AB232036

Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Wnt2/IRP antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

INT1L1, IRP, WNT2, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein

2 Images

Supplier Data

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (AB232036)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109222).

All lanes:

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (ab232036) at 1/5000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 40 kDa

false

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (AB232036)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (AB232036)

This data was developed using ab109222, the same antibody clone in a different buffer formulation.

Representative standard curve using ab109222

Unconjugated

Anti-Wnt2/IRP antibody [EPR3101(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3101(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Can be blocked with <a href='/en-us/products/proteins-peptides/wnt2-irp-peptide-ab197186'>ab197186</a>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Can be blocked with <a href='/en-us/products/proteins-peptides/wnt2-irp-peptide-ab197186'>ab197186</a>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Can be blocked with <a href='/en-us/products/proteins-peptides/wnt2-irp-peptide-ab197186'>ab197186</a>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Product details

ab232036 is the carrier-free version of ab109222.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt2 also known as Wingless-type MMTV integration site family member 2 or IRP is a protein around 41 kDa in mass. It is mechanically involved in signaling processes playing an important role in cell-to-cell communication. Wnt2 is expressed in various tissues including the heart lung and spleen and it participates in embryonic development and organogenesis. This protein belongs to the Wnt family which is known for modulating developmental processes and signaling pathways.

Biological function summary

With respect to the Wnt/β-catenin signaling pathway Wnt2 operates as a ligand that binds to Frizzled receptors on the cell surface. This interaction leads to the stabilization of β-catenin in the cytoplasm impacting gene transcription. Wnt2 does not function alone; it forms complexes with other proteins to efficiently propagate signals that regulate cell fate decisions. It is important in maintaining homeostasis and regeneration processes in adults.

Pathways

Within the Wnt/β-catenin pathway Wnt2 activation regulates important genes involved in differentiation and proliferation. This protein interacts with key molecules like Axin and GSK-3β facilitating the stabilization of β-catenin and subsequent gene transcription. Another pathway involving Wnt2 is the planar cell polarity pathway where it influences cytoskeletal arrangements and directional cell movement through its interaction with Dishevelled proteins.

Research links Wnt2 to cancer progression and fibrotic diseases. Aberrant Wnt2 signaling promotes tumorigenesis through deregulated cell proliferation and survival particularly in gastric and colorectal cancers. In fibrosis overactive Wnt2 signaling contributes to excessive extracellular matrix production with Transforming Growth Factor-β (TGF-β) often collaborating in these processes to exacerbate disease progression. Understanding how Wnt2 interacts with these proteins offers insight into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20018874). Functions as a upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).

See full target information WNT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 10:2523-2538 PubMed34295659

2021

One carbon metabolism in human lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Yao,Luogen Peng,Omar Elakad,Stefan Küffer,Marc Hinterthaner,Bernhard C Danner,Alexander von Hammerstein-Equord,Philipp Ströbel,Hanibal Bohnenberger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (AB232036)

Indirect ELISA - Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (AB232036)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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