Goat Polyclonal WNT3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human WNT3 aa 300-350.
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Publications
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- Cell host & microbe 24:833-846.e62018Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Soo Lee,Tae-Young Kim,Yeji Kim,Su-Hyun Lee,Seungil Kim,Sung Wan Kang,Jin-Young Yang,In-Jeoung Baek,Young Hoon Sung,Yun-Yong Park,Sung Wook Hwang,Eunju O,Kwang Soon Kim,Siqing Liu,Nobuhiko Kamada,Nan Gao,Mi-Na KweonPubMed 30543778
- PloS one 13:e02081942018The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnindita Tripathy,Sudhir Thakurela,Manoj Kumar Sahu,Kanishka Uthanasingh,Manas Behera,Amrendra Kumar Ajay,Ratna KumariPubMed 30513115
- Cell death and differentiation 26:426-4422018c-Myc inhibits myoblast differentiation and promotes myoblast proliferation and muscle fibre hypertrophy by regulating the expression of its target genes, miRNAs and lincRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Luo,Jiahui Chen,Limin Li,Xueyi Ren,Tian Cheng,Shiyi Lu,Raman Akinyanju Lawal,Qinghua Nie,Xiquan Zhang,Olivier HanottePubMed 29786076
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WNT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P56703
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed:14872406).
Alternative names
INT4, WNT3, Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog
Recommended products
Goat Polyclonal WNT3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human WNT3 aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human WNT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P56703
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt3 also known as Wingless-related integration site 3 or Proto-oncogene Wnt-3 plays an important mechanical role in cellular signaling. It is a protein with a mass of approximately 38 kDa. Wnt3 is expressed richly across various tissues including brain heart and skeletal muscle. This protein is instrumental in initiating signaling cascades that influence cell behavior during development.
- Biological function summary
Wnt3 functions in the Wnt signaling pathway to regulate gene transcription cellular polarity and cell fate determinations. As a member of the Wnt protein family Wnt3 contributes to a protein complex on the cell surface interacting with cell surface receptors such as frizzled receptors. These interactions trigger intracellular signaling pathways that promote developmental processes and maintain cellular communication networks.
- Pathways
Wnt3 is integral to the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. In the canonical pathway interactions with β-catenin lead to gene expression changes critical for cell differentiation and proliferation. In the non-canonical pathway Wnt3 associates with proteins like Dishevelled and phosphoproteins to influence cytoskeletal dynamics and cellular movement. These pathways highlight Wnt3's involvement in critical cellular functions and developmental processes.
- Associated diseases and disorders
Aberrant Wnt3 signaling is linked to various conditions including cancer and osteoporosis. The protein's role in cancer often together with the β-catenin protein reveals connections in tumor growth and metastasis. In osteoporosis dysregulation of Wnt3 impacts bone density and strength as it affects osteoblast function in bone formation. These associations highlight Wnt3's significant involvement in pathological states when its signaling is disrupted.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab50341)
ab50341 (2μg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
Western blot - Anti-Wnt3 antibody (ab50341)
Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-Wnt3 antibody (ab50341) at 1 µg/mL
All lanes: Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 40 kDa
Observed band size: 17 kDa, 38 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Wnt3 antibody (ab50341)
Wnt3 western blot using anti-Wnt3 antibody ab50341. Publication image and figure legend from Tripathy, A., Thakurela, S., et al., 2018, PLoS One, PubMed 30513115.
ab50341 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50341 please see the product overview.
The higher activity of Wnt and Hh pathway in promotion-group correlates with moderately-differentiated/grade-II HCC.Enhanced Wnt and Hh signaling correlates with moderately differentiated HCC. (A) H and E stained liver sections of control and promotion group (group2) animals showing moderately differentiated, thick trabecular pattern, followed by photomicraphs showing IHC staining of Wnt and Hh pathway molecules at TE and TL phases of promotion stage. The corresponding IHC staining intensity graph is shown in the side panel of figures.Y-axis represents labeling index (%) visual score in each intensity graph. (B) Increasing mRNA expression level of Wnt and Hh pathway molecules of control and group2 animals at TE and TL phases of promotion stage. (C) The figure represents fold increase in relative mRNA expression, calculated with respect to GAPDH for Shh, Ptch1, Gli1, Wnt3 β-catenin in control and group2 animals at TE and TL phases of promotion stage (D) Analysis of Wnt and Hh pathway molecules in rat tissue lysates at TE and TL phases of promotion stage by ELISA. Data presented are representative of three independent experiments performed in triplicates and expressed as Mean±S.D. ** and *** differs significantly at p <0.005 and <0.0005.
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