Rabbit Polyclonal WNK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNK1 aa 1050-1300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Or Tris-EDTA buffer pH8.0 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Serine/threonine-protein kinase component of the WNK1-SPAK/OSR1 kinase cascade, which acts as a key regulator of blood pressure and regulatory volume increase by promoting ion influx (PubMed:15883153, PubMed:17190791, PubMed:31656913, PubMed:34289367, PubMed:36318922). WNK1 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed:36318922). The membraneless compartment concentrates WNK1 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK1-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (PubMed:15883153, PubMed:16263722, PubMed:17190791, PubMed:19739668, PubMed:21321328, PubMed:22989884, PubMed:25477473, PubMed:34289367, PubMed:36318922). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A5/KCC2 and SLC12A6/KCC3, regulating their activity (PubMed:16263722, PubMed:21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed:19665974, PubMed:21321328). Also acts as a regulator of angiogenesis in endothelial cells via activation of OXSR1/OSR1 and STK39/SPAK: activation of OXSR1/OSR1 regulates chemotaxis and invasion, while STK39/SPAK regulates endothelial cell proliferation (PubMed:25362046). Also acts independently of the WNK1-SPAK/OSR1 kinase cascade by catalyzing phosphorylation of other substrates, such as SYT2, PCF11 and NEDD4L (PubMed:29196535). Mediates phosphorylation of SYT2, regulating SYT2 association with phospholipids and membrane-binding (By similarity). Regulates mRNA export in the nucleus by mediating phosphorylation of PCF11, thereby decreasing the association between PCF11 and POLR2A/RNA polymerase II and promoting mRNA export to the cytoplasm (PubMed:29196535). Acts as a negative regulator of autophagy (PubMed:27911840). Required for the abscission step during mitosis, independently of the WNK1-SPAK/OSR1 kinase cascade (PubMed:21220314). May also play a role in actin cytoskeletal reorganization (PubMed:10660600). Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels, such as SLC4A4, SLC26A6, SLC26A9, TRPV4 and CFTR (By similarity). Involved in the regulation of epithelial Na(+) channel (ENaC) by promoting activation of SGK1 in a kinase-independent manner: probably acts as a scaffold protein that promotes the recruitment of SGK1 to the mTORC2 complex in response to chloride, leading to mTORC2-dependent phosphorylation and activation of SGK1 (PubMed:36373794). Acts as an assembly factor for the ER membrane protein complex independently of its protein kinase activity: associates with EMC2 in the cytoplasm via its amphipathic alpha-helix, and prevents EMC2 ubiquitination and subsequent degradation, thereby promoting EMC2 stabilization (PubMed:33964204).Isoform 3Kinase-defective isoform specifically expressed in kidney, which acts as a dominant-negative regulator of the longer isoform 1 (PubMed:14645531). Does not directly inhibit WNK4 and has no direct effect on sodium and chloride ion transport (By similarity). Down-regulates sodium-chloride cotransporter activity indirectly by inhibiting isoform 1, it associates with isoform 1 and attenuates its kinase activity (By similarity). In kidney, may play an important role regulating sodium and potassium balance (By similarity).
HSN2, KDP, KIAA0344, PRKWNK1, WNK1, Serine/threonine-protein kinase WNK1, Erythrocyte 65 kDa protein, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, p65, hWNK1
Rabbit Polyclonal WNK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNK1 aa 1050-1300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
WNK1 also known as With No Lysine 1 is a protein kinase with a molecular mass of approximately 251 kDa. Mechanically WNK1 functions as a serine/threonine kinase influencing the phosphorylation status of various substrates involved in ionic balance and cell volume regulation. It plays an important role in controlling ion transport channels. The protein is expressed in multiple tissues with high levels in the kidney and lower levels in the brain heart and other regions of the body. Its activity is especially important in the distal nephrons of the kidney.
WNK1 affects sodium and potassium ion transport which is essential for maintaining homeostasis. It is a part of a larger signaling complex where it modulates the activity of SPAK and OSR1 kinases which directly affect ion transporters like NKCCs and NCC. By regulating these kinases WNK1 contributes to maintaining the balance of electrolytes influencing blood pressure and general cellular osmoregulation.
WNK1 significantly impacts the WNK-SPAK/OSR1 signaling pathway which has direct roles in sodium and potassium ion transport. The pathway associates closely with proteins like SPAK and OSR1 which WNK1 activates affecting the function of ion transporters and channels. This signaling mechanism plays an integral role in renal salt reabsorption and is important for controlling systemic blood pressure.
Mutations and dysregulation of WNK1 link to hereditary hypertension and pseudohypoaldosteronism type II (PHAII) also known as Gordon's syndrome. These conditions involve elevated blood pressure and electrolyte imbalance due to altered transport activity. A change in WNK1 function can disrupt normal interaction with other proteins in its pathway such as NCC leading to these disorders. Understanding WNK1's role offers potential therapeutic targets for managing conditions related to electrolyte imbalance and blood pressure regulation.
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5% SDS PAGE
All lanes: Western blot - Anti-WNK1 antibody (ab137687) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 250 kDa
Immunofluorescence analysis of methanol-fixed HeLa cells labelling WNK1 with ab137687 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human H1299 xenograft tissue labelling WNK1 with ab137687 at 1/100 dilution.
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