Rabbit Polyclonal WNT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 155 publications.
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Publications
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- BMC cancer 23:11282023LMP2 and TAP2 impair tumor growth and metastasis by inhibiting Wnt/β-catenin signaling pathway and EMT in cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengyan Cheng,Hongbo Wang,Zewei Yang,Jiaxu Li,Xing ChenPubMed 37986152
- International journal of rheumatic diseases 26:1547-15562023Astragalosides inhibit proliferation of fibroblast-like synoviocytes in experimental arthritis by modulating LncRNA S56464.1/miR-152-3p/Wnt1 signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoya Cui,Jing Wang,Chang Fan,Hui Jiang,Weiping LiPubMed 37317788
- International journal of molecular sciences 24:2023Association of Membranous WNT-1 and Nuclear mTOR with Endometrial Cancer Grade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilosz Pietrus,Kazimierz Pitynski,Marcin Waligora,Katarzyna Milian-Ciesielska,Artur Ludwin,Maciej W Socha,Klaudia SkrzypekPubMed 37176048
- Proceedings of the National Academy of Sciences of the United States of America 120:e22182381202023Structure of the Wnt-Frizzled-LRP6 initiation complex reveals the basis for coreceptor discrimination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaotaka Tsutsumi,Sunhee Hwang,Deepa Waghray,Simon Hansen,Kevin M Jude,Nan Wang,Yi Miao,Caleb R Glassman,Nathanael A Caveney,Claudia Y Janda,Rami N Hannoush,K Christopher GarciaPubMed 36893265
- European journal of medical research 28:842023Pro-fibrotic and apoptotic activities of circARAP1 in myocardial ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Li,Lei Guo,Jingjing Wang,Xing YangPubMed 36803446
- Immunity, inflammation and disease 11:e7442023Knockdown of LINC01138 protects human chondrocytes against IL-1β-induced damage by regulating the hsa-miR-1207-5p/KIAA0101 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiangtao Zhang,Genbing LvPubMed 36705420
- American journal of cancer research 12:5552-55632022Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingxiu Xu,Mingjie Chen,Mingyu Hu,Hanlu Wang,Zhongqiang Zuo,Jianguo Wang,Zhiyin XiePubMed 36628278
- Journal of oral biology and craniofacial research 12:721-7262022Amelogenin as a regenerative endodontic molecule for immature teeth with apical periodontitis. An experimental study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaha M F Mounir,Fatma M Rashed,Sahar M BukharyPubMed 36097610
- BMC endocrine disorders 22:1892022Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaopeng Huang,Siyun Li,Wenjie Lu,Longjiang XiongPubMed 35869471
- Bioengineered 13:12876-128872022MicroRNA-582-3p targeting ribonucleotide reductase regulatory subunit M2 inhibits the tumorigenesis of hepatocellular carcinoma by regulating the Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Xu,Bin LiPubMed 35609318
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Expected |
| Mouse | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed:23499309, PubMed:23656646, PubMed:26902720, PubMed:28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed:23499309, PubMed:23656646).
Alternative names
INT1, WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
Recommended products
Rabbit Polyclonal WNT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 155 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt1 also known as Int-1 is a member of the Wnt gene family and is known for its role in encoding a protein that is involved in signaling pathways inside the cell. This protein weighs approximately 41 kDa and is expressed in various tissues including the brain and lungs. It functions by binding to receptors on the cell surface activating intracellular signaling pathways that govern cellular processes like proliferation and differentiation.
- Biological function summary
Wnt1 plays a significant role in embryonic development. It is an integral component of the Wnt signaling complex which regulates important aspects such as cell fate determination patterning during embryogenesis and organogenesis. The protein's activity ensures that cells behave in a coordinated manner allowing for proper tissue development and growth.
- Pathways
Wnt1 is essential in the canonical Wnt/β-catenin signaling pathway. This pathway involves the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus where it influences gene transcription. It also plays a role in the non-canonical Wnt pathways such as the planar cell polarity pathway but its primary activity is within the canonical one. Proteins like Frizzled and Dishevelled are closely related through these pathways facilitating the transduction of signals initiated by Wnt1.
- Associated diseases and disorders
Wnt1 has been linked to cancers including breast and colorectal cancer due to its role in regulating cell proliferation and survival. Dysregulation of Wnt1 signaling can lead to poor control over cell growth contributing to oncogenesis. Wnt1 is also associated with bone density disorders as it critically affects bone formation and remodeling. In these contexts proteins such as LRP5/6 and β-catenin interact with Wnt1 mediating its effects on disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Wnt1 antibody (ab15251)
All lanes: Western blot - Anti-Wnt1 antibody (ab15251) at 1/25 dilution
All lanes: NIH3T3 cell lysate
Predicted band size: 40 kDa
Observed band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)
ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (ab15251)
ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)
Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
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