AB189001

Anti-Wnt1 antibody

Name: Anti-Wnt1 antibody (ab189001) | Abcam
Brand: Abcam Limited
SKU: AB189001
Price: 630 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal WNT1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human WNT1.

View Alternative Names

INT1, WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB189001)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB189001)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Wnt1 with ab189001 at 5 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human WNT1. The exact immunogen used to generate this antibody is proprietary information.

P04628

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt1 also known as Int-1 is a member of the Wnt gene family and is known for its role in encoding a protein that is involved in signaling pathways inside the cell. This protein weighs approximately 41 kDa and is expressed in various tissues including the brain and lungs. It functions by binding to receptors on the cell surface activating intracellular signaling pathways that govern cellular processes like proliferation and differentiation.

Biological function summary

Wnt1 plays a significant role in embryonic development. It is an integral component of the Wnt signaling complex which regulates important aspects such as cell fate determination patterning during embryogenesis and organogenesis. The protein's activity ensures that cells behave in a coordinated manner allowing for proper tissue development and growth.

Pathways

Wnt1 is essential in the canonical Wnt/β-catenin signaling pathway. This pathway involves the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus where it influences gene transcription. It also plays a role in the non-canonical Wnt pathways such as the planar cell polarity pathway but its primary activity is within the canonical one. Proteins like Frizzled and Dishevelled are closely related through these pathways facilitating the transduction of signals initiated by Wnt1.

Wnt1 has been linked to cancers including breast and colorectal cancer due to its role in regulating cell proliferation and survival. Dysregulation of Wnt1 signaling can lead to poor control over cell growth contributing to oncogenesis. Wnt1 is also associated with bone density disorders as it critically affects bone formation and remodeling. In these contexts proteins such as LRP5/6 and β-catenin interact with Wnt1 mediating its effects on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed : 23499309, PubMed : 23656646, PubMed : 26902720, PubMed : 28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed : 23499309, PubMed : 23656646).

See full target information WNT1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928134

2024

Evaluation of the Canonical Wnt Signaling Pathway in the Hearts of Hypertensive Rats of Various Etiologies.

Applications

Unspecified application

Species

Unspecified reactive species

Maryla Anna Młynarczyk,Natalia Domian,Irena Kasacka

American journal of translational research 15:5047-5070 PubMed37692966

2023

Matrine improves the hepatic microenvironment and reverses epithelial-mesenchymal transition in hepatocellular carcinoma by inhibiting the Wnt-1/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfei Shi,Hua Zhang,Guangjie Han,Jinfeng Wang,Xin Han,Man Zhao,Xiaoling Duan,Lili Mi,Ning Li,Xiaolei Yin,Cuizhen Li,Fei Yin

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 64:57-63 PubMed37128792

2023

Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Caner Ozbey,Isil Aydemir

Pathology oncology research : POR 27:622733 PubMed34257587

2021

Estrogen Aggravates Tumor Growth in a Diffuse Gastric Cancer Xenograft Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyi Lee,Kyoung Mee Kim,Seung Yeon Lee,Joohee Jung

View all publications

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Product promise

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Anti-Wnt1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB189001)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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