Goat Polyclonal WNT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human WNT3 aa 300-350.
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Publications
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- Aging 15:11033-110512023Dysregulated expression of slingshot protein phosphatase 1 (SSH1) disrupts circadian rhythm and WNT signaling associated to hepatocellular carcinoma pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiue-Wei Lai,Yi-Chiao Cheng,Wen-Chien Huang,Vijesh Kumar Yadav,Iat-Hang Fong,Chi-Tai Yeh,Ching-Kuo Yang,Wei-Hwa Lee,Ming-Yao ChenPubMed 37837551
- Cellular and molecular life sciences : CMLS 78:6995-70082021WNT3 hypomethylation counteracts low activity of the Wnt signaling pathway in the placenta of preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlin Zhang,Min Sang,Ying Li,Yingying Li,Erfeng Yuan,Lijun Yang,Wenli Shi,Yangyang Yuan,Bo Yang,Peifeng Yang,Enwu YuanPubMed 34608506
- Poultry science 100:1012832021Lactobacillus casei protects intestinal mucosa from damage in chicks caused by Salmonella pullorum via regulating immunity and the Wnt signaling pathway and maintaining the abundance of gut microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiteng Deng,Deping Han,Yuying Wang,Qiuzhen Wang,Xue Yan,Shujing Wang,Xuelian Liu,Weiping Song,Yunfei MaPubMed 34229217
- Artificial cells, nanomedicine, and biotechnology 48:326-3352019Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijia Yang,Guoqing Li,Chao Ding,Wencong Sun,Ji ZhangPubMed 31878795
- Journal of cellular physiology 235:2963-29722019Propofol exerts anticancer activity on hepatocellular carcinoma cells by raising lncRNA DGCR5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Sun,Hai SunPubMed 31538334
- Molecular human reproduction 21:711-222015WNT3 and membrane-associated β-catenin regulate trophectoderm lineage differentiation in human blastocysts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Krivega et. al.PubMed 26108805
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WNT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P56703
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Dog, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Dog, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed:14872406).
Alternative names
INT4, WNT3, Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog
Recommended products
Goat Polyclonal WNT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human WNT3 aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human WNT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P56703
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt3 also known as Wingless-related integration site 3 or Proto-oncogene Wnt-3 plays an important mechanical role in cellular signaling. It is a protein with a mass of approximately 38 kDa. Wnt3 is expressed richly across various tissues including brain heart and skeletal muscle. This protein is instrumental in initiating signaling cascades that influence cell behavior during development.
- Biological function summary
Wnt3 functions in the Wnt signaling pathway to regulate gene transcription cellular polarity and cell fate determinations. As a member of the Wnt protein family Wnt3 contributes to a protein complex on the cell surface interacting with cell surface receptors such as frizzled receptors. These interactions trigger intracellular signaling pathways that promote developmental processes and maintain cellular communication networks.
- Pathways
Wnt3 is integral to the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. In the canonical pathway interactions with β-catenin lead to gene expression changes critical for cell differentiation and proliferation. In the non-canonical pathway Wnt3 associates with proteins like Dishevelled and phosphoproteins to influence cytoskeletal dynamics and cellular movement. These pathways highlight Wnt3's involvement in critical cellular functions and developmental processes.
- Associated diseases and disorders
Aberrant Wnt3 signaling is linked to various conditions including cancer and osteoporosis. The protein's role in cancer often together with the β-catenin protein reveals connections in tumor growth and metastasis. In osteoporosis dysregulation of Wnt3 impacts bone density and strength as it affects osteoblast function in bone formation. These associations highlight Wnt3's significant involvement in pathological states when its signaling is disrupted.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Wnt3 antibody (ab116222)
All lanes: Western blot - Anti-Wnt3 antibody (ab116222) at 1 µg/mL
All lanes: Human brain lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 1hr
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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