AB109222

Anti-Wnt2/IRP antibody [EPR3101(2)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(20 Publications)

Rabbit Recombinant Monoclonal Wnt2/IRP antibody. Suitable for WB, I-ELISA and reacts with Mouse, Rat, Human, Synthetic peptide - Human samples. Cited in 20 publications.

View Alternative Names

INT1L1, IRP, WNT2, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein

5 Images

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

SKBR-3 cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Representative standard curve using ab109222

Unknown

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

MCF-7 cell lysates at 10 µg

Lane 3:

T47-D cell lysates at 10 µg

Lane 4:

SKBR-3 cell lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 34 kDa

false

Carrier free

Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3101(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt2 also known as Wingless-type MMTV integration site family member 2 or IRP is a protein around 41 kDa in mass. It is mechanically involved in signaling processes playing an important role in cell-to-cell communication. Wnt2 is expressed in various tissues including the heart lung and spleen and it participates in embryonic development and organogenesis. This protein belongs to the Wnt family which is known for modulating developmental processes and signaling pathways.

Biological function summary

With respect to the Wnt/β-catenin signaling pathway Wnt2 operates as a ligand that binds to Frizzled receptors on the cell surface. This interaction leads to the stabilization of β-catenin in the cytoplasm impacting gene transcription. Wnt2 does not function alone; it forms complexes with other proteins to efficiently propagate signals that regulate cell fate decisions. It is important in maintaining homeostasis and regeneration processes in adults.

Pathways

Within the Wnt/β-catenin pathway Wnt2 activation regulates important genes involved in differentiation and proliferation. This protein interacts with key molecules like Axin and GSK-3β facilitating the stabilization of β-catenin and subsequent gene transcription. Another pathway involving Wnt2 is the planar cell polarity pathway where it influences cytoskeletal arrangements and directional cell movement through its interaction with Dishevelled proteins.

Research links Wnt2 to cancer progression and fibrotic diseases. Aberrant Wnt2 signaling promotes tumorigenesis through deregulated cell proliferation and survival particularly in gastric and colorectal cancers. In fibrosis overactive Wnt2 signaling contributes to excessive extracellular matrix production with Transforming Growth Factor-β (TGF-β) often collaborating in these processes to exacerbate disease progression. Understanding how Wnt2 interacts with these proteins offers insight into potential therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20018874). Functions as a upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).

See full target information WNT2

style

left-column

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 166: PubMed39951495

2025

Tenascin-C Potentiates Wnt Signaling in Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Heather A Hartmann,Matthew A Loberg,George J Xu,Anna C Schwarzkopf,Sheau-Chiann Chen,Courtney J Phifer,Kailey Caroland,Hua-Chang Chen,Diana Diaz,Megan L Tigue,Amanda B Hesterberg,Jean-Nicolas Gallant,Sophia M Shaddy,Quanhu Sheng,James L Netterville,Sarah L Rohde,Carmen C Solórzano,Lindsay A Bischoff,Naira Baregamian,Paula J Hurley,Barbara A Murphy,Jennifer H Choe,Eric C Huang,Fei Ye,Ethan Lee,Vivian L Weiss

American journal of cancer research 13:2530-2539 PubMed37424817

2023

Claudin 4 enhances the malignancy of glioma cells via NNAT/Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Feilong Yang,Wuhuan Xu,Xielin Tang,Qianke Li,Xiaolin Hou,Xuhui Hui

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22776 PubMed36688817

2023

Loss of aquaporin 5 contributes to the corneal epithelial pathogenesis via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Guohu Di,Kaier Zhang,Ying Bai,Xin Cao,Hui Zhao,Dianqiang Wang,Peng Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202976 PubMed36529961

2022

Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Shiji Deng,Shu Shu,Lili Zhai,Shengnan Xia,Xiang Cao,Huiya Li,Xinyu Bao,Pinyi Liu,Yun Xu

Environmental toxicology 37:910-924 PubMed34995009

2022

Inhibition of ALKBH5-mediated m A modification of PPARG mRNA alleviates H/R-induced oxidative stress and apoptosis in placenta trophoblast.

Applications

Unspecified application

Species

Unspecified reactive species

Yongping Guo,Wenxia Song,Yali Yang

Journal of Cancer 12:4196-4208 PubMed34093820

2021

MicroRNA-1246 by Targeting AXIN2 and GSK-3β Overcomes Drug Resistance and Induces Apoptosis in Chemo-resistant Leukemia Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Xie,Linjing Li,Zhewen Zhang,Lei Zhao,Juan Cheng,Cunmin Zhou,Jie Cheng,Jing Yan,Jing Chen,Juan Yi,Bei Wang,Suya Jin,Hulai Wei

Journal of cellular and molecular medicine 25:4696-4708 PubMed33787057

2021

SNHG12 promotes carcinogenesis of human renal cell cancer via functioning as a competing endogenous RNA and sponging miR-30a-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyuan Yu,Junlong Liu,Zhe Zhang,Yuyan Zhu,Jianbin Bi,Chuize Kong

BMC molecular and cell biology 21:80 PubMed33167868

2020

Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Chen,Jiuyang Jiao,Youyuan Wang,Zhihui Mai,Jing Ren,Sijie He,Xiaolan Li,Zheng Chen

Molecular medicine reports 22:5053-5068 PubMed33174043

2020

Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Guofang Guan,Xiao He,Jingjing Chen,Li Bin,Xuxia Tang

Journal of biochemical and molecular toxicology 34:e22454 PubMed31981282

2020

ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR-8/SVneo cells via modulating the epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohan Zou,Ruirui Dong,Ping Zou,Xina Meng,Ting Zhang,Liang Luo,Na Li,Yao Wang,Jing Wang,Tiejun Wang,Yan Zhang,Minjian Chen,Conghua Zhou,Fei Han

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt2/IRP antibody [EPR3101(2)]

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise