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Rabbit Polyclonal WIPI1 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human WIPI1.

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Images

Western blot - Anti-WIPI1 antibody (AB139722), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human WIPI1. The exact immunogen used to generate this antibody is proprietary information. Database link Q5MNZ9

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Rat
Tested

Tested
Tested

Species
Rat
Dilution info
1.00000-2.00000 µg/mL
Notes

-

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Target data

Function

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:15602573, PubMed:20114074, PubMed:20484055, PubMed:20639694, PubMed:23088497, PubMed:28561066, PubMed:31271352). Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (PubMed:28561066). Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated (PubMed:28561066). Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes (PubMed:28561066, PubMed:31271352, PubMed:33499712). There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed:28561066). Together with WDR45/WIPI4, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352). Involved in xenophagy of Staphylococcus aureus (PubMed:22829830). Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation (PubMed:22829830). Also plays a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy (PubMed:21317285). May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (PubMed:15020712).

Alternative names

WIPI49, WIPI1, WD repeat domain phosphoinositide-interacting protein 1, WIPI-1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa

Recommended products

Rabbit Polyclonal WIPI1 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human WIPI1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human WIPI1. The exact immunogen used to generate this antibody is proprietary information. Database link Q5MNZ9
Purification technique
Affinity purification Immunogen
Specificity

At least two isoforms of WIPI1 are known to exist; ab139722 will detect both isoforms.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle, Store undiluted

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

WIPI1 also known as WD repeat domain phosphoinositide-interacting protein 1 is a protein involved in cellular processes especially autophagy. WIPI1 has a molecular mass of approximately 50 kDa and consists of WD40 repeats a common motif in proteins involved in signal transduction and cell cycle control. This protein is found in various human tissues with high expression in the heart skeletal muscle and pancreas indicating its involvement in essential metabolic and regulatory activities.

Biological function summary

WIPI1 has an important role in the process of autophagy acting as part of the PI3P-binding proteins family. It functions as a component of the autophagy initiation complex contributing to the formation of autophagosomes by recruiting additional autophagic proteins. WIPI1 interacts with ATG2 proteins facilitating the elongation of isolation membranes. Its activity is influenced by the availability of phosphatidylinositol 3-phosphate (PI3P) which is a critical lipid signaling molecule in cells.

Pathways

WIPI1 participates in the mechanistic target of rapamycin (mTOR) signaling pathway and the PI3K-Akt signaling pathway. In the mTOR pathway WIPI1 negatively regulates mTORC1 signaling therefore promoting autophagosome formation under nutrient-deprived conditions. This interaction positions WIPI1 as an inhibitor of mTORC1-mediated nutrient sensing. In collaboration with ULK1 and BECN1 WIPI1 supports autophagic flux by assisting in the early stages of autophagosome formation therefore contributing to cellular homeostasis.

Associated diseases and disorders

Abnormalities in WIPI1 expression and function have links to cancer and neurodegenerative diseases. In certain types of cancer including breast cancer altered levels of WIPI1 correlate with tumor progression and poor prognosis potentially through interactions with mTOR. Additionally in neurodegenerative conditions like Alzheimer's disease dysregulated autophagy pathways involving WIPI1 and ATG proteins may lead to accumulation of damaged proteins and organelles accentuating disease symptoms.

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1 product image

  • Western blot - Anti-WIPI1 antibody (ab139722), expandable thumbnail

    Western blot - Anti-WIPI1 antibody (ab139722)

    Lane 1: Western blot - Anti-WIPI1 antibody (ab139722) at 1 µg/mL

    Lane 2: Western blot - Anti-WIPI1 antibody (ab139722) at 2 µg/mL

    Lane 1: Rat colon tissue lysate at 15 µg

    Lane 2: Rat colon tissue lysate at 15 µg

    Predicted band size: 49 kDa

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Product protocols

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