AB220212

Anti-Wilms Tumor Protein antibody [WT1/857]

Name: Anti-Wilms Tumor Protein antibody [WT1/857] (ab220212) | Abcam
Brand: Abcam Limited
SKU: AB220212
Price: 520 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal Wilms Tumor Protein antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WT1.

View Alternative Names

Wilms tumor protein, WT33, WT1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human fetal kidney tissue labeling Wilms Tumor protein with ab220212 at 1 μg/mL.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human Mesothelioma tissue labeling Wilms Tumor protein with ab220212 at 1 μg/mL.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human testis tissue labeling Wilms Tumor protein with ab220212 at 1 μg/mL.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat testis tissue labeling Wilms Tumor protein with ab220212 at 1 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WT1/857

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human WT1. The exact immunogen used to generate this antibody is proprietary information.

P19544

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.

Biological function summary

The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.

Pathways

WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.

WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays an important role in cellular development and cell survival (PubMed : 7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed : 17716689, PubMed : 25258363, PubMed : 7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific : isoforms lacking the KTS motif may act as transcription factors (PubMed : 15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed : 16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed : 19123921).

See full target information WT1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Virchows Archiv : an international journal of pathology 486:1049-1059 PubMed39271482

2024

Aberrant localization of β1 integrin in podocyte cytoplasm of primary FSGS with cellular lesion.

Applications

Unspecified application

Species

Unspecified reactive species

Eisuke Katafuchi,Satoshi Hisano,Satoko Kurata,Kumiko Muta,Noriko Uesugi,Tetsu Miyamoto,Yoshikazu Harada,Shohei Shimajiri,Ritsuko Katafuchi,Toshiyuki Nakayama

Cell proliferation 54:e13055 PubMed33987885

2021

Activated mesangial cells induce glomerular endothelial cells proliferation in rat anti-Thy-1 nephritis through VEGFA/VEGFR2 and Angpt2/Tie2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghua Zhao,Bo Fu,Pu Chen,Qinggang Li,Qing Ouyang,Chuyue Zhang,Guangyan Cai,Lingling Wu,Xiangmei Chen

Journal of cellular and molecular medicine 25:1012-1023 PubMed33332718

2020

Salvianolate ameliorates oxidative stress and podocyte injury through modulation of NOX4 activity in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yiran Liang,Hong Liu,Yi Fang,Pan Lin,Zhihui Lu,Pan Zhang,Xiaoyan Jiao,Jie Teng,Xiaoqiang Ding,Yan Dai

Cellular physiology and biochemistry : internation 44:2138-2146 PubMed29241190

2017

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangmin Zhao,Le Li,Qian Wang,Hongxiu Han,Qing Zhan,Ming Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Wilms Tumor Protein antibody [WT1/857]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [WT1/857] (AB220212)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise