AB96730

Anti-Wnt11 antibody

Name: Anti-Wnt11 antibody (ab96730) | Abcam
Brand: Abcam Limited
SKU: AB96730
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal WNT11 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment, Rat, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT11 aa 1-300.

View Alternative Names

Protein Wnt-11, WNT11

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Paraffin-embedded lung SCC xenograft satined Wnt11 using ab96730 at 1/100 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) using ab96730 at 1/200 dilution. Lower image merged with DNA probe.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunofluorescence analysis of ice-cold MeOH-fixed (5 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) at cytoplasm using ab96730 at 1/500 dilution. Blue : Hoechst 33342 staining. Scale bar = 10 μm.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Paraffin-embedded mouse colon tissue stained Wnt11 using ab96730 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

10% SDS-PAGE

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

COLO 205 (human colon adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

COLO 205 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

Mouse heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/5000 dilution

All lanes:

Rat heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

true

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

10% SDS-PAGE

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/1000 dilution

All lanes:

Mouse white adipose tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody (AB96730)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt11 antibody (ab96730) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

WNT11 transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human WNT11 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O96014

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

See full target information WNT11

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Orphanet journal of rare diseases 20:289 PubMed40483479

2025

A de novo SALL4 mutation causes unilateral renal agenesis by misregulating genes involved in kidney development.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zhao,Yali Fan,Jieyan Li,Lin Li,Chenghong Yin

Chemistry & biodiversity 20:e202200768 PubMed36694378

2023

Possible Mechanism of Dysphania ambrosioides (L.) Mosyakin & Clemants Seed Extract Suppresses the Migration and Invasion of Human Hepatocellular Carcinoma Cells SMMC-7721.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Huang,Junmei Hao,Jintao Nie,Ruihua Qian,Haiying Li,Jiayuan Zhao,Yanan Wang

Bone & joint research 10:558-570 PubMed34465146

2021

Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Li,Zhi Peng,You Zhou,Yongyue Su,Pengfei Bu,Xuhan Meng,Bo Li,Yongqing Xu

The Kaohsiung journal of medical sciences 37:768-775 PubMed34002485

2021

Long non-coding RNA-H19 promotes ovarian cancer cell proliferation and migration via the microRNA-140/Wnt1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Wang,Wei-Jiao Gao

American journal of physiology. Cell physiology 317:C189-C199 PubMed31042421

2019

MicroRNA-140 inhibits skeletal muscle glycolysis and atrophy in endotoxin-induced sepsis in mice via the WNT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Tian-Mei Li,Xue-Ru Liu,Yi-Ping Bai,Jie Li,Ni Tang,Xiao-Bin Wang

Frontiers in oncology 7:92 PubMed28589081

2017

Inhibitory Effect of 1,8-Cineol on β-Catenin Regulation, WNT11 Expression, and Cellular Progression in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Roettger,Karl-Ludwig Bruchhage,Maren Drenckhan,Kirsten Ploetze-Martin,Ralph Pries,Barbara Wollenberg

Stem cells (Dayton, Ohio) 32:1649-60 PubMed24496952

2014

Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Emily C Keats,James M Dominguez,Maria B Grant,Zia A Khan

Breast cancer research : BCR 14:R98 PubMed22748014

2012

Lack of transforming growth factor-β signaling promotes collective cancer cell invasion through tumor-stromal crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren A Matise,Trenis D Palmer,William J Ashby,Abudi Nashabi,Anna Chytil,Mary Aakre,Michael W Pickup,Agnieszka E Gorska,Andries Zijlstra,Harold L Moses

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB96730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Western blot - Anti-Wnt11 antibody (AB96730)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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