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AB106522

Anti-Wnt10a antibody

5

(1 Review)

|

(17 Publications)

Rabbit Polyclonal Wnt10a antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-10a.

View Alternative Names

Protein Wnt-10a, WNT10A

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)
  • IHC-P

AbReview32501****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)

Immunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.

Tissue was fixed with formalin and blocked with 5000 μg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-Wnt10a antibody (AB106522)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Wnt10a antibody (AB106522)

Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)

Paraffin-embedded mouse skeletal muscle tissue stained for Wnt10a using ab106522 at 5 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (AB106522)

Paraffin-embedded human skeletal muscle tissue stained for Wnt10a using ab106522 at 10 μg/ml in immunohistochemical analysis.

Western blot - Anti-Wnt10a antibody (AB106522)
  • WB

Unknown

Western blot - Anti-Wnt10a antibody (AB106522)

Lane 1:

Western blot - Anti-Wnt10a antibody (ab106522) at 1 µg/mL

Lane 2:

Western blot - Anti-Wnt10a antibody (ab106522) at 2 µg/mL

All lanes:

RAW264.7 cell lysate at 15 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human Protein Wnt-10a. The exact immunogen used to generate this antibody is proprietary information.

Q9GZT5

Specificity

Despite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p><strong>Not validated in human.</strong></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt10a also known as Wingless-type MMTV integration site family member 10A is a protein that plays an important role in cellular signaling. This protein has a molecular mass of approximately 46 kDa. Wnt10a is expressed in various tissues including skin teeth and hair follicles where it is involved in mediating several cellular processes. The protein participates in signaling events by binding to specific receptors on the cell surface initiating a cascade of intracellular activities that influence development and cell differentiation.
Biological function summary

The Wnt10a protein is involved in the regulation of development and differentiation of ectodermal organs. It acts as a ligand in the Wnt signaling pathway which is a critical component of developmental processes. Wnt10a does not form complexes with others but binds to Frizzled receptors on the cell surface to propagate signals inside the cell. These signaling events guide proper formation and functioning of skin hair teeth and certain other structures influencing tissue maintenance and growth.

Pathways

Wnt10a plays a major role in the Wnt signaling pathways specifically the canonical Wnt/β-catenin pathway. This pathway is essential for gene transcription regulation and Wnt10a activates this through interaction with Frizzled and LRP co-receptors. Wnt10a's function is particularly related to the control of gene expression necessary for embryonic development and cell proliferation often working alongside other Wnt family proteins such as Wnt10b to regulate these pathways.

Wnt10a has associations with ectodermal dysplasia and tooth agenesis. Mutations in the Wnt10a gene can result in developmental disorders affecting skin hair and teeth due to its role in ectodermal organ formation. Additionally reduced Wnt10a function is linked with conditions such as osteoporosis where bone density is compromised. The protein’s interaction with other Wnt proteins in these disorders highlights the intricate balance of the Wnt signaling network in maintaining tissue and organ health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays a role in normal ectoderm development (PubMed : 17847007, PubMed : 28589954). Required for normal tooth development (PubMed : 17847007, PubMed : 28589954, PubMed : 29178643). Required for normal postnatal development and maintenance of tongue papillae and sweat ducts (PubMed : 28589954). Required for normal proliferation of basal cells in tongue filiform papillae, plantar epithelium and sweat ducts. Required for normal expression of keratins in tongue papillae (By similarity). Required for normal expression of KRT9 in foot plant epithelium (PubMed : 28589954). Required for normal hair follicle function (PubMed : 28589954).
See full target information Protein Wnt-10a
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14109 PubMed38372175

2024

Spatially resolved transcriptome of the aging mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wu,Tianxiang Tu,Mingzhe Xie,Yiting Wang,Biao Yan,Yajun Gong,Jiayi Zhang,Xiaolai Zhou,Zhi Xie

International journal of molecular medicine 53: PubMed38240085

2024

NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhou,Qun Zhang,Chenyang Liu,Jiahao Fan,Wen Huang,Nan Li,Mingxia Yang,Hong Wang,Weiping Xie,Hui Kong

Biomolecules 13: PubMed37189443

2023

Collagen Crosslinking for Keratoconus: Cellular Signaling Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Karamichos,Sarah E Nicholas,Asher Khan,Kamran M Riaz

Endocrine connections 11: PubMed35904223

2022

Wnt/β-catenin signaling in the adrenal glands of rats in various types of experimental hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Irena Kasacka,Żaneta Piotrowska,Natalia Domian,Alicja Lewandowska

Stem cell research & therapy 13:279 PubMed35765096

2022

Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yang,Wei Sun,Xiaoyan Jing,Qian Zhang,Hui Huang,Zuojun Xu

Frontiers in oncology 12:871788 PubMed35494062

2022

Diffuse Large B-Cell Lymphoma Promotes Endothelial-to-Mesenchymal Transition WNT10A/Beta-Catenin/Snail Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xianting Sun,Jianchen Fang,Fen Ye,Shuxian Zhang,Honghui Huang,Jian Hou,Ting Wang

The European journal of neuroscience 54:7409-7421 PubMed34618385

2021

Wnt10a/β-catenin signalling is involved in kindlin-1-mediated astrocyte activation in a chronic construction injury rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Baisong Zhao,Yongying Pan,Haiping Xu,Xingrong Song

Cancer management and research 12:12375-12384 PubMed33299350

2020

MicroRNA-361-3p Inhibit the Progression of Lymphoma by the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhou,Huifeng Tang,Ning Li,Hang Chen,Xiaohui Chen,Lei Gu,Liang Zhang,Guoyan Tian,Diehong Tao

Journal of thoracic disease 12:5906-5915 PubMed33209423

2020

WNT/β-catenin pathway activation via Wnt1 overexpression and Axin1 downregulation correlates with cadherin-catenin complex disruption and increased lymph node involvement in micropapillary-predominant lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhu,Shifeng Yang,Linfeng Zheng,Gu Zhang,Guoping Cheng

Frontiers in oncology 10:566637 PubMed33330038

2020

Comparative Assessment of the WNT/β-Catenin Pathway, CacyBP/SIP, and the Immunoproteasome Subunit LMP7 in Various Histological Types of Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Żaneta Piotrowska,Michał Niezgoda,Grzegorz Młynarczyk,Magdalena Acewicz,Irena Kasacka
View all publications
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