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AB109222

Anti-Wnt2/IRP antibody [EPR3101(2)]

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(15 Publications)

Rabbit Recombinant Monoclonal Wnt2/IRP antibody. Suitable for WB, I-ELISA and reacts with Mouse, Rat, Human, Synthetic peptide - Human samples. Cited in 15 publications.

View Alternative Names

INT1L1, IRP, WNT2, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein

5 Images
Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)
  • WB

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Representative standard curve using ab109222

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)
  • WB

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

SKBR-3 cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)
  • WB

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)
  • WB

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

MCF-7 cell lysates at 10 µg

Lane 3:

T47-D cell lysates at 10 µg

Lane 4:

SKBR-3 cell lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

  • Carrier free

    Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3101(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt2 also known as Wingless-type MMTV integration site family member 2 or IRP is a protein around 41 kDa in mass. It is mechanically involved in signaling processes playing an important role in cell-to-cell communication. Wnt2 is expressed in various tissues including the heart lung and spleen and it participates in embryonic development and organogenesis. This protein belongs to the Wnt family which is known for modulating developmental processes and signaling pathways.
Biological function summary

With respect to the Wnt/β-catenin signaling pathway Wnt2 operates as a ligand that binds to Frizzled receptors on the cell surface. This interaction leads to the stabilization of β-catenin in the cytoplasm impacting gene transcription. Wnt2 does not function alone; it forms complexes with other proteins to efficiently propagate signals that regulate cell fate decisions. It is important in maintaining homeostasis and regeneration processes in adults.

Pathways

Within the Wnt/β-catenin pathway Wnt2 activation regulates important genes involved in differentiation and proliferation. This protein interacts with key molecules like Axin and GSK-3β facilitating the stabilization of β-catenin and subsequent gene transcription. Another pathway involving Wnt2 is the planar cell polarity pathway where it influences cytoskeletal arrangements and directional cell movement through its interaction with Dishevelled proteins.

Research links Wnt2 to cancer progression and fibrotic diseases. Aberrant Wnt2 signaling promotes tumorigenesis through deregulated cell proliferation and survival particularly in gastric and colorectal cancers. In fibrosis overactive Wnt2 signaling contributes to excessive extracellular matrix production with Transforming Growth Factor-β (TGF-β) often collaborating in these processes to exacerbate disease progression. Understanding how Wnt2 interacts with these proteins offers insight into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20018874). Functions as a upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).
See full target information WNT2
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202976 PubMed36529961

2022

Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Shiji Deng,Shu Shu,Lili Zhai,Shengnan Xia,Xiang Cao,Huiya Li,Xinyu Bao,Pinyi Liu,Yun Xu

Journal of Cancer 12:4196-4208 PubMed34093820

2021

MicroRNA-1246 by Targeting AXIN2 and GSK-3β Overcomes Drug Resistance and Induces Apoptosis in Chemo-resistant Leukemia Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Xie,Linjing Li,Zhewen Zhang,Lei Zhao,Juan Cheng,Cunmin Zhou,Jie Cheng,Jing Yan,Jing Chen,Juan Yi,Bei Wang,Suya Jin,Hulai Wei

Journal of cellular and molecular medicine 25:4696-4708 PubMed33787057

2021

SNHG12 promotes carcinogenesis of human renal cell cancer via functioning as a competing endogenous RNA and sponging miR-30a-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyuan Yu,Junlong Liu,Zhe Zhang,Yuyan Zhu,Jianbin Bi,Chuize Kong

BMC molecular and cell biology 21:80 PubMed33167868

2020

Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Chen,Jiuyang Jiao,Youyuan Wang,Zhihui Mai,Jing Ren,Sijie He,Xiaolan Li,Zheng Chen

Molecular medicine reports 22:5053-5068 PubMed33174043

2020

Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Guofang Guan,Xiao He,Jingjing Chen,Li Bin,Xuxia Tang

Journal of biochemical and molecular toxicology 34:e22454 PubMed31981282

2020

ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR-8/SVneo cells via modulating the epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohan Zou,Ruirui Dong,Ping Zou,Xina Meng,Ting Zhang,Liang Luo,Na Li,Yao Wang,Jing Wang,Tiejun Wang,Yan Zhang,Minjian Chen,Conghua Zhou,Fei Han

JCI insight 4: PubMed31647783

2019

Sonic hedgehog connects podocyte injury to mesangial activation and glomerulosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhou,Haiyan Fu,Yang Han,Lu Zhang,Shijia Liu,Lin Lin,Donna B Stolz,Youhua Liu

Molecular medicine reports 20:1808-1818 PubMed31257529

2019

Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β‑catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Xie,Lei Zhao,Lanlan Guo,Hang Liu,Siyu Fu,Wenjuan Fan,Li Lin,Jing Chen,Bei Wang,Linlan Fan,Hulai Wei

Pancreatology : official journal of the Internatio 19:252-257 PubMed30792046

2019

Wnt/β-catenin signalling plays diverse functions during the process of fibrotic remodelling in the exocrine pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Merja Bläuer,Matias Laaninen,Juhani Sand,Johanna Laukkarinen

Oncology letters 16:2857-2864 PubMed30127872

2018

Wnt2 contributes to the progression of gastric cancer by promoting cell migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoran Zhang,Jinyan Wang,Xiang Dong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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