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AB187666

Anti-WISP3 antibody [EPR14769] - C-terminal

3

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal WISP3 antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

WISP3, UNQ462/PRO790/PRO956, CCN6, Cellular communication network factor 6, CCN family member 6, WNT1-inducible-signaling pathway protein 3, WISP-3

2 Images
Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (AB187666)
  • WB

Supplier Data

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (AB187666)

All lanes:

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666) at 1/1000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (AB187666)
  • WB

Supplier Data

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (AB187666)

All lanes:

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

  • Carrier free

    Anti-WISP3 antibody [EPR14769] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14769

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WISP3 also known as CCN6 is a protein with a mass of approximately 39 kDa. It belongs to the CCN family of proteins which are involved in diverse cellular processes. WISP3 is primarily expressed in cartilage and connective tissues such as bone and skin. This protein interacts with growth factors and other signaling molecules influencing cellular growth and maintenance. WISP3's mechanical role involves binding to extracellular matrix components which affects cell adhesion and communication.
Biological function summary

WISP3 functions as an important modulator of cartilage homeostasis and matrix production. It takes part in processes that regulate cartilage integrity and interacts with other extracellular proteins to maintain the physical properties of connective tissues. One of its roles includes modulating interactions with the insulin-like growth factor (IGF) binding proteins. These interactions support tissue structural integrity and cellular communication essential for development and repair mechanisms.

Pathways

WISP3 participates in the regulation of the Wnt signaling pathway. This pathway plays an important role in chondrogenesis and osteogenesis processes vital for bone and cartilage development. WISP3 influences the activity of key proteins within this pathway such as Wnt and Frizzled to modulate gene expression. It also interacts with proteins like beta-catenin which are pivotal to these signaling cascades therefore contributing to tissue specification and differentiation processes.

WISP3 mutations are associated with Progressive Pseudorheumatoid Dysplasia (PPD) and have connections to conditions affecting skeletal development. PPD is a disorder characterized by cartilage degeneration leading to joint pain and stiffness. WISP3's interaction with IGF-related proteins is critical in these conditions as dysregulation can lead to improper tissue development and homeostasis. Studies also link abnormal WISP3 activity to osteoarthritis where its altered expression might contribute to the disease pathology by affecting cartilage cell signaling and matrix production.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mitochondrial electron transport and mitochondrial respiration (PubMed : 27252383). Through its regulation of the mitochondrial function may play a role in normal postnatal skeletal growth and cartilage homeostasis (PubMed : 10471507, PubMed : 27252383).
See full target information CCN6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research and treatment 172:577-586 PubMed30220054

2018

CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast.

Applications

Unspecified application

Species

Unspecified reactive species

Emily R McMullen,Maria E Gonzalez,Stephanie L Skala,Mai Tran,Dafydd Thomas,Sabra I Djomehri,Boris Burman,Kelley M Kidwell,Celina G Kleer
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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