Rabbit Recombinant Monoclonal WIPI1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (AB248208), expandable thumbnail](https://content.abcam.com/products/images/wipi1-antibody-epr8110-bsa-and-azide-free-ab248208--western-blot-img102616.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (AB248208)")
![OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (AB248208), expandable thumbnail](https://content.abcam.com/products/images/wipi1-antibody-epr8110-bsa-and-azide-free-ab248208--oi-rd-scanning-img53542.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (AB248208)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Expected | Not recommended | Not recommended | Not recommended |
| Rat | Expected | Expected | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:15602573, PubMed:20114074, PubMed:20484055, PubMed:20639694, PubMed:23088497, PubMed:28561066, PubMed:31271352). Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy (PubMed:28561066). Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated (PubMed:28561066). Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes (PubMed:28561066, PubMed:31271352, PubMed:33499712). There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed:28561066). Together with WDR45/WIPI4, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352). Involved in xenophagy of Staphylococcus aureus (PubMed:22829830). Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation (PubMed:22829830). Also plays a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy (PubMed:21317285). May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway (PubMed:15020712).
Alternative names
WIPI49, WIPI1, WD repeat domain phosphoinositide-interacting protein 1, WIPI-1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa
Recommended products
Rabbit Recombinant Monoclonal WIPI1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8110
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.14 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248208 is the carrier-free version of Anti-WIPI1 antibody [EPR8110] ab128901.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WIPI1 also known as WD repeat domain phosphoinositide-interacting protein 1 is a protein involved in cellular processes especially autophagy. WIPI1 has a molecular mass of approximately 50 kDa and consists of WD40 repeats a common motif in proteins involved in signal transduction and cell cycle control. This protein is found in various human tissues with high expression in the heart skeletal muscle and pancreas indicating its involvement in essential metabolic and regulatory activities.
- Biological function summary
WIPI1 has an important role in the process of autophagy acting as part of the PI3P-binding proteins family. It functions as a component of the autophagy initiation complex contributing to the formation of autophagosomes by recruiting additional autophagic proteins. WIPI1 interacts with ATG2 proteins facilitating the elongation of isolation membranes. Its activity is influenced by the availability of phosphatidylinositol 3-phosphate (PI3P) which is a critical lipid signaling molecule in cells.
- Pathways
WIPI1 participates in the mechanistic target of rapamycin (mTOR) signaling pathway and the PI3K-Akt signaling pathway. In the mTOR pathway WIPI1 negatively regulates mTORC1 signaling therefore promoting autophagosome formation under nutrient-deprived conditions. This interaction positions WIPI1 as an inhibitor of mTORC1-mediated nutrient sensing. In collaboration with ULK1 and BECN1 WIPI1 supports autophagic flux by assisting in the early stages of autophagosome formation therefore contributing to cellular homeostasis.
- Associated diseases and disorders
Abnormalities in WIPI1 expression and function have links to cancer and neurodegenerative diseases. In certain types of cancer including breast cancer altered levels of WIPI1 correlate with tumor progression and poor prognosis potentially through interactions with mTOR. Additionally in neurodegenerative conditions like Alzheimer's disease dysregulated autophagy pathways involving WIPI1 and ATG proteins may lead to accumulation of damaged proteins and organelles accentuating disease symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208), expandable thumbnail](https://content.abcam.com/products/images/wipi1-antibody-epr8110-bsa-and-azide-free-ab248208--western-blot-img102616.jpg "Western blot - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208)")
Western blot - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208)
This data was developed using Anti-WIPI1 antibody [EPR8110] ab128901, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-WIPI1 antibody [EPR8110] (Anti-WIPI1 antibody [EPR8110] ab128901) at 1/1000 dilution
Lane 1: A375 cell lysate at 10 µg
Lane 2: BxPC-3 cell lysate at 10 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
Lane 5: RAW 264.7 cell lysate at 10 µg
Lane 6: NIH/3T3 cell lysate at 10 µg
Lane 7: JAR cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208), expandable thumbnail](https://content.abcam.com/products/images/wipi1-antibody-epr8110-bsa-and-azide-free-ab248208--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137345.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208)
This data was developed using Anti-WIPI1 antibody [EPR8110] ab128901, the same antibody clone in a different buffer formulation.Anti-WIPI1 antibody [EPR8110] ab128901 at 1/50 dilution staining WIPI1 in Paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208), expandable thumbnail](https://content.abcam.com/products/images/wipi1-antibody-epr8110-bsa-and-azide-free-ab248208--oi-rd-scanning-img53542.jpg "OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208)")
OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] - BSA and Azide free (ab248208)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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