AB116222

Anti-Wnt3 antibody

Name: Anti-Wnt3 antibody (ab116222) | Abcam
Brand: Abcam Limited
SKU: AB116222
Price: 645 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(8 Publications)

Goat Polyclonal WNT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human WNT3 aa 300-350.

View Alternative Names

INT4, WNT3, Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-Wnt3 antibody (AB116222)

All lanes:

Western blot - Anti-Wnt3 antibody (ab116222) at 1 µg/mL

All lanes:

Human brain lysate (in RIPA buffer) at 35 µg

Predicted band size: 40 kDa

true

Exposure time: 1hr

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human WNT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P56703

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt3 also known as Wingless-related integration site 3 or Proto-oncogene Wnt-3 plays an important mechanical role in cellular signaling. It is a protein with a mass of approximately 38 kDa. Wnt3 is expressed richly across various tissues including brain heart and skeletal muscle. This protein is instrumental in initiating signaling cascades that influence cell behavior during development.

Biological function summary

Wnt3 functions in the Wnt signaling pathway to regulate gene transcription cellular polarity and cell fate determinations. As a member of the Wnt protein family Wnt3 contributes to a protein complex on the cell surface interacting with cell surface receptors such as frizzled receptors. These interactions trigger intracellular signaling pathways that promote developmental processes and maintain cellular communication networks.

Pathways

Wnt3 is integral to the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. In the canonical pathway interactions with β-catenin lead to gene expression changes critical for cell differentiation and proliferation. In the non-canonical pathway Wnt3 associates with proteins like Dishevelled and phosphoproteins to influence cytoskeletal dynamics and cellular movement. These pathways highlight Wnt3's involvement in critical cellular functions and developmental processes.

Aberrant Wnt3 signaling is linked to various conditions including cancer and osteoporosis. The protein's role in cancer often together with the β-catenin protein reveals connections in tumor growth and metastasis. In osteoporosis dysregulation of Wnt3 impacts bone density and strength as it affects osteoblast function in bone formation. These associations highlight Wnt3's significant involvement in pathological states when its signaling is disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed : 14872406).

See full target information WNT3

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics 19:2369006 PubMed38913848

2024

YY1/circCTNNB1/miR-186-5p/YY1 positive loop aggravates lung cancer progression through the Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Shen,Yan Yang,Yan Zhao,Saiteer Nuerlan,Yiyi Zhan,Chunling Liu

Pharmaceuticals (Basel, Switzerland) 17: PubMed38256932

2024

Liuwei Dihuang Pills Enhance Osteogenic Differentiation in MC3T3-E1 Cells through the Activation of the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Zhao,Guihong Liang,Junzheng Yang,Hetao Huang,Yaoxing Dou,Zhuoxu Gu,Jun Liu,Lingfeng Zeng,Weiyi Yang

Aging 15:11033-11051 PubMed37837551

2023

Dysregulated expression of slingshot protein phosphatase 1 (SSH1) disrupts circadian rhythm and WNT signaling associated to hepatocellular carcinoma pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shiue-Wei Lai,Yi-Chiao Cheng,Wen-Chien Huang,Vijesh Kumar Yadav,Iat-Hang Fong,Chi-Tai Yeh,Ching-Kuo Yang,Wei-Hwa Lee,Ming-Yao Chen

Cellular and molecular life sciences : CMLS 78:6995-7008 PubMed34608506

2021

WNT3 hypomethylation counteracts low activity of the Wnt signaling pathway in the placenta of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhang,Min Sang,Ying Li,Yingying Li,Erfeng Yuan,Lijun Yang,Wenli Shi,Yangyang Yuan,Bo Yang,Peifeng Yang,Enwu Yuan

Poultry science 100:101283 PubMed34229217

2021

Lactobacillus casei protects intestinal mucosa from damage in chicks caused by Salmonella pullorum via regulating immunity and the Wnt signaling pathway and maintaining the abundance of gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Ziteng Deng,Deping Han,Yuying Wang,Qiuzhen Wang,Xue Yan,Shujing Wang,Xuelian Liu,Weiping Song,Yunfei Ma

Artificial cells, nanomedicine, and biotechnology 48:326-335 PubMed31878795

2019

Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer and .

Applications

Unspecified application

Species

Unspecified reactive species

Zhijia Yang,Guoqing Li,Chao Ding,Wencong Sun,Ji Zhang

Journal of cellular physiology 235:2963-2972 PubMed31538334

2019

Propofol exerts anticancer activity on hepatocellular carcinoma cells by raising lncRNA DGCR5.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Hai Sun

Molecular human reproduction 21:711-22 PubMed26108805

2015

WNT3 and membrane-associated β-catenin regulate trophectoderm lineage differentiation in human blastocysts.

Applications

Unspecified application

Species

Unspecified reactive species

M Krivega,W Essahib,H Van de Velde

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Wnt3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB116222)

Western blot - Anti-Wnt3 antibody (AB116222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise