Rabbit Recombinant Monoclonal Wilms Tumor Protein antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103805.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425)")
![Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--flow-cytometry-intracellular-img37549.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16089.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57407.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425)")
![Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--flow-cytometry-intracellular-img51939.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (AB242425)")
Publications
Filter by
- Matrix biology : journal of the International Society for Matrix Biology 98:32-482021Loss of sphingosine kinase 2 enhances Wilm's tumor suppressor gene 1 and nephrin expression in podocytes and protects from streptozotocin-induced podocytopathy and albuminuria in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFaik Imeri,Bisera Stepanovska Tanturovska,Stephanie Schwalm,Sarbari Saha,Jinyang Zeng-Brouwers,Herrmann Pavenstädt,Josef Pfeilschifter,Liliana Schaefer,Andrea HuwilerPubMed 34015468
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Primary incubation for 30 minutes at 4°C. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Primary incubation for 1 hour at room temperature. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
Species Rat | Dilution info - | Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
Species Mouse | Dilution info - | Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
Target data
Function
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Alternative names
Wilms tumor protein, WT33, WT1
Recommended products
Rabbit Recombinant Monoclonal Wilms Tumor Protein antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP321
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab242425 is the carrier-free version of Anti-Wilms Tumor Protein antibody [SP321] ab224806.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.
- Biological function summary
The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.
- Pathways
WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.
- Associated diseases and disorders
WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103805.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Wilms Tumor Protein with Anti-Wilms Tumor Protein antibody [SP321] ab224806 at 1/100 dilution (2.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Wilms Tumor Protein antibody [SP321] ab224806)![Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--flow-cytometry-intracellular-img37549.jpg "Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)
Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling Wilms Tumor Protein with purified Anti-Wilms Tumor Protein antibody [SP321] ab224806 at 1:20 dilution (12.6 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242425)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16089.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Wilms Tumor Protein with Anti-Wilms Tumor Protein antibody [SP321] ab224806 at 1/100 dilution (2.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Wilms Tumor Protein antibody [SP321] ab224806)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57407.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Wilms Tumor Protein with Anti-Wilms Tumor Protein antibody [SP321] ab224806 at 1/100 dilution (2.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Wilms Tumor Protein antibody [SP321] ab224806)![Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--flow-cytometry-intracellular-img51939.jpg "Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)
Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Wilms Tumor Protein with Anti-Wilms Tumor Protein antibody [SP321] ab224806 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Wilms Tumor Protein antibody [SP321] ab224806).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112886.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Wilms Tumor Protein using Anti-Wilms Tumor Protein antibody [SP321] ab224806 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Wilms Tumor Protein antibody [SP321] ab224806).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425), expandable thumbnail](https://content.abcam.com/products/images/wilms-tumor-protein-antibody-sp321-bsa-and-azide-free-ab242425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112886.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] - BSA and Azide free (ab242425)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com