4 Publications)

Mouse Monoclonal Wilms Tumor Protein antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WT1 aa 300 to C-terminus.

View Alternative Names

Wilms tumor protein, WT33, WT1

3 Images

Flow Cytometry - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

Flow cytometric analysis of HeLa cells labeling Wilms Tumor Protein with ab201948 at 1/200 dilution (green) compared to a negative control (red).

Supplier Data

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

Expected MWt is 47.6 kDa.

All lanes:

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) at 1/500 dilution

All lanes:

Human Wilms Tumor Protein (aa314-479) recombinant protein

Predicted band size: 22 kDa,49 kDa

false

Supplier Data

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

All lanes:

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Wilms Tumor Protein (aa314-479)-hIgGFc transfected HEK293 (2) cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G11A5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human WT1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P19544

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR278304-7 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.

Biological function summary

The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.

Pathways

WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.

WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays an important role in cellular development and cell survival (PubMed : 7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed : 17716689, PubMed : 25258363, PubMed : 7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific : isoforms lacking the KTS motif may act as transcription factors (PubMed : 15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed : 16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed : 19123921).

See full target information WT1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 14:1969-1979 PubMed37220935

2023

Circular non-coding RNA circ_0072088 serves as a ceRNA, targeting the miR-1225-5p/WT1 axis to regulate non-small cell lung cancer cell malignant behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Zhu,Jing Wan,Xu You,Wanli Yang,Lei Zhao

Oncology reports 42:2016-2028 PubMed31436300

2019

WT1 regulates cyclin A1 expression in K562 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sony Pandey,Mustafa Moazam,Nirmala Ghimirey,Steven J Kuerbitz,Gail C Fraizer

ALTEX 36:277-288 PubMed30570667

2018

Human platelet lysate as validated replacement for animal serum to assess chemosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Pons,Georg Nagel,Yanira Zeyn,Mandy Beyer,Teresa Laguna,Tina Brachetti,Andreas Sellmer,Siavosh Mahboobi,Roland Conradi,Falk Butter,Oliver H Krämer

Journal of cellular and molecular medicine 22:1650-1665 PubMed29349903

2018

GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-β1/Smad2 signalling pathway.

Applications

Species

Human

Han-Jie Yang,Ge-Liang Liu,Bo Liu,Tian Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Wilms Tumor Protein antibody [5G11A5]

Flow Cytometry - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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