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AB239037

Anti-WNK2 antibody

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(2 Publications)

Rabbit Polyclonal WNK2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNK2 aa 1150 to C-terminus.

View Alternative Names

KIAA1760, PRKWNK2, SDCCAG43, P/OKcl.13, WNK2, Serine/threonine-protein kinase WNK2, Antigen NY-CO-43, Protein kinase lysine-deficient 2, Protein kinase with no lysine 2, Serologically defined colon cancer antigen 43

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK2 antibody (AB239037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK2 antibody (AB239037)

Paraffin-embedded human prostate tissue stained for WNK2 with ab239037 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-WNK2 antibody (AB239037)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WNK2 antibody (AB239037)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for WNK2 using ab239037 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK2 antibody (AB239037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK2 antibody (AB239037)

Paraffin-embedded human small intestine tissue stained for WNK2 with ab239037 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human WNK2 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3S1

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB260033

Anti-G-protein coupled receptor 30 antibody [EPR23701-79]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (AB260033)

  • 5
  • 1
Primary Antibodies

AB174854

Anti-WNK1 antibody [EPR2743(3)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody [EPR2743(3)] (AB174854)

  • 0
  • 0
Primary Antibodies

AB154942

Anti-THAP11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THAP11 antibody (AB154942)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WNK2 also known as With No Lysine (K) Kinase 2 is a serine/threonine kinase weighing approximately 250 kDa. It plays a critical role in regulating ion transport by modulating various ion channels and transporters. WNK2 is expressed in several tissues with higher expression in the central nervous system and some epithelial tissues. This kinase operates through interacting with other proteins and enzymes to facilitate its functional role in cellular processes.
Biological function summary

WNK2 contributes to cellular signaling and volume regulation. It does not form part of a complex itself but its function gets integrated through its interaction with pathways affecting cytoskeleton dynamics and cell survival. WNK2’s phosphorylation activity helps in reorganizing cellular components and aids cells in adapting to osmotic stress therefore supporting cellular homeostasis.

Pathways

Several interacting molecules highlight the significance of WNK2 in signal transduction. WNK2’s role involves the MAPK/ERK pathway where it cooperates with STE20 and other kinases to relay signals from the cell surface to the nucleus. This interaction influences cell proliferation and differentiation shedding light on WNK2's contribution to the regulation of growth signals. Another important pathway involving WNK2 is the regulation of sodium and potassium balance through its communication with related kinases like WNK1 and WNK4.

WNK2’s malfunction has links to conditions like hypertension and cancer. Abnormalities in WNK2 expression or activity can lead to altered cellular homeostasis and excessive cell proliferation which get commonly observed in various types of cancer. Dysregulation in WNK2 activity also relates to hypertension where it affects sodium and water retention partly through interaction with kinases such as WNK3. Understanding these connections can prove valuable in developing interventions targeting these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase component of the WNK2-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation (PubMed : 17667937, PubMed : 18593598, PubMed : 21733846). The WNK2-SPAK/OSR1 kinase cascade is composed of WNK2, which mediates phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (By similarity). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters, regulating their activity (By similarity). Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed : 21733846). Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5 (PubMed : 21733846). Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression (PubMed : 17667937, PubMed : 18593598). Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1 (PubMed : 17667937, PubMed : 18593598). WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity (PubMed : 17667937, PubMed : 18593598).
See full target information WNK2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:444-455 PubMed38169557

2024

CNKSR2 expression is correlated with immune infiltrates in Cervical Cancer as a favorable prognostic factor.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxiao Li,Qiaoqiao Chen,Shihao Hong,Xiaoli Yang,Xiaoling Liang,Jianhua Yang

Bioengineered 13:12030-12044 PubMed35549643

2022

microRNA-324-3p suppresses the aggressive ovarian cancer by targeting /RAS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjie Li,Zhen Liang,Yongqin Jia,Panyang Zhang,Kaijian Ling,Yanzhou Wang,Zhiqing Liang
View all publications
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