Rabbit Polyclonal WISP1 antibody. C-terminal. Suitable for WB, IHC-Fr, IHC-FoFr and reacts with Rat, Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human CCN4 aa 350 to C-terminus.
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- International journal of molecular sciences 23:2022WNT1 Inducible Signaling Pathway Protein 1 Is a Stroma-Specific Secreting Protein Inducing a Fibroblast Contraction and Carcinoma Cell Growth in the Human Prostate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKang-Shuo Chang,Syue-Ting Chen,Hsin-Ching Sung,Shu-Yuan Hsu,Wei-Yin Lin,Chen-Pang Hou,Yu-Hsiang Lin,Tsui-Hsia Feng,Ke-Hung Tsui,Horng-Heng JuangPubMed 36232736
- International journal of endocrinology 2022:39191612022Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeizhong Peng,Hanying Liu,Qingxuan Ji,Pan Ma,Yiting Niu,Shangqiu Ning,Huihui Sun,Xinxin Pang,Yuqian Yang,Yuting Zhang,Jing Han,Gaimei HaoPubMed 36237833
- Fluids and barriers of the CNS 18:442021Activation of Frizzled-7 attenuates blood-brain barrier disruption through Dvl/β-catenin/WISP1 signaling pathway after intracerebral hemorrhage in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei He,Qin Lu,Prativa Sherchan,Lei Huang,Xin Hu,John H Zhang,Haibin Dai,Jiping TangPubMed 34565396
- American journal of translational research 12:7297-73112020WISP1 indicates poor prognosis and regulates cell proliferation and apoptosis in gastric cancer via targeting AKT/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyan Zhu et. al.PubMed 33312368
- Hepatology communications 4:1056-10722020Activation of Adenosine Monophosphate-Activated Protein Kinase Reduces the Onset of Diet-Induced Hepatocellular Carcinoma in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDieter Schmoll et. al.PubMed 32626837
- Inflammation 43:425-4322020WISP1 and TLR4 on Macrophages Contribute to Ventilator-Induced Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuang Yu,Tingting Wang,Liming Zhang,Xiaohu Yang,Quan Li,Xibing DingPubMed 32130574
- Respiratory research 20:2332019Down-regulation of long non-coding RNA SNHG14 protects against acute lung injury induced by lipopolysaccharide through microRNA-34c-3p-dependent inhibition of WISP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinyuan Zhu et. al.PubMed 31660971
- World journal of gastroenterology 25:5814-58252019Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Hua Zhang,Yan Wang,Qian-Qian Fan,Yan-Kui Liu,Long-Hai Li,Xiao-Wei Qi,Yong Mao,Dong HuaPubMed 31636474
- Journal of cellular physiology 235:2009-20222019WNT1-inducible signaling protein-1 mediates TGF-β1-induced renal fibrosis in tubular epithelial cells and unilateral ureteral obstruction mouse models via autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Yang,Huan Wang,Yueju Tu,Yi Li,Yurong Zou,Guisen Li,Li Wang,Xiang ZhongPubMed 31512238
- Journal of cellular biochemistry 121:1250-12592019Guanylate-binding protein-2 inhibits colorectal cancer cell growth and increases the sensitivity to paclitaxel of paclitaxel-resistant colorectal cancer cells by interfering Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Hui Min,Bin Hu,Xiaorong Xue,Yufan LiuPubMed 31489998
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CCN4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95388
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Reactivity data
Select an application| WB | IHC-Fr | IHC-FoFr | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.1-0.5 µg/mL | Notes - |
Species Mouse | Dilution info 0.1-0.5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
Alternative names
WISP1, CCN4, CCN family member 4, WNT1-inducible-signaling pathway protein 1, Wnt-1-induced secreted protein, WISP-1
Recommended products
Rabbit Polyclonal WISP1 antibody. C-terminal. Suitable for WB, IHC-Fr, IHC-FoFr and reacts with Rat, Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human CCN4 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CCN4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95388
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.
- Biological function summary
WISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.
- Pathways
The network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.
- Associated diseases and disorders
WISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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9 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling WISP1 with ab178547 at 1 μg/ml.
Western blot - Anti-WISP1 antibody - C-terminal (ab178547)
All lanes: Western blot - Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/mL
Lane 1: Rat heart tissue lysate at 50 µg
Lane 2: Rat kidney tissue lysate at 50 µg
Lane 3: Rat lung tissue lysate at 50 µg
Lane 4: A549 whole cell lysate at 40 µg
Lane 5: A431 whole cell lysate at 40 µg
Lane 6: COLO320 whole cell lysate at 40 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunocytochemistry - Anti-WISP1 antibody - C-terminal (ab178547)
Immunocytochemical analysis of MCF7 cells labeling WISP1 with ab178547 at 1 μg/ml.
Immunohistochemistry (Frozen sections) - Anti-WISP1 antibody - C-terminal (ab178547)
Immunohistochemical analysis of frozen rat cardiac muscle sections labeling WISP1 with ab178547 at 1μg/mL (incubated overnight at 4°C). Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling WISP1 with ab178547 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling WISP1 with ab178547 at 1 μg/ml.
Western blot - Anti-WISP1 antibody - C-terminal (ab178547)
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab178547 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for WISP1 at approximately 45 kDa. The expected band size for WISP1 is at 40 kDa.All lanes: Western blot - Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/mL
Lane 1: rat kidney tissue lysates at 30 µg
Lane 2: mouse kidney tissue lysates at 30 µg
SecondaryAll lanes: goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
WISP1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab178547 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
WISP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab178547 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
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