AB31962

Anti-Wnt11 antibody

Name: Anti-Wnt11 antibody (ab31962) | Abcam
Brand: Abcam Limited
SKU: AB31962
Price: 485 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(19 Publications)

Rabbit Polyclonal WNT11 antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 19 publications.

View Alternative Names

Protein Wnt-11, WNT11

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

ab31962 staining Wnt11 in HaCat cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31962 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Supplier Data

Western blot - Anti-Wnt11 antibody (AB31962)

Blocking Buffer : 2% BSA

All lanes:

Western blot - Anti-Wnt11 antibody (ab31962) at 1 µg/mL

All lanes:

Human Wnt11 Recombinant Protein at 0.01 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

This antibody detects recombinant human Wnt11 by Western Blotting. We have been unable to detect endogenous protein in cell lines with this antibody, possibly due to the low level of endogenous protein in the samples tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

See full target information WNT11

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:13331 PubMed37587207

2023

Hedyotis diffusa-Sculellaria barbata (HD-SB) suppresses the progression of colorectal cancer cells via the hsa_circ_0039933/hsa-miR-204-5p/wnt11 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Danye Zhu,Shanmin Yuan,Cong Chen

The European respiratory journal : PubMed32859680

2020

Wnt5a and Wnt11 as acute respiratory distress syndrome biomarkers for SARS-CoV-2 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Young Choi,Hee Ho Park,Hyelim Kim,Hong Nam Kim,Inyoung Kim,Soyoung Jeon,Wantae Kim,Jong-Sup Bae,Wonhwa Lee

The Journal of clinical investigation 130:4152-4166 PubMed32597829

2020

Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Wakako Kawarazaki,Risuke Mizuno,Mitsuhiro Nishimoto,Nobuhiro Ayuzawa,Daigoro Hirohama,Kohei Ueda,Fumiko Kawakami-Mori,Shigeyoshi Oba,Takeshi Marumo,Toshiro Fujita

Molecular medicine reports 22:1839-1846 PubMed32582982

2020

Tanshinone IIA promotes cardiac differentiation and improves cell motility by modulating the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Xiuyan Wang,Chenxing Fan,Chunxia Wu,Shizheng Li,Hua Liu

Cell reports 30:771-782.e6 PubMed31968252

2020

Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Ning Yin,Xue Wang,Andras Khoor,Vaishnavi Sambandam,Anwesha B Ghosh,Zoe A Fields,Nicole R Murray,Verline Justilien,Alan P Fields

Experimental and therapeutic medicine 18:4631-4636 PubMed31798700

2019

Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Chen,Shunen Xu,Wei Li,Lirong Wu,Long Wang,Yongkang Li,Wei Zhou

Cellular physiology and biochemistry : internation 49:2254-2263 PubMed30257243

2018

Hyperglycemia Altered the Fate of Cardiac Stem Cells to Adipogenesis through Inhibiting the β-Catenin/TCF-4 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Zhang,Ke Meng,Yujie Pu,Chao Wang,Yingying Chen,Linlin Wang

Journal of extracellular vesicles 7:1490144 PubMed30034643

2018

Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities.

Applications

Unspecified application

Species

Unspecified reactive species

C Spinelli,L Montermini,B Meehan,A R Brisson,S Tan,D Choi,I Nakano,J Rak

Biomedicine & pharmacotherapy = Biomedecine & phar 106:483-490 PubMed29990836

2018

MicroRNA-298 represses hepatocellular carcinoma progression by inhibiting CTNND1-mediated Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ningjia Cao,Liang Mu,Wei Yang,Li Liu,Liang Liang,Hong Zhang

Scientific reports 6:35562 PubMed27765945

2016

Different populations of Wnt-containing vesicles are individually released from polarized epithelial cells.

Applications

Species

Human

Qiuhong Chen,Ritsuko Takada,Chiyo Noda,Satoru Kobayashi,Shinji Takada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt11 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

Western blot - Anti-Wnt11 antibody (AB31962)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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