JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317211

Anti-Wnt10b antibody [HL2370]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal Wnt10b antibody. Suitable for WB and reacts with Transfected cell lysate, Human, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human WNT10B.

View Alternative Names

WNT12, WNT10B, Protein Wnt-10b, Protein Wnt-12

3 Images
Western blot - Anti-Wnt10b antibody [HL2370] (AB317211)
  • WB

Supplier Data

Western blot - Anti-Wnt10b antibody [HL2370] (AB317211)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317211 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Wnt10b antibody [HL2370] (ab317211) at 1/500 dilution

Lane 1:

22RV1 whole cell extracts at 30 µg

Lane 2:

HCT116 whole cell extracts at 30 µg

Lane 3:

SK-MEL-5 whole cell extracts at 30 µg

Lane 4:

SH-SY5Y whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-Wnt10b antibody [HL2370] (AB317211)
  • WB

Supplier Data

Western blot - Anti-Wnt10b antibody [HL2370] (AB317211)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317211 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Wnt10b antibody [HL2370] (ab317211) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-Wnt10b antibody [HL2370] (AB317211)
  • WB

Supplier Data

Western blot - Anti-Wnt10b antibody [HL2370] (AB317211)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317211 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt10b antibody [HL2370] (ab317211) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Wnt1 transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

Wnt2 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Wnt2b transfected (+) 293T whole cell extracts at 30 µg

Lane 5:

Wnt3 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Wnt3a transfected (+) 293T whole cell extracts at 30 µg

Lane 7:

Wnt4 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Wnt5a transfected (+) 293T whole cell extracts at 30 µg

Lane 9:

Wnt6 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Wnt7a transfected (+) 293T whole cell extracts at 30 µg

Lane 11:

Wnt7b transfected (+) 293T whole cell extracts at 30 µg

Lane 12:

Wnt8a transfected (+) 293T whole cell extracts at 30 µg

Lane 13:

Wnt8b transfected (+) 293T whole cell extracts at 30 µg

Lane 14:

Wnt9a transfected (+) 293T whole cell extracts at 30 µg

Lane 15:

Wnt9b transfected (+) 293T whole cell extracts at 30 µg

Lane 16:

Wnt10a transfected (+) 293T whole cell extracts at 30 µg

Lane 17:

Wnt10b transfected (+) 293T whole cell extracts at 30 µg

Lane 18:

Wnt16 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2370

Isotype

IgG

Carrier free

No

Reacts with

Human, Zebrafish

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human WNT10B.

O00744

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt10b also known as wingless-related integration site 10b is a protein involved in signaling pathways critical for development and cellular processes. It has a molecular mass of approximately 46 kDa. This protein is mainly expressed in tissues like skin lung and adipose tissue. Wnt10b belongs to the broader Wnt family which is involved in mediating several signaling events during development.
Biological function summary

Wnt10b influences cell fate decisions and plays a role in adipogenesis and osteogenesis. It is not standalone but part of the Wnt signaling complex involving interactions with other proteins such as Frizzled receptors and co-receptors like LRP5/6. Through these interactions Wnt10b influences the beta-catenin pathway impacting gene transcription and cellular differentiation.

Pathways

Wnt10b plays roles in the Wnt/beta-catenin and Wnt/JNK pathways. These pathways control embryonic development and are important for tissue homeostasis. Wnt10b activates beta-catenin which partners with TCF/LEF transcription factors to modulate the expression of target genes. This protein shows functional similarity and connectivity with other Wnt family members such as Wnt3a which also engages in beta-catenin signaling.

Wnt10b has associations with obesity and osteoporosis. Elevated Wnt10b expression can inhibit adipogenesis linking it to potential obesity resistance. Conversely dysregulated Wnt signaling including Wnt10b may impair bone density and result in osteoporosis. The protein's interaction with LRP5 is significant in bone mass regulation highlighting Wnt10b's role in these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
See full target information WNT10B
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com