Rabbit Recombinant Monoclonal WNT11 antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--western-blot-img428486.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739)")
![Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--western-blot-img428487.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739)")
![Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--western-blot-img428485.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739)")
![Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--immunocytochemistry-immunofluorescence-img428483.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739)")
![Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--immunoprecipitation-img428484.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (AB316739)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IP | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Expected | Expected | Not recommended | Not recommended |
| Rat | Tested | Expected | Expected | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Alternative names
Protein Wnt-11, WNT11
Recommended products
Rabbit Recombinant Monoclonal WNT11 antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25633-141
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316739 is the carrirer-free version of Anti-Wnt11 antibody [EPR25633-141] ab316738.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.
- Biological function summary
The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.
- Pathways
Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.
- Associated diseases and disorders
Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--western-blot-img428486.jpg "Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)")
Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)
Wnt11 Western blot staining using rabbit Anti-Wnt11 antibody
This data was developed using Anti-Wnt11 antibody [EPR25633-141] ab316738, the same antibody clone in a different buffer formulation.
Negative control: NCI-H1975 (PMID:31231131), A549 (PMID:31231131).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
Exposure time: Lane 1-2: 3 seconds, Lane 3-4: 26 seconds
All lanes: Western blot - Anti-Wnt11 antibody [EPR25633-141] (Anti-Wnt11 antibody [EPR25633-141] ab316738) at 1/1000 dilution
Lane 1: TF-1 (human Erythroleukemia erythroblast) whole cell lysate at 60 µg with 5% NFDM/TBST
Lane 2: NCI-H1975 (human adenocarcinoma lung epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST
Lane 3: Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST
Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 39 kDa, 36 kDa, 124 kDa
![Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--western-blot-img428487.jpg "Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)")
Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)
Wnt11 Western blot staining using rabbit Anti-Wnt11 antibody
This data was developed using Anti-Wnt11 antibody [EPR25633-141] ab316738, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Wnt11 antibody [EPR25633-141] (Anti-Wnt11 antibody [EPR25633-141] ab316738) at 1/1000 dilution
Lane 1: Untreated JEG-3 (human placenta epithelial cell) whole cell lysate at 60 µg with 5% NFDM/TBST
Lane 2: JEG-3 treated with 300 ng/ml Brefeldin A for 24h whole cell lysate at 60 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 39 kDa, 124 kDa
Exposure time: 8s
![Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--western-blot-img428485.jpg "Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)")
Western blot - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)
This data was developed using Anti-Wnt11 antibody [EPR25633-141] ab316738, the same antibody clone in a different buffer formulation.
Low expression: lung (PMID: 15084607).
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
Exposure time: Lane 1-2: 37 seconds, Lane 3-4: 26 seconds
All lanes: Western blot - Anti-Wnt11 antibody [EPR25633-141] (Anti-Wnt11 antibody [EPR25633-141] ab316738) at 1/1000 dilution
Lane 1: Rat fetal lung tissue lysate at 60 µg with 5% NFDM/TBST
Lane 2: Rat lung tissue lysate at 60 µg with 5% NFDM/TBST
Lane 3: Rat embryo tissue lysate at 60 µg with 5% NFDM/TBST
Lane 4: Mouse embryo tissue lysate at 60 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 39 kDa, 15 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--immunocytochemistry-immunofluorescence-img428483.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)")
Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)
This data was developed using Anti-Wnt11 antibody [EPR25633-141] ab316738, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TF-1 (human erythroleukemia erythroblast) cells labelling Wnt11 with Anti-Wnt11 antibody [EPR25633-141] ab316738 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing cytoplamic staining in TF-1 cell line. Negative control: A549.
Nuclear DNA was labeled with DAPI. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
![Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-epr25633-141-bsa-and-azide-free-ab316739--immunoprecipitation-img428484.jpg "Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)")
Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] - BSA and Azide free (ab316739)
This data was developed using Anti-Wnt11 antibody [EPR25633-141] ab316738, the same antibody clone in a different buffer formulation.
Wnt11 was immunoprecipitated from 0.35 mg TF-1 (human Erythroleukemia erythroblast) whole cell lysate with Anti-Wnt11 antibody [EPR25633-141] ab316738 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Wnt11 antibody [EPR25633-141] ab316738 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: TF-1 (human Erythroleukemia erythroblast) whole cell lysate
Lane 2: Anti-Wnt11 antibody [EPR25633-141] ab316738 IP in TF-1 (human Erythroleukemia erythroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Wnt11 antibody [EPR25633-141] ab316738 in TF-1 whole cell lysateAll lanes: Immunoprecipitation - Anti-Wnt11 antibody [EPR25633-141] (Anti-Wnt11 antibody [EPR25633-141] ab316738) at 1/30 dilution
All lanes: TF-1 (human Erythroleukemia erythroblast) whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
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