Rabbit Recombinant Monoclonal WISP1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
Images
![Sandwich ELISA - Anti-WISP1 antibody [EPR23996-175] - BSA and Azide free (Capture) (AB284970), expandable thumbnail](https://content.abcam.com/products/images/wisp1-antibody-epr23996-175-bsa-and-azide-free-capture-ab284970--sandwich-elisa-img174743.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-WISP1 antibody [EPR23996-175] - BSA and Azide free (Capture) (AB284970)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Expected |
| Rat | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity). Suppresses tumor growth in vivo.
Alternative names
Elm1, Wisp1, Ccn4, CCN family member 4, ELM-1, WNT1-inducible-signaling pathway protein 1, WISP-1
Recommended products
Rabbit Recombinant Monoclonal WISP1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23996-175
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284970 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse WISP1. The recommended pair for sandwich ELISA is:
Capture: ab284970, Mouse WISP1 Capture Antibody (unconjugated)
Detector: Anti-WISP1 antibody [EPR23996-174] - BSA and Azide free (Detector) ab285076, Mouse WISP1 Detector Antibody (unconjugated)
The reference range value is 16 - 1000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.
- Biological function summary
WISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.
- Pathways
The network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.
- Associated diseases and disorders
WISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-WISP1 antibody [EPR23996-175] - BSA and Azide free (Capture) (ab284970), expandable thumbnail](https://content.abcam.com/products/images/wisp1-antibody-epr23996-175-bsa-and-azide-free-capture-ab284970--sandwich-elisa-img174743.jpg "Sandwich ELISA - Anti-WISP1 antibody [EPR23996-175] - BSA and Azide free (Capture) (ab284970)")
Sandwich ELISA - Anti-WISP1 antibody [EPR23996-175] - BSA and Azide free (Capture) (ab284970)
Example of Mouse WISP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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