Rabbit Monoclonal WNT11 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line, Recombinant fragment samples. Immunogen corresponding to Synthetic Fragment Protein within Human WNT11.
Images
![Western blot - Anti-Wnt11 antibody [HL1630] (AB308177), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-hl1630-ab308177--western-blot-img429485.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)")
![Western blot - Anti-Wnt11 antibody [HL1630] (AB308177), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-hl1630-ab308177--western-blot-img407093.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)")
![Western blot - Anti-Wnt11 antibody [HL1630] (AB308177), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-hl1630-ab308177--western-blot-img407095.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Wnt11 antibody [HL1630] (AB308177)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Fragment Protein within Human WNT11. The exact immunogen used to generate this antibody is proprietary information. Database link O96014
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Recombinant fragment | Tested |
| Transfected cell line | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Transfected cell line | Dilution info 1/1000 - 1/5000 | Notes - |
Species Recombinant fragment | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Alternative names
Protein Wnt-11, WNT11
Recommended products
Rabbit Monoclonal WNT11 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line, Recombinant fragment samples. Immunogen corresponding to Synthetic Fragment Protein within Human WNT11.
Alternative names
Protein Wnt-11, WNT11
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Fragment Protein within Human WNT11. The exact immunogen used to generate this antibody is proprietary information. Database link O96014
- Carrier free
Yes
- Clone number
HL1630
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- Activity summary
Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.
- Biological function summary
The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.
- Pathways
Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.
- Associated diseases and disorders
Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Wnt11 antibody [HL1630] (ab308177), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-hl1630-ab308177--western-blot-img429485.jpg "Western blot - Anti-Wnt11 antibody [HL1630] (ab308177)")
Western blot - Anti-Wnt11 antibody [HL1630] (ab308177)
All lanes: Western blot - Anti-Wnt11 antibody [HL1630] (ab308177) at 1/5000 dilution
Lane 1: Non-transfected 293T whole cell extract at 30 µg
Lane 2: Transfected 293T whole cell extract with Wnt1 at 30 µg
Lane 3: Transfected 293T whole cell extract with Wnt2 at 30 µg
Lane 4: Transfected 293T whole cell extract with Wnt3 at 30 µg
Lane 5: Transfected 293T whole cell extract with Wnt3a at 30 µg
Lane 6: Transfected 293T whole cell extract with Wnt4 at 30 µg
Lane 7: Transfected 293T whole cell extract with Wnt5a at 30 µg
Lane 8: Transfected 293T whole cell extract with Wnt6 at 30 µg
Lane 9: Transfected 293T whole cell extract with Wnt7a at 30 µg
Lane 10: Transfected 293T whole cell extract with Wnt7b at 30 µg
Lane 11: Transfected 293T whole cell extract with Wnt8a at 30 µg
Lane 12: Transfected 293T whole cell extract with Wnt8b at 30 µg
Lane 13: Transfected 293T whole cell extract with Wnt9a at 30 µg
Lane 14: Transfected 293T whole cell extract with Wnt9b at 30 µg
Lane 15: Transfected 293T whole cell extract with Wnt10a at 30 µg
Lane 16: Transfected 293T whole cell extract with Wnt10b at 30 µg
Lane 17: Transfected 293T whole cell extract with Wnt16 at 30 µg
Lane 18: Transfected 293T whole cell extract with Wnt11 at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
![Western blot - Anti-Wnt11 antibody [HL1630] (ab308177), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-hl1630-ab308177--western-blot-img407093.jpg "Western blot - Anti-Wnt11 antibody [HL1630] (ab308177)")
Western blot - Anti-Wnt11 antibody [HL1630] (ab308177)
Separated by 10% SDS-PAGE.
All lanes: Western blot - Anti-Wnt11 antibody [HL1630] (ab308177) at 1/1000 dilution
All lanes: Caco-2 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39.2 kDa
![Western blot - Anti-Wnt11 antibody [HL1630] (ab308177), expandable thumbnail](https://content.abcam.com/products/images/wnt11-antibody-hl1630-ab308177--western-blot-img407095.jpg "Western blot - Anti-Wnt11 antibody [HL1630] (ab308177)")
Western blot - Anti-Wnt11 antibody [HL1630] (ab308177)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody [HL1630] (ab308177) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Wnt11 antibody [HL1630] (ab308177) at 1/5000 dilution
Lane 1: Non-transfected (–) HEK-293T whole cell lysate at 30 µg
Lane 2: Transfected (+) 293T whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39.2 kDa
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