AB189033

Anti-Wnt16 antibody

Name: Anti-Wnt16 antibody (ab189033) | Abcam
Brand: Abcam Limited
SKU: AB189033
Price: 630 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal WNT16 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-16.

View Alternative Names

Protein Wnt-16, WNT16

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt16 antibody (AB189033)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt16 antibody (AB189033)

Immunohistochemical analysis of formalin fixed, paraffin embedded human spleen tissue labeling Wnt16 using ab189033 at 5 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Protein Wnt-16. The exact immunogen used to generate this antibody is proprietary information.

Q9UBV4

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt16 also known as wingless-type MMTV integration site family member 16 is a protein with a mass of approximately 43 kDa. It belongs to the Wnt protein family which comprises secreted signaling proteins. Wnt16 facilitates the transfer of signals between cells influencing various cellular processes. This protein shows expression in several tissues such as bone cartilage and hematopoietic tissues playing roles in maintaining their functions.

Biological function summary

Wnt16 influences bone development and maintenance by enhancing osteoblast proliferation and differentiation. It functions as part of a complex signaling cascade interacting with receptors on cell surfaces to initiate intracellular signaling pathways. One of its primary roles is in balancing bone formation and resorption ensuring skeletal integrity. Furthermore Wnt16 contributes to cartilage homeostasis preventing degenerative changes that could lead to diseases like osteoarthritis.

Pathways

Wnt16 is part of significant signaling pathways like the canonical Wnt/β-catenin pathway as well as the non-canonical Wnt pathways. In the canonical pathway Wnt16 binds to Frizzled receptors and LRP5/6 co-receptors facilitating the stabilization and translocation of β-catenin into the nucleus affecting gene transcription. In this context Wnt16 relates closely to other Wnt proteins like Wnt3a influencing diverse cellular outcomes such as proliferation and differentiation.

Wnt16 has an impact on conditions like osteoporosis and osteoarthritis. In osteoporosis the reduction in Wnt16 expression correlates with decreased bone density and increased risk of fractures. Through its interaction with bone-forming proteins like Osteocalcin Wnt16 regulates bone mass and strength. Additionally in osteoarthritis Wnt16 downregulation leads to increased cartilage degradation linking it to proteins like MMP-13 which is involved in cartilage breakdown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).

See full target information Protein Wnt-16

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 19:e13162 PubMed32567210

2020

Attenuation of ataxia telangiectasia mutated signalling mitigates age-associated intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Han,Chao-Ming Zhou,Hongxing Shen,Jun Tan,Qing Dong,Lei Zhang,Sara J McGowan,Jing Zhao,Gwendolyn A Sowa,James D Kang,Laura J Niedernhofer,Paul D Robbins,Nam N Vo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt16 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt16 antibody (AB189033)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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