Rabbit Polyclonal Wilms Tumor Protein antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WT1.
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- Frontiers in oncology 11:7442562021Patient-Derived Xenografts Are a Reliable Preclinical Model for the Personalized Treatment of Epithelial Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayu Chen,Ying Jin,Siyi Li,Cui Qiao,Xinxin Peng,Yan Li,Yu Gu,Wei Wang,Yan You,Jie Yin,Ying Shan,Yong-Xue Wang,Meng Qin,Hongyue Li,Yan Cai,Yu Dong,Siying Peng,Lingya PanPubMed 34671560
- Stem cells international 2021:88590712021Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Liu,Dinghui Wang,Tianhua Xiong,Yajie Liu,Xiaodong Jing,Jianlin Du,Qiang ShePubMed 33897781
- Frontiers in cell and developmental biology 9:6167112021The miR-203a Regulatory Network Affects the Proliferation of Chronic Myeloid Leukemia K562 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua He,Zeping Han,Ziyi An,Yumin Li,Xingyi Xie,Jiabin Zhou,Sihua He,Yubing Lv,Mengling He,Hong Qu,Gexiu Liu,Yuguang LiPubMed 33659248
- American journal of physiology. Renal physiology 320:F548-F5582021Lipotoxicity dysregulates the immunoproteasome in podocytes and kidneys in type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Soon Lee,Ji Yeon Suh,Byeong-Choel Kang,Eugene LeePubMed 33586497
- BioMed research international 2020:70690522020Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3K/AKT/mTOR and Nrf2/HO-1 Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiu Di Tu et. al.PubMed 33204708
- Experimental and therapeutic medicine 19:123-1302019Resveratrol alleviates obesity-associated podocyte injury in ovariectomized obese rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Li,Xiaoyan Xiao,Yulian Miao,Ling Guo,Junhui Zhen,Xianhua Li,Bei Jiang,Zhao HuPubMed 31853281
- Endocrinology 161:2019Primary Sertoli Cell Cultures From Adult Mice Have Different Properties Compared With Those Derived From 20-Day-Old Animals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpornrad Saewu,Kessiri Kongmanas,Riya Raghupathy,Jacob Netherton,Suraj Kadunganattil,James-Jules Linton,Watchadaporn Chaisuriyong,Kym F Faull,Mark A Baker,Nongnuj TanphaichitrPubMed 31730175
- Biochemical pharmacology 166:139-1522019Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTu-Shuai Li et. al.PubMed 31085161
- Biology of reproduction 97:497-5132017Deletion of Spata2 by CRISPR/Cas9n causes increased inhibin alpha expression and attenuated fertility in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhao,Jianjun Zhao,Guojin Xu,Zhijuan Wang,Jie Gao,Sheng Cui,Jiali LiuPubMed 29025062
- Molecular medicine reports 16:6142-61472017Epigallocatechin‑3‑gallate protects from high glucose induced podocyte apoptosis via suppressing endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunhong Xiang et. al.PubMed 28901409
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human WT1. Database link P19544
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Alternative names
Wilms tumor protein, WT33, WT1
Recommended products
Rabbit Polyclonal Wilms Tumor Protein antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WT1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human WT1. Database link P19544
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.
- Biological function summary
The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.
- Pathways
WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.
- Associated diseases and disorders
WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Wilms Tumor Protein antibody (ab180840)
Blocking buffer: 3% nonfat dry milk in TBST.
All lanes: Western blot - Anti-Wilms Tumor Protein antibody (ab180840) at 1/500 dilution
Lane 1: A549 cell lysate at 25 µg
Lane 2: MCF7 cell lysate at 25 µg
Lane 3: Mouse heart tissue lysate at 25 µg
Lane 4: Mouse testis tissue lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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