AB15251

Anti-Wnt1 antibody

Name: Anti-Wnt1 antibody (ab15251) | Abcam
Brand: Abcam Limited
SKU: AB15251
Price: 750 USD
Availability: InStock
Rating: 5 (8 reviews)

(8 Reviews)

(198 Publications)

Rabbit Polyclonal WNT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 198 publications.

View Alternative Names

INT1, WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB15251)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB15251)

Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);

Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (AB15251)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (AB15251)

ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB15251)

ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

Unknown

Western blot - Anti-Wnt1 antibody (AB15251)

All lanes:

Western blot - Anti-Wnt1 antibody (ab15251) at 1/25 dilution

All lanes:

NIH3T3 cell lysate

Predicted band size: 40 kDa

Observed band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt1 also known as Int-1 is a member of the Wnt gene family and is known for its role in encoding a protein that is involved in signaling pathways inside the cell. This protein weighs approximately 41 kDa and is expressed in various tissues including the brain and lungs. It functions by binding to receptors on the cell surface activating intracellular signaling pathways that govern cellular processes like proliferation and differentiation.

Biological function summary

Wnt1 plays a significant role in embryonic development. It is an integral component of the Wnt signaling complex which regulates important aspects such as cell fate determination patterning during embryogenesis and organogenesis. The protein's activity ensures that cells behave in a coordinated manner allowing for proper tissue development and growth.

Pathways

Wnt1 is essential in the canonical Wnt/β-catenin signaling pathway. This pathway involves the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus where it influences gene transcription. It also plays a role in the non-canonical Wnt pathways such as the planar cell polarity pathway but its primary activity is within the canonical one. Proteins like Frizzled and Dishevelled are closely related through these pathways facilitating the transduction of signals initiated by Wnt1.

Wnt1 has been linked to cancers including breast and colorectal cancer due to its role in regulating cell proliferation and survival. Dysregulation of Wnt1 signaling can lead to poor control over cell growth contributing to oncogenesis. Wnt1 is also associated with bone density disorders as it critically affects bone formation and remodeling. In these contexts proteins such as LRP5/6 and β-catenin interact with Wnt1 mediating its effects on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed : 23499309, PubMed : 23656646, PubMed : 26902720, PubMed : 28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed : 23499309, PubMed : 23656646).

See full target information WNT1

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Publications (198)

Recent publications for all applications. Explore the full list and refine your search

World journal of stem cells 17:109330 PubMed41025098

2025

Exercise combined with induced pluripotent stem cells enhances the Wnt1-Lmx1a loop in the midbrain of Parkinsonian mice to alleviate Parkinsonian symptoms.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Jiang,Zhi-Min Lu,Qing-Lu Wang,Ying Luo,Jie Zhang

Journal of gastrointestinal oncology 16:1550-1561 PubMed40950339

2025

Andrographolide potentiates anti-tumor immunity in colorectal cancer (CRC) by targeting voltage-dependent anion channel (VDAC) and activating the cGAS-STING axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaming Wu,Ben Li,Yutong Chen,Mili Zhang,Jin Li,Guangjian Dou,Yuping Peng,Liyong Huang,Yan Zhou,Zhiheng Chen

Experimental and therapeutic medicine 30:208 PubMed40927640

2025

Tetramethylpyrazine alleviates acute kidney injury by activating the Wnt/β-catenin pathway independent of DKK1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wang,Xiaoxia Chang,Donglin Yang,Lixia Zhang,Zijie Guo,Xuhong Sun,Aiqun Li,Yanbo Ni,Pengchao Du

Diabetes & metabolism journal : PubMed40890020

2025

E2F5 Accelerates Vascular Smooth Muscle Cells Phenotype Switching in Diabetic Atherosclerosis through Activating Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxue Di,Jie Wang,Lin Sun,Guang Yang,Qun Xu

Scientific reports 15:29484 PubMed40796594

2025

Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Zhang,Yu Jin,Qiannan Ding,Yulei Li,Lixia Yang,Mengyun Li,Zuo Lv,Kaifang Wang,Maowei Yang,Haixin Zhang,Xiucheng Li

International journal of molecular sciences 26: PubMed40430063

2025

Exploring the Bone-Liver Axis: Impact of Acute Ethanol Intoxication on Post-Traumatic Liver Inflammation and Damage Following Femur Fracture.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Maria Bülow,Helen Rinderknecht,Nils Becker,Kernt Köhler,Alessa Wagner,Yuntao Yang,Katrin Bundkirchen,Claudia Neunaber,Borna Relja

Journal of translational medicine 23:137 PubMed39885534

2025

Usp7 contributes to the tail regeneration of planarians via Islet/Wnt1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ang Liang,Jinglong Liu,Zhiyuan Zhang,Jing Xiao,Dezeng Liu,Zimei Dong,Guangwen Chen

Iranian journal of basic medical sciences 28:130-139 PubMed39877631

2025

Echinacoside alleviates Ang II-induced cardiac fibrosis by enhancing the SIRT1/IL-11 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingbiao Wu,Zhongping Ning

Dose-response : a publication of International Hormesis Society 22:15593258241298045 PubMed39506979

2024

Salidroside Pre-Treatment Inhibits Hypertensive Renal Injury and Fibrosis Through Inhibiting Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhu,Liang Li,Yuting Luan,Ziqing Zhang,Yi Wang,Zhenyu Xu

Materials today. Bio 29:101300 PubMed39469313

2024

Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Tongmeng Jiang,Juan Wang,Hongping Ge,Yaqi Zhang,Tong Li,Chen Wang,Weiguo Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB15251)

Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (AB15251)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB15251)

Western blot - Anti-Wnt1 antibody (AB15251)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (198)

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