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Mouse Monoclonal Wilms Tumor Protein antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WT1 aa 300 to C-terminus.


Images

Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948), expandable thumbnail
  • Flow Cytometry - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948), expandable thumbnail
  • Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (AB201948), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human WT1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P19544

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Alternative names

Wilms tumor protein, WT33, WT1

Recommended products

Mouse Monoclonal Wilms Tumor Protein antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WT1 aa 300 to C-terminus.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human WT1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P19544
Clone number
5G11A5
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to supernatant. Lot no's high than GR278304-7 are from Tissue Culture Supernatant

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.

Biological function summary

The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.

Pathways

WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.

Associated diseases and disorders

WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.

Product promise

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3 product images

  • Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948), expandable thumbnail

    Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948)

    Expected MWt is 47.6 kDa.

    All lanes: Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) at 1/500 dilution

    All lanes: Human Wilms Tumor Protein (aa314-479) recombinant protein

    Predicted band size: 22 kDa, 49 kDa

  • Flow Cytometry - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948), expandable thumbnail

    Flow Cytometry - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948)

    Flow cytometric analysis of HeLa cells labeling Wilms Tumor Protein with ab201948 at 1/200 dilution (green) compared to a negative control (red).

  • Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948), expandable thumbnail

    Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948)

    All lanes: Western blot - Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) at 1/500 dilution

    Lane 1: HEK293 cell lysate

    Lane 2: Wilms Tumor Protein (aa314-479)-hIgGFc transfected HEK293 (2) cell lysate

    Predicted band size: 49 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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