Anti-Wnt3 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (AB50341)

ab50341 (2μg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.

• WB

Unknown

Western blot - Anti-Wnt3 antibody (AB50341)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Wnt3 antibody (ab50341) at 1 µg/mL

All lanes:

Human Brain lysate (35µg protein in RIPA buffer).

Predicted band size: 40 kDa

Observed band size: 17 kDa,38 kDa

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• WB

CiteAb

Western blot - Anti-Wnt3 antibody (AB50341)

Wnt3 western blot using anti-Wnt3 antibody ab50341. Publication image and figure legend from Tripathy, A., Thakurela, S., et al., 2018, PLoS One, PubMed 30513115.

ab50341 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50341 please see the product overview.

The higher activity of Wnt and Hh pathway in promotion-group correlates with moderately-differentiated/grade-II HCC.Enhanced Wnt and Hh signaling correlates with moderately differentiated HCC. (A) H and E stained liver sections of control and promotion group (group2) animals showing moderately differentiated, thick trabecular pattern, followed by photomicraphs showing IHC staining of Wnt and Hh pathway molecules at TE and TL phases of promotion stage. The corresponding IHC staining intensity graph is shown in the side panel of figures.Y-axis represents labeling index (%) visual score in each intensity graph. (B) Increasing mRNA expression level of Wnt and Hh pathway molecules of control and group2 animals at TE and TL phases of promotion stage. (C) The figure represents fold increase in relative mRNA expression, calculated with respect to GAPDH for Shh, Ptch1, Gli1, Wnt3 β-catenin in control and group2 animals at TE and TL phases of promotion stage (D) Analysis of Wnt and Hh pathway molecules in rat tissue lysates at TE and TL phases of promotion stage by ELISA. Data presented are representative of three independent experiments performed in triplicates and expressed as Mean±S.D. ** and *** differs significantly at p < 0.005 and <0.0005.

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