Rabbit Polyclonal WNT2B antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human WNT2B aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Stem cell reviews and reports 18:2059-20732022Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/β-catenin Signaling Pathway in Dermal Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Li,Yu An,Yu Sun,Fan Yang,Quanchen Xu,Zhiguo WangPubMed 35471485
- Diabetology & metabolic syndrome 14:332022LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wang,Wei Wang,Weizhong HuangFu,Zhonghua Liu,Feng ZhaoPubMed 35193668
- Frontiers in cell and developmental biology 8:5281552020Hsa_circ_0088233 Alleviates Proliferation, Migration, and Invasion of Prostate Cancer by Targeting hsa-miR-185-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Hai Deng et. al.PubMed 33195183
- Reproductive toxicology (Elmsford, N.Y.) 91:59-732019Abnormal retinal pigment epithelium melanogenesis as a major determinant for radiation-induced congenital eye defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Craenen,Mieke Verslegers,Livine Craeghs,Roel Quintens,Ann Janssen,Amelie Coolkens,Sarah Baatout,Lieve Moons,Mohammed Abderrafi BenotmanePubMed 31705956
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WNT2B aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q93097
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
Alternative names
WNT13, WNT2B, Protein Wnt-2b, Protein Wnt-13
Recommended products
Rabbit Polyclonal WNT2B antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human WNT2B aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human WNT2B aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q93097
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt2b also known as Wingless-type MMTV integration site family member 2B plays a mechanical role as a ligand in the Wnt signaling pathway. The protein weighs around 41 kDa and is expressed in various tissues including the brain lung and reproductive organs. As a signaling molecule Wnt2b binds to receptor complexes on cell surfaces initiating intracellular signaling cascades that regulate cellular processes.
- Biological function summary
The Wnt2b protein influences embryonic development and cell fate decisions. It engages in cellular proliferation differentiation and migration. Wnt2b is a component of the Wnt signaling protein family known for forming signaling complexes with Frizzled receptors. Its role in establishing cell polarity and maintaining stem cell pluripotency makes it important for tissue development and repair.
- Pathways
Wnt2b contributes to Wnt/β-catenin and non-canonical Wnt signaling pathways. Wnt/β-catenin signaling is vital for gene transcription regulation where Wnt2b partners with proteins like β-catenin and Axin to modulate these pathways. Non-canonical Wnt signaling impacts cellular architecture and movement without involving β-catenin linking Wnt2b to other proteins through alternative signaling routes.
- Associated diseases and disorders
Wnt2b-related disruptions often tie to cancer and bone density disorders. Aberrant Wnt2b expression connects to cancers such as colorectal cancer linking it with other related Wnt family members and tumor suppressor proteins like APC. Alterations in Wnt2b's function may also associate with bone density disorders by affecting osteoblast differentiation with implications for conditions like osteoporosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt2b antibody (ab203225)
Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Wnt2b using ab203225 at 1/250, followed by secondary detection and DAB staining.
Western blot - Anti-Wnt2b antibody (ab203225)
All lanes: Western blot - Anti-Wnt2b antibody (ab203225) at 1/200 dilution
Lane 1: rat brain lysate
Lane 2: mouse intestine lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 44 kDa
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