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AB224720

Anti-WISP3 antibody

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(3 Publications)

Rabbit Polyclonal WISP3 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCN6 aa 200 to C-terminus.

View Alternative Names

WISP3, UNQ462/PRO790/PRO956, CCN6, Cellular communication network factor 6, CCN family member 6, WNT1-inducible-signaling pathway protein 3, WISP-3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP3 antibody (AB224720)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP3 antibody (AB224720)

Paraffin-embedded human liver cancer tissue stained for WISP3 using ab224720 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-WISP3 antibody (AB224720)
  • WB

Supplier Data

Western blot - Anti-WISP3 antibody (AB224720)

All lanes:

Western blot - Anti-WISP3 antibody (ab224720) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 2:

Rat brain lysate

Lane 3:

Rat heart lysate

Lane 4:

Mouse heart lysate

Lane 5:

Mouse brain lysate

Lane 6:

Mouse kidney lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP3 antibody (AB224720)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP3 antibody (AB224720)

Paraffin-embedded human kidney tissue stained for WISP3 using ab224720 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CCN6 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95389

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WISP3 also known as CCN6 is a protein with a mass of approximately 39 kDa. It belongs to the CCN family of proteins which are involved in diverse cellular processes. WISP3 is primarily expressed in cartilage and connective tissues such as bone and skin. This protein interacts with growth factors and other signaling molecules influencing cellular growth and maintenance. WISP3's mechanical role involves binding to extracellular matrix components which affects cell adhesion and communication.
Biological function summary

WISP3 functions as an important modulator of cartilage homeostasis and matrix production. It takes part in processes that regulate cartilage integrity and interacts with other extracellular proteins to maintain the physical properties of connective tissues. One of its roles includes modulating interactions with the insulin-like growth factor (IGF) binding proteins. These interactions support tissue structural integrity and cellular communication essential for development and repair mechanisms.

Pathways

WISP3 participates in the regulation of the Wnt signaling pathway. This pathway plays an important role in chondrogenesis and osteogenesis processes vital for bone and cartilage development. WISP3 influences the activity of key proteins within this pathway such as Wnt and Frizzled to modulate gene expression. It also interacts with proteins like beta-catenin which are pivotal to these signaling cascades therefore contributing to tissue specification and differentiation processes.

WISP3 mutations are associated with Progressive Pseudorheumatoid Dysplasia (PPD) and have connections to conditions affecting skeletal development. PPD is a disorder characterized by cartilage degeneration leading to joint pain and stiffness. WISP3's interaction with IGF-related proteins is critical in these conditions as dysregulation can lead to improper tissue development and homeostasis. Studies also link abnormal WISP3 activity to osteoarthritis where its altered expression might contribute to the disease pathology by affecting cartilage cell signaling and matrix production.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mitochondrial electron transport and mitochondrial respiration (PubMed : 27252383). Through its regulation of the mitochondrial function may play a role in normal postnatal skeletal growth and cartilage homeostasis (PubMed : 10471507, PubMed : 27252383).
See full target information CCN6
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 16:433-445 PubMed34811632

2021

The matricellular protein CCN6 differentially regulates mitochondrial metabolism in normal epithelium and in metaplastic breast carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Tran,Shoshana A Leflein,Maria E Gonzalez,Celina G Kleer

Breast cancer research and treatment 172:577-586 PubMed30220054

2018

CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast.

Applications

Unspecified application

Species

Unspecified reactive species

Emily R McMullen,Maria E Gonzalez,Stephanie L Skala,Mai Tran,Dafydd Thomas,Sabra I Djomehri,Boris Burman,Kelley M Kidwell,Celina G Kleer

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 99:306-312 PubMed29353205

2018

WISP3 prevents fibroblast-myofibroblast transdifferentiation in NRK-49F cells.

Applications

IHC-P

Species

Unspecified reactive species

Yang Yi,Jun Ma,Lu Jianrao,Hangqing Wang,Yingdan Zhao
View all publications
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Product promise

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