AB308177

Anti-Wnt11 antibody [HL1630]

Recombinant
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Rabbit Recombinant Monoclonal WNT11 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line, Recombinant fragment samples. Immunogen corresponding to Synthetic Fragment Protein within Human WNT11.

View Alternative Names

Protein Wnt-11, WNT11

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Supplier Data

Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody [HL1630] (ab308177) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt11 antibody [HL1630] (ab308177) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) 293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39.2 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt11 antibody [HL1630] (ab308177) at 1/1000 dilution

All lanes:

Caco-2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39.2 kDa

false

Supplier Data

Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)

All lanes:

Western blot - Anti-Wnt11 antibody [HL1630] (ab308177) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

Transfected 293T whole cell extract with Wnt1 at 30 µg

Lane 3:

Transfected 293T whole cell extract with Wnt2 at 30 µg

Lane 4:

Transfected 293T whole cell extract with Wnt3 at 30 µg

Lane 5:

Transfected 293T whole cell extract with Wnt3a at 30 µg

Lane 6:

Transfected 293T whole cell extract with Wnt4 at 30 µg

Lane 7:

Transfected 293T whole cell extract with Wnt5a at 30 µg

Lane 8:

Transfected 293T whole cell extract with Wnt6 at 30 µg

Lane 9:

Transfected 293T whole cell extract with Wnt7a at 30 µg

Lane 10:

Transfected 293T whole cell extract with Wnt7b at 30 µg

Lane 11:

Transfected 293T whole cell extract with Wnt8a at 30 µg

Lane 12:

Transfected 293T whole cell extract with Wnt8b at 30 µg

Lane 13:

Transfected 293T whole cell extract with Wnt9a at 30 µg

Lane 14:

Transfected 293T whole cell extract with Wnt9b at 30 µg

Lane 15:

Transfected 293T whole cell extract with Wnt10a at 30 µg

Lane 16:

Transfected 293T whole cell extract with Wnt10b at 30 µg

Lane 17:

Transfected 293T whole cell extract with Wnt16 at 30 µg

Lane 18:

Transfected 293T whole cell extract with Wnt11 at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1630

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Synthetic Fragment Protein within Human WNT11. The exact immunogen used to generate this antibody is proprietary information.

O96014

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.

Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

See full target information WNT11

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt11 antibody [HL1630]

Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)

Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)

Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise