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AB227859

Anti-Wnt2/IRP antibody

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(2 Publications)

Rabbit Polyclonal Wnt2/IRP antibody. Suitable for ELISA, WB and reacts with Synthetic peptide, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT2.

View Alternative Names

INT1L1, IRP, WNT2, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein

2 Images
Western blot - Anti-Wnt2/IRP antibody (AB227859)
  • WB

Supplier Data

Western blot - Anti-Wnt2/IRP antibody (AB227859)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Wnt2/IRP antibody (ab227859) at 1/500 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 40 kDa

false

ELISA - Anti-Wnt2/IRP antibody (AB227859)
  • ELISA

Lab

ELISA - Anti-Wnt2/IRP antibody (AB227859)

Analysis of ab227859 showing binding of Wnt2 in ELISA assay

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human WNT2. The exact immunogen used to generate this antibody is proprietary information.

P09544

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Alternative Products

Primary Antibodies

AB109222

Anti-Wnt2/IRP antibody [EPR3101(2)]

RabMAb|Recombinant

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

  • 0
  • 0
Primary Antibodies

AB150608

Anti-Wnt2/IRP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt2/IRP antibody (AB150608)

  • 0
  • 0
Primary Antibodies

AB232036

Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (AB232036)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB219412

Anti-Wnt3a antibody [EPR21889]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412)

  • 0
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Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB15251

Anti-Wnt1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (AB15251)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt2 also known as Wingless-type MMTV integration site family member 2 or IRP is a protein around 41 kDa in mass. It is mechanically involved in signaling processes playing an important role in cell-to-cell communication. Wnt2 is expressed in various tissues including the heart lung and spleen and it participates in embryonic development and organogenesis. This protein belongs to the Wnt family which is known for modulating developmental processes and signaling pathways.
Biological function summary

With respect to the Wnt/β-catenin signaling pathway Wnt2 operates as a ligand that binds to Frizzled receptors on the cell surface. This interaction leads to the stabilization of β-catenin in the cytoplasm impacting gene transcription. Wnt2 does not function alone; it forms complexes with other proteins to efficiently propagate signals that regulate cell fate decisions. It is important in maintaining homeostasis and regeneration processes in adults.

Pathways

Within the Wnt/β-catenin pathway Wnt2 activation regulates important genes involved in differentiation and proliferation. This protein interacts with key molecules like Axin and GSK-3β facilitating the stabilization of β-catenin and subsequent gene transcription. Another pathway involving Wnt2 is the planar cell polarity pathway where it influences cytoskeletal arrangements and directional cell movement through its interaction with Dishevelled proteins.

Research links Wnt2 to cancer progression and fibrotic diseases. Aberrant Wnt2 signaling promotes tumorigenesis through deregulated cell proliferation and survival particularly in gastric and colorectal cancers. In fibrosis overactive Wnt2 signaling contributes to excessive extracellular matrix production with Transforming Growth Factor-β (TGF-β) often collaborating in these processes to exacerbate disease progression. Understanding how Wnt2 interacts with these proteins offers insight into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20018874). Functions as a upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).
See full target information WNT2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0298295 PubMed38354174

2024

SP3-induced Timeless transcription contributes to cell growth of lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Tian,Dajun Du,Li Yang,Nan Zhou,Ling Tao

American journal of cancer research 12:5552-5563 PubMed36628278

2022

Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxiu Xu,Mingjie Chen,Mingyu Hu,Hanlu Wang,Zhongqiang Zuo,Jianguo Wang,Zhiyin Xie
View all publications
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Product promise

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