Rabbit Polyclonal WNT11 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT11 aa 1-300.
Images
Publications
Filter by
- Chemistry & biodiversity 20:e2022007682023Possible Mechanism of Dysphania ambrosioides (L.) Mosyakin & Clemants Seed Extract Suppresses the Migration and Invasion of Human Hepatocellular Carcinoma Cells SMMC-7721.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Huang,Junmei Hao,Jintao Nie,Ruihua Qian,Haiying Li,Jiayuan Zhao,Yanan WangPubMed 36694378
- Bone & joint research 10:558-5702021Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan Li,Zhi Peng,You Zhou,Yongyue Su,Pengfei Bu,Xuhan Meng,Bo Li,Yongqing XuPubMed 34465146
- Frontiers in oncology 7:922017Inhibitory Effect of 1,8-Cineol on β-Catenin Regulation, WNT11 Expression, and Cellular Progression in HNSCC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Roettger et. al.PubMed 28589081
- Stem cells (Dayton, Ohio) 32:1649-602014Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily C Keats,James M Dominguez,Maria B Grant,Zia A KhanPubMed 24496952
- Breast cancer research : BCR 14:R982012Lack of transforming growth factor-β signaling promotes collective cancer cell invasion through tumor-stromal crosstalk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren A Matise et. al.PubMed 22748014
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WNT11 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O96014
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Alternative names
Protein Wnt-11, WNT11
Recommended products
Rabbit Polyclonal WNT11 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT11 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WNT11 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O96014
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.
- Biological function summary
The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.
- Pathways
Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.
- Associated diseases and disorders
Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-Wnt11 antibody (ab96730)
10% SDS-PAGE
All lanes: Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
Paraffin-embedded mouse colon tissue stained Wnt11 using ab96730 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min
Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) using ab96730 at 1/200 dilution. Lower image merged with DNA probe.
Western blot - Anti-Wnt11 antibody (ab96730)
10% SDS-PAGE
All lanes: Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution
All lanes: Mouse heart tissue extractc at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)
Paraffin-embedded lung SCC xenograft satined Wnt11 using ab96730 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)
Immunofluorescence analysis of ice-cold MeOH-fixed (5 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) at cytoplasm using ab96730 at 1/500 dilution. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Western blot - Anti-Wnt11 antibody (ab96730)
10% SDS-PAGE
All lanes: Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution
All lanes: Mouse white adipose tissue extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
Western blot - Anti-Wnt11 antibody (ab96730)
10% SDS-PAGE
All lanes: Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution
All lanes: COLO 205 (human colon adenocarcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com