AB81614

Anti-Wnt3a antibody [3A6]

Name: Anti-Wnt3a antibody [3A6] (ab81614) | Abcam
Brand: Abcam Limited
SKU: AB81614
Rating: 3 (2 reviews)

(2 Reviews)

(21 Publications)

Mouse Monoclonal WNT3A antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WNT3A.

View Alternative Names

Protein Wnt-3a, WNT3A

1 Images

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Western blot - Anti-Wnt3a antibody [3A6] (AB81614)

All lanes:

Western blot - Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution

Lane 1:

293T cell lysate at 40 µg

Lane 2:

Wnt3a transfected 293T cell lysate at 40 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A6

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human WNT3A.

P56704

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 17:1989-1997 PubMed41019940

2025

Neuropilin-2 Mediates Radioresistance in Nasopharyngeal Carcinoma via Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Chen,Huimin Fu,Xiaopeng Huang,Zetan Chen

Scientific reports 14:27053 PubMed39511359

2024

Rhomboid-like 2 correlated with TME infiltration inhibits cuproptosis-related genes and drives malignant phenotype in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifei Che,Wenyi Jin,Yaoxi Wu,Haoyong Li,Peiyu Liang

Bioengineered 13:3070-3081 PubMed35100096

2022

High hydrostatic pressure (30 atm) enhances the apoptosis and inhibits the proteoglycan synthesis and extracellular matrix level of human nucleus pulposus cells via promoting the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zongting Shi,Jun He,Jian He,Yuan Xu

Molecular therapy. Nucleic acids 26:1318-1335 PubMed34853730

2021

TGFB3-AS1 promotes Hcy-induced inflammationof macrophages via inhibiting the maturityof miR-144 and upregulating Rap1a.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Yinju Hao,Anning Yang,Lin Xie,Ning Ding,Lingbo Xu,Yanhua Wang,Yong Yang,Yongsheng Bai,Huiping Zhang,Yideng Jiang

Nature communications 12:362 PubMed33441552

2021

Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Lung Wu,Amanda Dicks,Nancy Steward,Ruhang Tang,Dakota B Katz,Yun-Rak Choi,Farshid Guilak

Frontiers in cardiovascular medicine 7:567837 PubMed33330641

2020

Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Ackers,Candice Szymanski,Mitchell J Silver,Ramiro Malgor

Cell cycle (Georgetown, Tex.) 19:3231-3248 PubMed33198579

2020

Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Wen,Chenjiang He,Qin Zhang,Fanglin Zhang,Na Li,Yan Pan,Mengting Deng,Yue Wang,Jianping Li,Jiaxuan Qiu

Molecular oncology 14:462-483 PubMed31785055

2019

Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Tian,Yuanyuan Wu,Yating Yang,Jiaxin Wang,Menglan Niu,Shanjun Gao,Tao Qin,Dengke Bao

Cell communication and signaling : CCS 17:109 PubMed31462314

2019

WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojie Jiang,Miaofeng Zhang,Yanhua Zhang,Weiping Zhou,Tao Zhu,Qing Ruan,Hui Chen,Jie Fang,Fei Zhou,Jihong Sun,Xiaoming Yang

Oncology reports 41:3137-3147 PubMed30896857

2019

microRNA‑485 inhibits the malignant behaviors of retinoblastoma by directly targeting Wnt3a.

Applications

Unspecified application

Species

Unspecified reactive species

Xueman Lyu,Ling Wang,Jia Lu,Hong Zhang,Lina Wang

View all publications

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