Anti-Wnt16 antibody [HL1498] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt16 antibody [HL1498] - BSA and Azide free (AB313859)

Immunofluorescent analysis of mock and Wnt16 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Wnt16 with ab313859 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-Wnt16 antibody [HL1498] - BSA and Azide free (AB313859)

All lanes:

Western blot - Anti-Wnt16 antibody [HL1498] - BSA and Azide free (ab313859) at 1/5000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 30 µg

Lane 2:

Wnt 1 transfected HEK293T whole cell extract at 30 µg

Lane 3:

Wnt 2 transfected HEK293T whole cell extract at 30 µg

Lane 4:

Wnt 2b transfected HEK293T whole cell extract at 30 µg

Lane 5:

Wnt 3 transfected HEK293T whole cell extract at 30 µg

Lane 6:

Wnt 3a transfected HEK293T whole cell extract at 30 µg

Lane 7:

Wnt 4 transfected HEK293T whole cell extract at 30 µg

Lane 8:

Wnt 5a transfected HEK293T whole cell extract at 30 µg

Lane 9:

Wnt 6 transfected HEK293T whole cell extract at 30 µg

Lane 10:

Wnt 7a transfected HEK293T whole cell extract at 30 µg

Lane 11:

Wnt 7b transfected HEK293T whole cell extract at 30 µg

Lane 12:

Wnt 8a transfected HEK293T whole cell extract at 30 µg

Lane 13:

Wnt 8b transfected HEK293T whole cell extract at 30 µg

Lane 14:

Wnt 9a transfected HEK293T whole cell extract at 30 µg

Lane 15:

Wnt 9b transfected HEK293T whole cell extract at 30 µg

Lane 16:

Wnt 10a transfected HEK293T whole cell extract at 30 µg

Lane 17:

Wnt 10b transfected HEK293T whole cell extract at 30 µg

Lane 18:

Wnt 16 transfected HEK293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated secondary

false