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Rabbit Recombinant Monoclonal WISP1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-WISP-1/CCN4 antibody [EPR22891-66] - BSA and Azide free (Detector) (AB280990), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.

Alternative names

WISP1, CCN4, CCN family member 4, WNT1-inducible-signaling pathway protein 1, Wnt-1-induced secreted protein, WISP-1

Recommended products

Rabbit Recombinant Monoclonal WISP1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22891-66
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab280990 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human WISP-1 (CCN4). The recommended pair for sandwich ELISA is:
Capture: Anti-WISP1 antibody [EPR22891-92] - BSA and Azide free (Capture) ab281140, Human WISP-1 (CCN4) Capture Antibody (unconjugated)
Detector: ab280990, Human WISP-1 (CCN4) Detector Antibody (unconjugated)
The reference range value is 78.13-5000 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

WISP-1 also known as CCN4 is a protein closely associated with cell signaling and extracellular matrix interactions. It is a member of the CCN family of proteins with a molecular mass of approximately 40 kDa. This protein is expressed in various tissues including bone skin and adipose tissue. WISP-1 does not act alone but interacts with integrins and other extracellular matrix proteins influencing cellular communication and tissue homeostasis.

Biological function summary

WISP-1 influences cell proliferation migration and differentiation. It plays a role in the regulation of the skeletal system and is involved in maintaining the balance of bone formation and resorption. WISP-1 can form part of protein complexes that affect the adhesion of cells and modulate cellular responses to growth factors. These interactions underline WISP-1's involvement in promoting or inhibiting cell growth depending on the cellular context.

Pathways

WISP-1 is integral to the Wnt signaling pathway and the TGF-beta pathway. These pathways participate in many developmental processes and are essential for tissue repair and regeneration. Through the Wnt pathway WISP-1 interacts with proteins like beta-catenin influencing gene expression that regulates cellular proliferation. In the TGF-beta pathway it may associate with Smad proteins contributing to cellular differentiation and immune responses.

Associated diseases and disorders

WISP-1 relates to osteoarthritis and certain cancer types. In osteoarthritis WISP-1 modulates cartilage degradation suggesting its role in the progression of joint degeneration. WISP-1's link with cancer involves interactions with proteins such as P53 where it may promote or suppress tumor growth depending on the context. These associations make WISP-1 a target of interest in drug development and therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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