Skip to main content

Rabbit Recombinant Monoclonal WNT11 antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Rat, Mouse samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Wnt11 antibody [EPR25633-141] (AB316738), expandable thumbnail
  • Western blot - Anti-Wnt11 antibody [EPR25633-141] (AB316738), expandable thumbnail
  • Western blot - Anti-Wnt11 antibody [EPR25633-141] (AB316738), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] (AB316738), expandable thumbnail
  • Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] (AB316738), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIPIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Expected
Expected
Not recommended
Not recommended
Rat
Tested
Expected
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Alternative names

Protein Wnt-11, WNT11

Recommended products

Rabbit Recombinant Monoclonal WNT11 antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25633-141
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Associated diseases and disorders

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738), expandable thumbnail

    Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738)

    Wnt11 Western blot staining using rabbit Anti-Wnt11 antibody

    Negative control: NCI-H1975 (PMID:31231131), A549 (PMID:31231131).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    Exposure time: Lane 1-2: 3 seconds, Lane 3-4: 26 seconds

    All lanes: Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738) at 1/1000 dilution

    Lane 1: TF-1 (human Erythroleukemia erythroblast) whole cell lysate at 60 µg with 5% NFDM/TBST

    Lane 2: NCI-H1975 (human adenocarcinoma lung epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST

    Lane 3: Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST

    Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 39 kDa, 36 kDa, 124 kDa

  • Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738), expandable thumbnail

    Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738)

    Wnt11 Western blot staining using rabbit Anti-Wnt11 antibody

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738) at 1/1000 dilution

    Lane 1: Untreated JEG-3 (human placenta epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST

    Lane 2: JEG-3 treated with 300 ng/ml Brefeldin A for 24h whole cell lysate at 60 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 39 kDa, 124 kDa

    Exposure time: 8s

  • Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738), expandable thumbnail

    Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738)

    Low expression: lung (PMID: 15084607).

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    Exposure time: Lane 1-2: 37 seconds, Lane 3-4: 26 seconds

    All lanes: Western blot - Anti-Wnt11 antibody [EPR25633-141] (ab316738) at 1/1000 dilution

    Lane 1: Rat fetal lung tissue lysate at 60 µg with 5% NFDM/TBST

    Lane 2: Rat lung tissue lysate at 60 µg with 5% NFDM/TBST

    Lane 3: Rat embryo tissue lysate at 60 µg with 5% NFDM/TBST

    Lane 4: Mouse embryo tissue lysate at 60 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 39 kDa, 15 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] (ab316738), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] (ab316738)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TF-1 (human erythroleukemia erythroblast) cells labelling Wnt11 with ab316738 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing cytoplamic staining in TF-1 cell line. Negative control: A549.

    Nuclear DNA was labeled with DAPI. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

  • Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] (ab316738), expandable thumbnail

    Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] (ab316738)

    Wnt11 was immunoprecipitated from 0.35 mg TF-1 (human Erythroleukemia erythroblast) whole cell lysate with ab316738 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316738 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: TF-1 (human Erythroleukemia erythroblast) whole cell lysate

    Lane 2: ab316738 IP in TF-1 (human Erythroleukemia erythroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316738 in TF-1 whole cell lysate

    All lanes: Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] (ab316738) at 1/30 dilution

    All lanes: TF-1 (human Erythroleukemia erythroblast) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 8s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com