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AB187503

Anti-WNK3 antibody

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(1 Publication)

Rabbit Polyclonal WNK3 antibody. Suitable for WB and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human WNK3 aa 1000-1400.

View Alternative Names

KIAA1566, PRKWNK3, WNK3, Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3

1 Images
Western blot - Anti-WNK3 antibody (AB187503)
  • WB

Supplier Data

Western blot - Anti-WNK3 antibody (AB187503)

All lanes:

Western blot - Anti-WNK3 antibody (ab187503) at 1/500 dilution

Lane 1:

Rat hypothalamus extract

Lane 2:

Rat CNS extract

Lane 3:

Rat brain extract

Predicted band size: 192 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human WNK3 aa 1000-1400. The exact immunogen used to generate this antibody is proprietary information.

Q9BYP7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WNK3 also known as With No Lysine [K] kinase 3 is a serine/threonine-protein kinase weighing approximately 203 kDa. Its expression occurs in various tissues with notable presence in the kidney brain and liver. Structurally WNK3 contains several domains that interact with proteins involved in ion transport and signaling pathways. WNK3 executes its mechanical functions by influencing ion transporter activity such as by regulating the sodium-chloride cotransporter (NCC) and the sodium-potassium-chloride cotransporter (NKCC).
Biological function summary

WNK3 plays a critical role in cellular ion homeostasis and modulation of ion transporters in response to osmotic changes. It is part of a complex that regulates ion balance within cells affecting cell volume and blood pressure. Through phosphorylation activities WNK3 can activate or inhibit other kinases impacting cellular responses to various stimuli. These regulatory actions are significant for maintaining proper electrolyte balance and fluid dynamics in the body.

Pathways

WNK3 is involved in regulating the WNK-SPAK/OSR1 pathway which is essential in controlling ion transporter activities in cells. This pathway fine-tunes the activity of the NCC and NKCC transporters. WNK3 is closely connected with other kinases like WNK1 and WNK4 with all playing common roles in blood pressure regulation and ion transport. The coordination between these kinases ensures precise cellular responses to changes in osmolarity and extracellular signals.

WNK3's regulation of ion balance connects it to hypertension and congenital nephrologic disorders. Hypertension links to the dysregulation of electrolyte transport and blood pressure control influenced by WNK3. Furthermore mutations in WNK3 alongside related proteins like WNK1 and NCC can result in altered renal salt handling leading to congenital disorders such as pseudohypoaldosteronism type II. Understanding WNK3's function and interactions offers valuable insights into treatments targeting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (PubMed : 16275911, PubMed : 16275913, PubMed : 16501604, PubMed : 22989884, PubMed : 36318922). WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed : 36318922). The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (PubMed : 22989884). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 16275911, PubMed : 16275913). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux (PubMed : 16275911, PubMed : 16275913, PubMed : 16357011, PubMed : 19470686, PubMed : 21613606). Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC (PubMed : 17975670). May also phosphorylate NEDD4L (PubMed : 20525693). Also acts as a scaffold protein independently of its protein kinase activity : negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9 (PubMed : 16357011, PubMed : 17673510). Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed : 18768590).
See full target information WNK3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1157731 PubMed37006245

2023

Deficient leptin receptor signaling in T cells of human SLE.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liu,Ming Zheng,Li Jia,Mingyuan Wang,Longhai Tang,Zhenke Wen,Miaojia Zhang,Fenghong Yuan
View all publications
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