JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB203225

Anti-Wnt2b antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal WNT2B antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human WNT2B aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

WNT13, WNT2B, Protein Wnt-2b, Protein Wnt-13

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt2b antibody (AB203225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt2b antibody (AB203225)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Wnt2b using ab203225 at 1/250, followed by secondary detection and DAB staining.

Western blot - Anti-Wnt2b antibody (AB203225)
  • WB

Supplier Data

Western blot - Anti-Wnt2b antibody (AB203225)

All lanes:

Western blot - Anti-Wnt2b antibody (ab203225) at 1/200 dilution

Lane 1:

rat brain lysate

Lane 2:

mouse intestine lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human WNT2B aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q93097

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt2b also known as Wingless-type MMTV integration site family member 2B plays a mechanical role as a ligand in the Wnt signaling pathway. The protein weighs around 41 kDa and is expressed in various tissues including the brain lung and reproductive organs. As a signaling molecule Wnt2b binds to receptor complexes on cell surfaces initiating intracellular signaling cascades that regulate cellular processes.
Biological function summary

The Wnt2b protein influences embryonic development and cell fate decisions. It engages in cellular proliferation differentiation and migration. Wnt2b is a component of the Wnt signaling protein family known for forming signaling complexes with Frizzled receptors. Its role in establishing cell polarity and maintaining stem cell pluripotency makes it important for tissue development and repair.

Pathways

Wnt2b contributes to Wnt/β-catenin and non-canonical Wnt signaling pathways. Wnt/β-catenin signaling is vital for gene transcription regulation where Wnt2b partners with proteins like β-catenin and Axin to modulate these pathways. Non-canonical Wnt signaling impacts cellular architecture and movement without involving β-catenin linking Wnt2b to other proteins through alternative signaling routes.

Wnt2b-related disruptions often tie to cancer and bone density disorders. Aberrant Wnt2b expression connects to cancers such as colorectal cancer linking it with other related Wnt family members and tumor suppressor proteins like APC. Alterations in Wnt2b's function may also associate with bone density disorders by affecting osteoblast differentiation with implications for conditions like osteoporosis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
See full target information WNT2B
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:162 PubMed36849544

2023

Dysfunctional mitochondria accumulate in a skeletal muscle knockout model of Smn1, the causal gene of spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Chemello,Michela Pozzobon,Lorenza Iolanda Tsansizi,Tatiana Varanita,Rubèn Quintana-Cabrera,Daniele Bonesso,Martina Piccoli,Gerolamo Lanfranchi,Marta Giacomello,Luca Scorrano,Camilla Bean

Stem cell reviews and reports 18:2059-2073 PubMed35471485

2022

Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/β-catenin Signaling Pathway in Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Li,Yu An,Yu Sun,Fan Yang,Quanchen Xu,Zhiguo Wang

Diabetology & metabolic syndrome 14:33 PubMed35193668

2022

LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Wei Wang,Weizhong HuangFu,Zhonghua Liu,Feng Zhao

Frontiers in cell and developmental biology 8:528155 PubMed33195183

2020

Hsa_circ_0088233 Alleviates Proliferation, Migration, and Invasion of Prostate Cancer by Targeting hsa-miR-185-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hai Deng,Gan-Shen Yu,Ke-Lei Deng,Zhen-Hua Feng,Qiang Huang,Bin Pan,Jian-Zhong Deng

Reproductive toxicology (Elmsford, N.Y.) 91:59-73 PubMed31705956

2019

Abnormal retinal pigment epithelium melanogenesis as a major determinant for radiation-induced congenital eye defects.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Craenen,Mieke Verslegers,Livine Craeghs,Roel Quintens,Ann Janssen,Amelie Coolkens,Sarah Baatout,Lieve Moons,Mohammed Abderrafi Benotmane
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109222

Anti-Wnt2/IRP antibody [EPR3101(2)]

RabMAb|Recombinant

Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

  • 0
  • 0
Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

  • 5
  • 75
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com