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AB229225

Anti-WIPI2 antibody - C-terminal

5

(2 Reviews)

|

(5 Publications)

Rabbit Polyclonal WIPI2 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human WIPI2.

View Alternative Names

CGI-50, WIPI2, WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2

4 Images
Western blot - Anti-WIPI2 antibody - C-terminal (AB229225)
  • WB

Supplier Data

Western blot - Anti-WIPI2 antibody - C-terminal (AB229225)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-WIPI2 antibody - C-terminal (ab229225) at 1/2000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract at 30 µg

Lane 2:

THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 49 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI2 antibody - C-terminal (AB229225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI2 antibody - C-terminal (AB229225)

Paraffin-embedded rat liver tissue stained for WIPI2 using ab229225 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-WIPI2 antibody - C-terminal (AB229225)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WIPI2 antibody - C-terminal (AB229225)

Ice-cold methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for WIPI2 (green) using ab229225 at 1/2500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Western blot - Anti-WIPI2 antibody - C-terminal (AB229225)
  • WB

Supplier Data

Western blot - Anti-WIPI2 antibody - C-terminal (AB229225)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-WIPI2 antibody - C-terminal (ab229225) at 1/2000 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human WIPI2. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4P8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIPI2 also known as WD repeat domain phosphoinositide-interacting protein 2 functions as a critical component in autophagy a cellular degradation process. This protein weighs approximately 46 kDa and plays an important role in the initiation of autophagosome formation by binding to phosphatidylinositol 3-phosphate on lipid bilayers. Its expression is detected in various tissues with significant abundance in skeletal muscle heart and brain. These expression patterns suggest a broad involvement in cellular regulation and maintenance.
Biological function summary

WIPI2 serves as an important scaffold in the formation of the autophagy machinery. It is part of a larger complex that includes key proteins like Atg16L1 and ULK1 which are essential in the autophagy process. WIPI2 assists in recruiting additional autophagic factors to the developing autophagosome contributing to the membrane curvature and expansion necessary for cargo sequestration. Its activity ensures the efficient turnover of damaged organelles and proteins maintaining cellular health and homeostasis.

Pathways

WIPI2 integrates into the autophagy and PI3K/Akt signaling pathways. Each of these pathways plays a significant role in cellular response to nutrient deprivation and stress. In the autophagy pathway WIPI2 directly interacts with proteins like LC3 facilitating the biogenesis of autophagosomes. Through the PI3K/Akt pathway WIPI2 supports cell survival and growth regulation linking metabolic status with autophagic responses. This interaction highlights its importance in cellular adaptation to environmental changes.

WIPI2 shows a strong connection to neurodegenerative diseases and cancer. Dysfunction in WIPI2's activity has been implicated in the progression of neurodegenerative disorders like Parkinson's Disease where impaired autophagy contributes to the accumulation of misfolded proteins. In cancer altered WIPI2 expression and function are observed linking it with tumorigenesis and cancer cell survival potentially through interactions with proteins such as mTOR and AMPK. Understanding WIPI2’s role in these conditions could lead to novel therapeutic strategies that target autophagy modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed : 20505359, PubMed : 28561066). Involved in an early step of the formation of preautophagosomal structures (PubMed : 20505359, PubMed : 28561066). Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases (PubMed : 28561066). Mediates ER-isolation membranes contacts by interacting with the ULK1 : RB1CC1 complex and PtdIns3P (PubMed : 28890335). Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed : 20505359, PubMed : 28561066).. Isoform 4. Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.
See full target information WIPI2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:77 PubMed39934774

2025

sdRNA-D43 derived from small nucleolar RNA snoRD43 improves chondrocyte senescence and osteoarthritis progression by negatively regulating PINK1/Parkin-mediated mitophagy pathway via dual-targeting NRF1 and WIPI2.

Applications

Unspecified application

Species

Unspecified reactive species

Zengfa Deng,Changzhao Li,Shu Hu,Yanlin Zhong,Wei Li,Zhencan Lin,Xiaolin Mo,Ming Li,Dongliang Xu,Dianbo Long,Guping Mao,Yan Kang

Autophagy 20:1537-1558 PubMed38591519

2024

Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Macke,Taylor E Divita,Artem N Pachikov,Sundararajan Mahalingam,Ramesh Bellamkonda,Karuna Rasineni,Carol A Casey,Armen Petrosyan

Nature communications 14:7338 PubMed37957156

2023

Local membrane source gathering by p62 body drives autophagosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhao Feng,Daxiao Sun,Yanchang Li,Jinpei Zhang,Shiyu Liu,Dachuan Zhang,Jingxiang Zheng,Qing Xi,Haisha Liang,Wenkang Zhao,Ying Li,Mengbo Xu,Jiayu He,Tong Liu,Ayshamgul Hasim,Meisheng Ma,Ping Xu,Na Mi

Cell death & disease 14:52 PubMed36681682

2023

Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Rung Wu,Shang-Yin Chiang,Robert Midence,Wen-Chao Kao,Chun-Lun Lai,I-Cheng Cheng,Shen-Ju Chou,Chih-Cheng Chen,Chih-Yang Huang,Ruey-Hwa Chen

Evidence-based complementary and alternative medic 2020:8878259 PubMed33414841

2020

Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Caigu He,Xuehua Zheng,Xiuhong Lin,Xinying Chen,Chenyi Shen
View all publications
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