Anti-Wnt10b antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Wnt10b antibody (AB70816)

ICC/IF image of ab70816 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70816, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10b antibody (AB70816)

ab70816 at 2.5 μ/ml staining Wnt10 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

• WB

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Western blot - Anti-Wnt10b antibody (AB70816)

Lane 1:

Western blot - Anti-Wnt10b antibody (ab70816) at 2 µg/mL

Lane 2:

Western blot - Anti-Wnt10b antibody (ab70816) at 4 µg/mL

All lanes:

Human skeletal muscle tissue lysate at 15 µg

Predicted band size: 43 kDa

Observed band size: 47 kDa,93 kDa

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