Rabbit Polyclonal Wnt10b antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human WNT10B.
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Publications
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- Frontiers in oncology 11:8082912022KB-68A7.1 Inhibits Hepatocellular Carcinoma Development Through Binding to NSD1 and Suppressing Wnt/β-Catenin Signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuhua Zhang,Jianqun Xu,Huan Cao,Mi Jiang,Jun XiongPubMed 35127520
- Cancer cell international 21:6512021CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Yang,Xin Fan,Yunfei Nie,Donglei Liu,Dengyan Zhu,Kai Wu,Yuan Zhang,Wenhua Li,Xiangyu Tian,Huaqi Wang,Yuxia FanPubMed 34872582
- Experimental and therapeutic medicine 22:13172021Gigantol inhibits cell proliferation and induces apoptosis by regulating DEK in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxing Cai,Yi Hao,Hui Xu,Kai Chen,Baozhong RenPubMed 34630671
- Clinical, cosmetic and investigational dermatology 14:527-5392021Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Zhai,Meihua Gong,Yixuan Peng,Daping YangPubMed 34040410
- Oncology reports 45:1083-10932021lncRNA HOTAIRM1 regulates cell proliferation and the metastasis of thyroid cancer by targeting Wnt10b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenyao Li,Xinxin Chen,Tao Liu,Guang ChenPubMed 33650656
- Frontiers in cell and developmental biology 8:6085442021PTEN Reduces BMP9-Induced Osteogenic Differentiation Through Inhibiting Wnt10b in Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFu-Shu Li,Pei-Pei Li,Ling Li,Yan Deng,Ying Hu,Bai-Cheng HePubMed 33614622
- BioMed research international 2020:94515962020Molecular Pathways Involved in Promoting Activity of Timosaponin BII on Hair Growth in C57BL/6 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Xiao et. al.PubMed 33015188
- Nucleic acids research 48:8870-88822020Extracellular vesicles engineered with valency-controlled DNA nanostructures deliver CRISPR/Cas9 system for gene therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialang Zhuang,Jizhou Tan,Chenglin Wu,Jie Zhang,Ting Liu,Chunhai Fan,Jiaping Li,Yuanqing ZhangPubMed 32810272
- Bioscience, biotechnology, and biochemistry 84:491-4992019Overexpressing of POU2F2 accelerates fracture healing via regulating HMGA1/Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Hanke Zhao,Kun Chen,Ye HuangPubMed 31782345
- Journal of experimental & clinical cancer research 38:4672019CRISPR/Cas9-mediated knockout of NSD1 suppresses the hepatocellular carcinoma development via the NSD1/H3/Wnt10b signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuhua Zhang et. al.PubMed 31727171
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WNT10B. The exact immunogen used to generate this antibody is proprietary information. Database link O00744
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Alternative names
WNT12, WNT10B, Protein Wnt-10b, Protein Wnt-12
Recommended products
Rabbit Polyclonal Wnt10b antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human WNT10B.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human WNT10B. The exact immunogen used to generate this antibody is proprietary information. Database link O00744
- Purification technique
- Affinity purification Immunogen
- Specificity
ab70816 only recognizes the longer isoform of Wnt10b and will not cross-react with Wnt10a.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt10b also known as wingless-related integration site 10b is a protein involved in signaling pathways critical for development and cellular processes. It has a molecular mass of approximately 46 kDa. This protein is mainly expressed in tissues like skin lung and adipose tissue. Wnt10b belongs to the broader Wnt family which is involved in mediating several signaling events during development.
- Biological function summary
Wnt10b influences cell fate decisions and plays a role in adipogenesis and osteogenesis. It is not standalone but part of the Wnt signaling complex involving interactions with other proteins such as Frizzled receptors and co-receptors like LRP5/6. Through these interactions Wnt10b influences the beta-catenin pathway impacting gene transcription and cellular differentiation.
- Pathways
Wnt10b plays roles in the Wnt/beta-catenin and Wnt/JNK pathways. These pathways control embryonic development and are important for tissue homeostasis. Wnt10b activates beta-catenin which partners with TCF/LEF transcription factors to modulate the expression of target genes. This protein shows functional similarity and connectivity with other Wnt family members such as Wnt3a which also engages in beta-catenin signaling.
- Associated diseases and disorders
Wnt10b has associations with obesity and osteoporosis. Elevated Wnt10b expression can inhibit adipogenesis linking it to potential obesity resistance. Conversely dysregulated Wnt signaling including Wnt10b may impair bone density and result in osteoporosis. The protein's interaction with LRP5 is significant in bone mass regulation highlighting Wnt10b's role in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-Wnt10b antibody (ab70816)
Lane 1: Western blot - Anti-Wnt10b antibody (ab70816) at 2 µg/mL
Lane 2: Western blot - Anti-Wnt10b antibody (ab70816) at 4 µg/mL
All lanes: Human skeletal muscle tissue lysate at 15 µg
Predicted band size: 43 kDa
Observed band size: 47 kDa, 93 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10b antibody (ab70816)
ab70816 at 2.5 μ/ml staining Wnt10 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunocytochemistry/ Immunofluorescence - Anti-Wnt10b antibody (ab70816)
ICC/IF image of ab70816 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70816, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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