AB63934

Anti-Wnt1 antibody

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(10 Publications )

Rabbit Polyclonal WNT1 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human WNT1.

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Western blot - Anti-Wnt1 antibody (AB63934)

All lanes:

Western blot - Anti-Wnt1 antibody (ab63934) at 1 µg/mL

All lanes:

Jurkat cell lysate at 35 µg

Predicted band size: 40 kDa

Observed band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human WNT1. The exact immunogen used to generate this antibody is proprietary information.

P04628

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3571 Anti-Wnt-1 Antibody. 3571-100 is the same size as the 100 µg size of ab63934.

Wnt1 antibody has been purified by Protein A/G combination.

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt1 also known as Int-1 is a member of the Wnt gene family and is known for its role in encoding a protein that is involved in signaling pathways inside the cell. This protein weighs approximately 41 kDa and is expressed in various tissues including the brain and lungs. It functions by binding to receptors on the cell surface activating intracellular signaling pathways that govern cellular processes like proliferation and differentiation.

Biological function summary

Wnt1 plays a significant role in embryonic development. It is an integral component of the Wnt signaling complex which regulates important aspects such as cell fate determination patterning during embryogenesis and organogenesis. The protein's activity ensures that cells behave in a coordinated manner allowing for proper tissue development and growth.

Pathways

Wnt1 is essential in the canonical Wnt/β-catenin signaling pathway. This pathway involves the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus where it influences gene transcription. It also plays a role in the non-canonical Wnt pathways such as the planar cell polarity pathway but its primary activity is within the canonical one. Proteins like Frizzled and Dishevelled are closely related through these pathways facilitating the transduction of signals initiated by Wnt1.

Wnt1 has been linked to cancers including breast and colorectal cancer due to its role in regulating cell proliferation and survival. Dysregulation of Wnt1 signaling can lead to poor control over cell growth contributing to oncogenesis. Wnt1 is also associated with bone density disorders as it critically affects bone formation and remodeling. In these contexts proteins such as LRP5/6 and β-catenin interact with Wnt1 mediating its effects on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed : 23499309, PubMed : 23656646, PubMed : 26902720, PubMed : 28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed : 23499309, PubMed : 23656646).

See full target information WNT1

Alternative Names

INT1, WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 104:e70012 PubMed39487106

2024

Mitotic Arrest Deficient 2 Like 1 Contributes to Colorectal Cancer Cell Migration, Invasion, and Oxaliplatin Resistance Through the Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ding,Yonggui Zhou,Linfang He,Hubin Kang,Youwu Wen,Jia Xu,Congbo Zhu,Libing Luo,Qingjun Zeng

Renal failure 44:191-203 PubMed35170385

2022

MiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yirong Liu,Zhihong Ma,Zhenxing Huang,Dongmei Zou,Junbin Li,Ping Feng

Science advances 8:eabl5613 PubMed35044823

2022

Loss of IRF2BPL impairs neuronal maintenance through excess Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Paul C Marcogliese,Debdeep Dutta,Shrestha Sinha Ray,Nghi D P Dang,Zhongyuan Zuo,Yuchun Wang,Di Lu,Fatima Fazal,Thomas A Ravenscroft,Hyunglok Chung,Oguz Kanca,JiJun Wan,Emilie D Douine,Undiagnosed Diseases Network,Loren D M Pena,Shinya Yamamoto,Stanley F Nelson,Matthew Might,Kathrin C Meyer,Nan Cher Yeo,Hugo J Bellen

Experimental and therapeutic medicine 22:1137 PubMed34466147

2021

Role of lncRNA BCYRN1 in trophoblast cell physiology and pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Chen,Qi Shi,Bo Fan,Yan Cai

International journal of medical sciences 18:2814-2827 PubMed34220309

2021

Abnormal proinflammatory and stressor environmental with increased the regulatory cellular IGF-1/PAPP-A/STC and Wnt-1/β-Catenin canonical pathway in placenta of women with Chronic venous Disease during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Oscar Fraile-Martínez,Miguel A Saez,Miguel A Álvarez-Mon,Ana M Gómez-Lahoz,Coral Bravo,Juan A De León Luis,Felipe Sainz,Santiago Coca,Ángel Asúnsolo,Jorge Monserrat,Luis G Guijarro,Melchor Álvarez-Mon,Julia Bujan,Natalio García-Honduvilla

International journal of molecular medicine 47: PubMed33448322

2021

lncRNAS56464.1 as a ceRNA promotes the proliferation of fibroblast‑like synoviocytes in experimental arthritis via the Wnt signaling pathway and sponges miR‑152‑3p.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Jiang,Jian Liu,Chang Fan,Jing Wang,Weiping Li

Bioscience reports 40: PubMed32914833

2020

Therapeutic potential of Liuwei Dihuang pill against KDM7A and Wnt/β-catenin signaling pathway in diabetic nephropathy-related osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Ming Liu,Rui Dong,Zhen Hua,Nan Ning Lv,Yong Ma,Gui Cheng Huang,Jian Cheng,Hai Yan Xu

Cell biology international 44:2075-2085 PubMed32652867

2020

Knockdown of DNA-binding protein A enhances the chemotherapy sensitivity of colorectal cancer via suppressing the Wnt/β-catenin/Chk1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Tong,Kai Qu,Guorong Wang,Ruiting Liu,Baojun Duan,Xiaoqiang Wang,Chang Liu

Journal of cellular biochemistry 120:16840-16852 PubMed31144388

2019

PRC1 gene silencing inhibits proliferation, invasion, and angiogenesis of retinoblastoma cells through the inhibition of the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jun Liao,Xiao-Long Yin,Yan Deng,Xiao-Wei Peng

Science signaling 4:ra71 PubMed22045851

2011

p53 and microRNA-34 are suppressors of canonical Wnt signaling.

Applications

Species

Unspecified reactive species

Nam Hee Kim,Hyun Sil Kim,Nam-Gyun Kim,Inhan Lee,Hyung-Seok Choi,Xiao-Yan Li,Shi Eun Kang,So Young Cha,Joo Kyung Ryu,Jung Min Na,Changbum Park,Kunhong Kim,Sanghyuk Lee,Barry M Gumbiner,Jong In Yook,Stephen J Weiss

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Wnt1 antibody

Western blot - Anti-Wnt1 antibody (AB63934)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (10)

Product promise